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THE ADHERENCE OF CALCIUM OXALATE CRYSTAL ON UROTHELIUM AND CYTOKINE PRODUCTION

Research Project

Project/Area Number 12671554
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionKeio University

Principal Investigator

NAKAJIMA Fumio  Keio University School of Med. Urology, Lecturer, 医学部, 専任講師 (10159064)

Co-Investigator(Kenkyū-buntansha) SATOH Akinori  Keio University School of Med. Urology, Staff, 医学部, 助手 (00296675)
MURAI Masaru  Keio University School of Med. Urology, Professor, 医学部, 教授 (90101956)
NAKASHIMA Jun  Keio University School of Med. Urology, Lecturer, 医学部, 専任講師 (10167546)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2001: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordscalcium oxalate / urothelium / adherence / cytokine / allopurinol / 結晶付着 / 細胞障害
Research Abstract

It is known that oxalate and calcium oxalate monohydrate (COM) crystals induce urothelium injury. Although xanthine oxidase inhibitor (allopurinol, oxypurinol) can protect the cell injury which is induced by oxalate and calcium crystals, whether xanthine oxidative inhibitor can inhibit crystal adhesion onto urothelium remains unclear. Therefore we investigate whether xanthine oxidative inhibitor can inhibit adhesion of COM on urotheium cells. On the basic experiment, the adhesion of COM seed onto urothelium cell lines were found to be more in the growth state than the static state of the cell lines. The cell lines used were MDCK, LLCPK_1 and HK-2. These cells were cultured to confluent on round glass cover slips in a 12-well culture plate in MEM with 10% FCS at 37 ℃ and 5% C0_2. These cells were incubated at the presence or absence of 0.2 or 2 mmol. of allopurinol or oxypurinol for 4 hours before attachment with COM crystal. One mg/ml of ^<14>C COM crystal suspension were added and incubated for 10 minutes. Non-adherent crystals were washed in PBS and adherent radioactive crystals were measured by liquid scintillation counter. The cytotoxicity induced by COM crystal was measured by LDH release assays. Allopurinol and oxypurinol significantly reduce the COM crystal adhesion in dose dependent manner. Two mmol. of allopurinol and oxypurinol showed approximately 60% of adhesion inhibition. Allopurinol and oxypurinol also prevent the release of LDH from COM injured cell lines. However, only 10 minutes incubation with COM seed showed less than 2% of cytotoxicity. Therefore the inhibition of cell cytotoxicity caused by COM is not the major factor in anti-adherence effect of allopurinol. Xanthine oxidative inhibitor can prevent the COM crystal adhesion on urothelium. Therefore xanthine oxidative inhibitor may be expected as an agent for prevention of urolithiasis. Regarding the cytokine production by urothelium cell lines, we can not show any evidence.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] 長妻克己他: "尿路上皮細胞の蓚酸カルシウム結晶付着能の検討"日本尿路結石研究会 第10回学術集会記録集. 82-85 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 長妻克己他: "種々の尿路上皮細胞株におけるCaOx結晶付着能の検討"日本尿路結石研究会 第11回学術集会記録集. 211-218 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 長妻克己他: "ヒト尿細管由来培養細胞株を用いた蓚酸カルシウム結晶付着能の検討"日本泌尿器科学会雑誌. 92. 314-314 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katsumi Nagatsuma et al.: "Investigation of COM seed adherence onto urothelium"Japanese society of urolithlasis research. The 11th annual meeting abstracts. 82-85 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katsumi Nagatsuma et al.: "COM seed adherences on various urothelium cell lines"Japanese society of urolithlasis research. The 12th annual meeting abstracts. (in press). 211-218 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 長妻克己他: "種々の尿路上皮細胞株におけるCaOx結晶付着能の検討"日本尿路結石研究会 第10回学術集会記録集. 211-218 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 長妻 克己他: "ヒト尿細管由来培養細胞株を用いた蓚酸カルシウム結晶付着能の検討"日本泌尿器科学会雑誌. 92. 314-314 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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