| Project/Area Number |
12671620
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Yokohama City University |
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Principal Investigator |
SAKAKIBARA Hideya Yokohama City University, Dept. OB/GY, Lecturer, 医学部附属病院, 講師 (60235140)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | interferon / endometrium / PKR / MxA / IRF-1 / IRF-2 / MXA / MxA |
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| Research Abstract |
Interferon is a cytokine and has various biological effects to many different kinds of cells. We already established that it inhibited the cell growth of endometrial cells using primary culture of human endometrial stromal cells. In order to examine the mechanism of action of interferon in human endometium, we investigated the expression of double-stranded RNA-dependent protein kinase (PKR), MxA, interferon regulatory factor-1 (IRF-1) and IRF-2, which were known to be the interferon induced proteins, in human endometrium. The results showed that all these mRNAs were expressed in human endometrium. We also examined the difference of the expression of these mRNAs among proliferative and secretory phase endometrium and deciduas in the early phase of pregnancy. The results showed that the changes of the mRNA expression during these periods were different among these proteins. It suggests that the expression of these proteins is controlled not only by interferon but also other effectors. Furthermore, we found that the expressions of these proteins were stimulated by interferon at dose and time dependent manner using primary culture of human endometrial stromal cells. From this result, these proteins seem to be involved in the action of interferon to endometrial stromal cells. These results suggest that these interferon induced proteins have some biological roles which is not controlled by interferon and are stimulated its expression by interferon when the action of interferon is needed, for example at the time of viral infection.
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