The study on role of TGF β in estrogen-induced endometrial carcinoma

Research Project

Project/Area Number	12671621
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Obstetrics and gynecology
Research Institution	Yokohama City University
Principal Investigator	TAKAHASHI Tsuneo Yokohama City University, Maternity & Neonate Center, Associate Professor, 医学部附属病院, 助教授 (60179497)
Co-Investigator(Kenkyū-buntansha)	HARUKI Atsushi Yokohama City University, Maternity & Neonate Center, Lecturer, 医学部附属病院, 助手 (90315797) ISHIKAWA Hiroshi Yokohama City University, Maternity & Neonate Center, Lecturer, 医学部附属病院, 助手 (70315795) IKEDA Mario Yokohama City University, Gynecology, Assistant Professor, 医学部附属病院, 講師 (50254173)
Project Period (FY)	2000 – 2002
Project Status	Completed (Fiscal Year 2002)
Budget Amount *help	¥3,300,000 (Direct Cost: ¥3,300,000) Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000) Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000) Fiscal Year 2000: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywords	TGF β / endometrial carcinoma
Research Abstract	We showed the gene expression of TGF β 1, TGF β 2, and TGF β 3 in the uterus stimulated by estrogen. To investigate the physiological role of these TGF β isoforms we did in situ hybridization and immunohistochemistry study. By these study the gene expression of TGF βs were recognized more potent in epithelium than stroma In addition, these gene expression was confirmed as protein expression by immunohistochemistry. If the protein appeared by estrogen had physiological action, a receptor must be present. And we showed the presence of the receptor that was necessary for biological action. In addition, these protein decreased when estrogen was administered, and it was suggested that these action were controlled at a receptor level The moleculer mechanism of expression of TGF β by estrogen is still unclear. However, gene expression by estrogen and protein expression and the presence of a receptor strongly suggest that these protein take an important role in action mechanism of estrogen.