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Analysis of a novel human protein WDC146 highly expressed in testis : for elucidation of the function during spermatogenesis

Research Project

Project/Area Number 12671602
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionOkayama University

Principal Investigator

SAKAI Akiko  Okayama University, Graduate School of Medicine and Dentistry, Assistant, 大学院・医歯学総合研究科, 助手 (60205698)

Co-Investigator(Kenkyū-buntansha) NOMURA Takako  Okayama University, Graduate School of Medicine and Dentistry, Assistant, 大学院・医歯学総合研究科, 助手 (20116437)
SASAKI Junzo  Okayama University, Graduate School of Medicine and Dentistry, Professor, 大学院・医歯学総合研究科, 教授 (30093686)
OUCHIDA Mamoru  Okayama University, Graduate School of Medicine and Dentistry, Associate professor, 大学院・医歯学総合研究科, 助教授 (80213635)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsWD40 repeat / collagen-like domain / human novel protein / testis-specific expression / cancer-testis gene / nuclear localization / homologs among wide species / spermatogenesis / WD40リピートタンパク質 / コラーゲン様ドメイン / 核局在タンパク質 / 精巣特異的 / がん・精巣遺伝子
Research Abstract

1) WDC146 is a novel human protein of 146 kDa which is expressed highly in testis. It is composed of three domains, a WD40 domain, a collagen-like domain and a GPR-rich repeat domain, from N-terminus to C-terminus, respectively.
2) WDC146 is highly expressed in testis both in human and mouse. The amino acid sequence showed significant homology between them overall (96%), including complete match (100%) for the region of WD40 domain. Even in Drosophila and yeast, we identified the sequence conserved in part.
3) WDC146 showed marked expression in pachytene spermatocytes, and nuclear and perinuclear localization was observed intracellularly.
4) The detailed analysis of the expression pattern of WDC146 during spermatogenesis is in progress, with the technical difficulty of fixation of testis applicable for in situ hybrization.
5) WD40 domain is known to work as the platform motif as the time of protein-protein interaction. The two-hybrid screening was executed repeatedly to detect interactive protein with WDC146, but not yet succeed.
6) Cancer-testis genes are defined as testis-specific genes also expressed in many tumors and tumor cell lines. WDC146 may be classified in them, as tumor cell lines tested all showed moderate expression of WDC146, though some tumors did not.
7) The WDC146 gene is mapped at 2q14-21 and consists of 22 exons. Besides the intact mRNA of about 4kb, a chimeric one result from mis-splicing always detected at about 1kb size.
8) The promoter region was identified within 0.5kb upstream region of exon 1.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Ito, S.: "A novel WD4O repeat protein, WDC146, highly expressed during spermatogenesis in a stage-specific manner"Biochem.Biophys.Res.Commun.. 280. 656-663 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ito S: "A novel WD40 repeat protein, WDC146, highly expressed during spermatogenesis in a stage-specific manner"BBRC. 280. 656-663 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ito, S.: "A novel WD4O repeat protein, WDC146, highly expressed during spermatogenesis in a stage-specific manner"Biochem.Biophys.Res.Commun.. 280. 656-663 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] ItO,S.: "A Novel WD40 Repeat Protein, WDC146, Highly Expressed during Spermatogenesis in a Stage-Specific Manner"Biochem.Biophys.Res.Commun.. 280(3). 656-663 (2001)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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