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Regulation of Prolactin Secretion from Pituitary Lactotrophs in the Presence of Activin

Research Project

Project/Area Number 12671577
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionGunma University

Principal Investigator

ABE Yumiko  Gunma Univ. Sch. Med. Dept. OB/GYN, 医学部, 助手 (70261857)

Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
KeywordsActivin / Follistatin / Inhibin / Prolactin / Growth Hormone / Pituitary
Research Abstract

It has been reported that activin suppressed the synthesis and secretion of prolactin (PRL) and growth hormone (GH) using the primary anterior pituitary cell culture or cell lines derived from somatotrophs or lactotrophs. Recently, it was also reported that activin suppressed the synthesis of PRL but stimulated the synthesis of GH in GH3 cells derived from rat somatolactotroph. The culture media used in all of these experiments contained sera. Therefore, it was speculated that the growth factors and cytokines in sera interacted with activin in the above culture systems. Antibiotics were also present. Aminoglidosids are the antibiotics often used in cell culture and it was reported that aminoglycosides suppressed the hydrolysis of phosphoinositide. Therefore, not only growth factors and cytokines in sera but also antibiotics might affect the signal transduction pathways of activin. In order to study the effect of activin on the synthesis of PRL and GH without other factors interfering, we established the culture system of GH3 cells using a defined medium without the addition of sera or antibiotics. We found that activin stimulated the synthesis of both PRL and GH in this system. Time dependent and dose dependent stimulation was observed. The activin binding protein follistatin suppressed the increased synthesis of PRL and GH in the presence of activin in a dose dependent manner. We also found that MAP kinase inhibitors inhibited the increased synthesis of PRL and GH in the presence of activin. Activin may regulate the synthesis of PRL and GH by interacting with MAP kinase pathways.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Wang H, et al.: "Effect of adrenal and ovarian androgens on type 4 follicles unresponsive to FSH in immature mice"Endocrinology. 142. 4930-4936 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kikuchi N, et al.: "Inhibitory action of leptin on early follicular growth differs in immature and adult female mice"Biol-Reprod. 65. 66-71 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 安部由美子 他: "インヒビンとアクチビン"産婦人科の世界. 53増刊号. 28-36 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Minegishi T, et al.: "The mechanisms of retinoic acid-induced regulation on the follicle-stimulating hormone receptor in rat granulosa cells"Biochim-Biophys-Acta. 1495. 203-211 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kobayashi J, et al.: "Morphological assessment of the effect of growth hormone on preantral follicles from 11-day-old mice in an in vitro culture system"Biochem-Biophys-Res-Commun. 268. 36-41 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 安部由美子: "下垂体と成長因子"産婦人科の世界. 52. 3-7 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Wang H, Andoh K, Hagiwara H, Xiaowei L, Kikuchi N, Abe Y, Yamada K, Fatima R, Mizunuma H: "Effect of adrenal and ovarian androgens on type 4 follicles unresponsive to FSH in immature mice"Endocrinology. 142. 4930-4936 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kikuchi, -N, Andoh, -K, Abe, -Y, Yamada, -K, Mizunuma, -H, Ibuki, -Y: "Inhibitory action of leptin on early follicular growth differs in immature and adult female mice"Biol-Reprod. 65. 66-71 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Minegishi, -T, Hirakawa, -T, Kishi, -H, Abe ,-K, Tano, -M, Abe, -Y, Miyamoto, -K: "The mechanisms of retinoic acid-induced regulation on the follicle-stimulating hormone receptor in rat granulosa cells"Biochim-Biophys-Acta. 1495. 203-211 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Wang H. et al.: "Effect of adrenal and ovarian androgens on type 4 follicles unresponsive to FSH in immature mice"Endocrinology. 142. 4930-4936 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kikuchi N. et al.: "Inhibitory action of leptin on early follicular growth differs in immature and adult female mice"Biol-Reprod. 65. 66-71 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 安部由美子 他: "インヒビンとアクチビン"産婦人科の世界. 53増刊号. 28-36 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Minegishi T. et al.: "The mechanisms of retinoic acid-induced regulation on the follicle-stimulating hormone receptor in rat granulosa cells"Biochim-Biophys-Acta. 1495. 203-211 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kobayashi J. et al.: "Morphological assessment of the effect of growth hormone on preantral follicles from 11-day-old mice in an in vitro culture system"Biochem-Biophys-Res-Commun. 268. 36-41 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] 安部由美子: "下垂体と成長因子"産婦人科の世界. 52. 3-7 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] ibuki-Y et al: "In Viro Action of Fouistatin on Pituitary Testicular System."Program and Abstracts of The Endocrine society's Annual Meeting. 82. 245 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 伊吹友二 他: "アクチビンはプロラクチン産生を促進する"日本産科婦人科学会雑誌. 53. 255 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 安部由美子 他: "インヒビンとアクチビン"産婦人科の世界. 増刊号(in press). (2001)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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