| Project/Area Number |
12671570
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Fukuoka University |
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Principal Investigator |
ARIYOSHI Asami School of Medicine, Fukuoka University, Professor, 医学部, 教授 (00078768)
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| Co-Investigator(Kenkyū-buntansha) |
TOMITA Yoshihiro School of Medicine, Fukuoka University, Instructor, 医学部, 助手 (60341434)
KANEGAE Shigehiro School of Medicine, Fukuoka University, Instructor, 医学部, 助手 (10291840)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2000: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Ileal conduit / Cisplatin / Inferior vena cava / Portal vein / Rat model |
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| Research Abstract |
1. LONG-TERM EXPERIMENTAL MODEL ; At first, we intended to make ten rat models of ileal conduit urinary diversion for a long-term experimental observation. Although 51 rats were operated, most of them died of bleeding due to self-injury of the urinary stom a with their teeth. So we altered our initial plan to a new experiment as shown below.
2. ACUTE EXPERIMENTAL MODEL ; Materials and Methods : Six male Wistar rats weighing 350〜500g were used for the ileal conduit model. A 10-cm ileal segment was attached to the bladder with a handmade connector and the distal end was opened on the abdomen to create a urinary stoma. The ileal segment was not connected to the bladder in 6 control rats. A bolus of cisplatin (1 mg/kg B. W.) was intravenously administered. After 20 minutes, blood sampling was done from both inferior vena cava (IVC) and portal vein (PV). Urine was collected for 20 minutes after the injection. Results : Body weight, urine volume, urinary concentration, excreted volume and excretion rate of cisplatin in urine showed no difference between the ileal conduit and the control (p=0.44, p=0.70, p=0.53, p=0.92, p=0.98). No difference was also observed in the blood cisplatin consentration between IVC and PV. But the cisplatin concentration ratio between PV and IVC (P/I ratio) was larger in the ideal conduit group (p=0.04) than that of the control group.
Discussion : Two possibilities are considered on the difference between cisplatin concentration of IVC and PV. One may be much water reabsorption from the ileal conduit. The other may be a shorter time of higher cisplatin concentration in urine than that in blood. This might result in less passive transport (reabsorption) of cisplatin from the ileal conduit. Further long-term experimental observation will be needed for pharmacological significance of cisplatin reabsorption from the intestine incorporated in the urinary tract.
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