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Research on gonococcal resistance to antibiotics including new quinolone

Research Project

Project/Area Number 12671542
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionKyushu University

Principal Investigator

TANAKA Masatoshi  Department of Urology Associate professor, 大学院・医学研究院, 助教授 (30171797)

Co-Investigator(Kenkyū-buntansha) 持田 蔵  九州大学, 医学部・附属病院, 助手 (80315069)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
KeywordsNeisseria gonorrhoeae / prevalence rate / quinolone resistance / tetracycline resistance / penicillin resistance / cephem resistance / penicillinase-produding / Mechanism of resistance / 薬剤感受性 / 薬剤耐性 / DNAジャイレース / トポイソメラーゼIV / ペニシリ耐性 / 栄養要求型
Research Abstract

I. Prevalence of antibiotic-resistant Neisseria gonorrhoeae : We determined the MIC for 208 N. gonorrhoeae isolates in 2001 in Fukuoka city. The prevalence rates of quinolone resistance (CPFX MIC≧1 μg/ml), penicillin resistance (PCG MIC≧2 μg/ml), and tetracycline resistance (TC MIC≧2 μg/ml) in those isolates were 64.4%, 13.4%, and 13.9%, respectively. The prevalence rate of penicillinase-producing N. gonorrhoeae (PPNG) was only 1.4% in those isolates. There were no isolates resistant to spectinomycin (SPCM MIC≧128 μg/ml) or intermediately resistant to cefixime (CFIX MIC≧0.5 μg/ml) in those isolates. However, the prevalence rate of reduced susceptibility to CFIX with MIC of 0.125 to 0.25 μg/ml was relatively higher (23.6%) in 2001 as compared with that (1.6%) in 1995-96. These results indicate that the prevalence rate of quinolone resistance in the isolates in 2001 was remarkably high and that the isolates in 2001 showed reduced susceptibility to CFIX.
II. Mechanism of resistance to cefi … More xime : We examined the genetic mechanisms of chromosomally mediated resistance to CFIX. Chromosomally mediated penicillin (a variety of β-lactam antibiotics) resistance in N. gonorrhoeae occurs in part through alterations in penicillin-binding proteins (PBPs) and a decrease in outer membrane permeability. The first step in transformation to high-level penicillin resistance is mediated by the penA gene, which encodes altered forms of PBP2. Therefore, we analyzed mutations in the penA gene of the 6 gonococcal isolates intermediately resistant (CFIX MIC=0.5 μg/ml) and 3 susceptible (CFIX MIC; ≦0.001 to 0.004 μg/ml) to CFIX, PCR and direct DNA sequencing were performed to identify mutations in a region including the transpeptidase domain of the penA gene. All 6 isolates intermediately resistant to CFIX had same 33 amino acid substitutions through position 323 to 480 within the region of the transpeptidase domain of PBP2. However, no single alteration was identified in the same region of the PBP2 of the 3 susceptible isolates. Our data suggest that multiple alterations in PBP2 play an essential role in conferring cefixime resistance to N. gonorrhoeae. Less

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Tanaka M, (他7名): "A remarkable reduction in the susceptibility of Neisseria gonorrhoeae isolates to cephems and the selection of antibiotic regimens for the single-dose treatment of gonococcal infection in Japan"J Infect Chemother. 8. 81-86 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 田中正利, (他10名): "淋菌感染症の治療に関する臨床的および基礎的検討"西日泌尿. 64. 324-337 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 田中正利: "STDと薬剤耐性-淋菌-"日性感染症会誌. 13. 44-58 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tanaka M, et al.: "A remarkable reduction in the susceptibility of Neisseria gonorrhoeae isolates to cephems and the selection of antibiotic regimens for the single-dose treatment of gonococcal infection in Japan"J Infect Chemother. 8. 81-86 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tanaka M, et al.: "Clinical and fundamental investigations on the treatment of Neisseria gonorrhoeae infections"Nishinihon J Urol. 64. 324-337 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tanaka M: "STD and antimicrobial resistance- Neisseria gonorrhoeae"J J STD. 13. 44-58 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tanaka M, (他7名): "A remarkable reduction in the susceptibility of Neisseria gonorrhoeae isolates to cephems and the selection of antibiotic regimens for the single-dose treatment of gonococcal infection in Japan"J Infect Chemother. 8. 81-86 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 田中正利, (他10名): "淋菌感染症の治療に関する臨床的および基礎的検討"西日泌尿. 64. 324-337 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 田中正利: "STDと薬剤耐性-淋菌-"日性感染症会誌. 13. 44-58 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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