Treatment with neutrotrophic factors may prevent neurogenic bladder dysfunction
| Project/Area Number |
12671529
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
WAKABAYASHI Yoshihiko Shiga University of Medical Science, Department of Urology, Assistant Professor, 医学部, 講師 (80191724)
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| Co-Investigator(Kenkyū-buntansha) |
KIM Chul Jang Shiga University of Medical Science, Department of Urology, Assistant Professor, 医学部, 講師 (10204968)
YOSHIKI Tatsuhiro Shiga University of Medical Science, Department of Urology, Assistant Professor, 医学部, 助教授 (80230704)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2000: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Neurogenic Bladder / Nerve Growth Factor / Neurotrophic Factor / Molecular Biology |
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| Research Abstract |
1. We examined the expression of NGF family mRNAs in the rat urinary bladder during pre- and postnatal development using competitive RT-PCR. The mRNA levels showed a biphasic pattern of expression ; one peak was at prenatal ages (E15-18) and the other peak was at postnatal ages (P14-P28). In addition, we also examined the development of two different classes of transmembrane receptor proteins, the Trk family of tyrosine kinases and neurotrophin receptor p75 immunoreactivities. Both Trk receptor-positive and p75 neurotrophin receptor-positive fibers were also first seen at E16. The number of Trk immunoreactive nerves reached a peak at E18. In contrast, p75-labeled fibers were abundant at E18-P14. These data suggest that neurotrophins are involved in the mechanisms of bladder nerve growth for the prenatal period and reorganization of bladder reflex pathways. (Wakabayashi Y et al. 2002 ; Kawakami T et al. 2002) Glial cell-line derived neurotrophic factor (GDNF), and related factors neurturin (NRTN) are members of the GDNF family of neurotrophic factors. GDNF mRNA levels at E15 were the highest of all the stages we examined, and then immediately decreased. In contrast, NRTN mRNA levels did not change between E15 and postnatal day 14, and thereafter they gradually, but insignificantly, increased. Our results suggest that GDNF and NRTN may act as trophic factors for neural in-growth to the bladder and/or for the maintenance of mature neurons innervating the bladder. (Kawakami T et al. in press)
2. We examined the expression of NGF family mRNAs in a rat model of nerve regeneration in the bladder. The mRNA levels at 21 days postoperatively were the highest of all the stages. Results suggest that injection of NGF family in the bladder during early postoperative period results in more rapid nerve regeneration.
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Report
(3 results)
Research Products
(7 results)
