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CD4 independent vertical HIV transmission and possible involvement of decidual immune cells

Research Project

Project/Area Number 12671634
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionNihon University

Principal Investigator

HAYAKAWA Satoshi  Nihon Univ., Dept.Infect Dis.Cont, Associate Professor, 医学部, 助教授 (30238084)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Tatsuo  Nihon Univ., Dept Obstet.Gynecol., Professor, 医学部, 教授 (40167721)
CHISHIMA Fumihisa  Nihon Univ., Dept Obstet.Gynecol., Assistant Professor, 医学部, 講師 (50277414)
ALEEMUZZAMAN Sheikh  Nihon Univ., Dept Pathology, Research fellow, 医学部, 助手 (10277436)
NEMOTO Norimichi  Nihon Univ., Dept Pathology, Professor, 医学部, 教授 (80096875)
HONDA Mitsuo  National Inst.Infect., AIDS research Center, Diseases Research group director, エイズ研究センター, 第一研究グループ長 (20117378)
西成田 進  日本大学, 医学部, 講師 (70110963)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2000: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsHIV / decidua / placenta / chemokines / NK cell / NKT cell / G-CSF / γδ T cell / 垂直感染 / 脱落膜免疫細胞 / _γδT細胞 / CD56^<bright>LGL / IL-16 / SHIV / エイズ / 胎盤関門 / アポトーシス / カニクイザル / 母児感染 / CD4 / ケモカイン・レセプター
Research Abstract

1 We investigated placenta samples obtained from HIV positive mothers. We observed positive staining of HIVp24 in villous trophoblasts, extravillous trophoblasts and Hofbauer cells by immunohistochemical techniques, though their babies were not infected with HIV.
2 Presence of HIV provirus DNA was confirmed with PCR in antigenically separated placental cells.
3 Human choriocarcinoma cell line JEG-3 was susceptible to cell free HIV and HIV infected T cell lines. Both R5-HIV and X4-HIV replicate in JEG-3.
4 HIV infected JEG-3 by CD4 independent but CCR5 or CXCR-4 dependent manner.
5 Replication of HIV in choriocarcinoma cell lines were inhibited by various chemokines including MIP-1alpha and RANTES.
6 Freshly isolated placental trophoblasts and choriocarcinoma cell lines express functional chemokine receptors.
7 Chemokines regulate cell proliferation and hCG production of choriocarcinoma cell lines.
8 HIV infected choriocarcinoma cell line JEG-3 became susceptible to IL-2 activateddecidual CD56 positive large granular lymphocytes(LGL).
9 G-CSF and TGF-β produced by trophoblasts suppressed IL-2/IL-12 activation of CD56 positive LGL
10 CD56 positive LGL are susceptible to HIV but produce various chemokines and IL-16, Which possibly protect these cells and trophoblasts from HIVinfection
11 γδ T cells in deciduas produce large amount of TGF-β and IL-10 which possibly inhibit eradication of HIV infected cells by cytotoxic T cells.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (41 results)

All 2003 2002 2001 2000 Other

All Journal Article (22 results) Book (3 results) Publications (16 results)

  • [Journal Article] Granulocyte colony stimulation factor (G-CSF) suppresses Interleukin(IL)-12 and/or IL-2 induced Interferon (IFN)-γ production and cytotoxicity of decidual mononuclear cells.2003

    • Author(s)
      Sugita K, Hayakawa S, Karasaki-Suzuki M, Hagiwara H, Chishima.F, Nishinarita S, Yamamoto T.:
    • Journal Title

      American Journal of Reproductive Immunolog 50

      Pages: 83-89

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] 子宮局所免疫細胞の特長2003

    • Author(s)
      川口里恵, 小沢真帆, 早川 智, 田中忠夫
    • Journal Title

      臨床婦人科産科 57

      Pages: 1022-1030

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] 粘膜における免疫応答とアレルギーの分子機構2003

    • Author(s)
      羅 智靖, 早川 智
    • Journal Title

      産婦人科の実際 52

      Pages: 673-682

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Granulocyte colony stimulation factor (G-CSF) suppresses Interleukin(IL)-12 and/or IL-2 induced Interferon (IFN)-γ production and cytotoxicity of decidual mononuclear cells.2003

    • Author(s)
      Sugita K, Hayakawa S, Karasaki-Suzuki M, Hagiwara H, Chishima.F, Nishinarita S, Yamamoto T.
    • Journal Title

      American Journal of Reproductive Immunology 50

      Pages: 83-89

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Mechanisms of immune privilege for tumor cells by regulatory cytokines produced by innate and acquired immune cells.2002

    • Author(s)
      Seo N, Hayakawa S, Tokura N
    • Journal Title

      Seminar in Cancer Biology 12

      Pages: 291-300

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] A Th2 chemokine, TARC, produced by trophoblasts and endometrial gland cells, regulates the infiltration of CCR4+ T lymphocytes into human decidua at early pregnancy.2002

    • Author(s)
      Tsuda H, Michimata T, Hayakawa S, Tanebe K, Sakai M, Fujimura M, Matsushima K, Saito S.
    • Journal Title

      American Journal of Reproductive Immunology 48

      Pages: 1-8

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] 妊娠とウイルス 感染.2002

    • Author(s)
      早川 智, 椙田賢司, 小峯志保子, 千島史尚, 本多三男, 山本樹生
    • Journal Title

      産婦人科治療 84

      Pages: 1135-1140

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] 妊娠の維持と脱落膜TGF-β2002

    • Author(s)
      早川 智, 瀬尾尚宏, 太田啓明, 千島史尚, 山本樹生.
    • Journal Title

      臨床免疫 38

      Pages: 26-30

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] The herbal medicine shosaiko-to exerts different modulating effects on lung local immune responses among mouse strains.2002

    • Author(s)
      Ohtake N, Nakai Y, Yamamoto M, Ishige A, Sasaki H, Fukuda K, Hayashi S, Hayakawa S.
    • Journal Title

      International Immunopharmacology 2

      Pages: 357-366

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Increased expression of cyclooxygenase-2 in the local lesions of endometriosis patients2002

    • Author(s)
      Chishima F, Hayakawa S, Kinukawa N, Aleemuzamann S, Nemoto N, Yamamoto T, Honda M
    • Journal Title

      American Journal of Reproductive Immunology 48

      Pages: 50-56

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] A Th2 chemokine, TARC, produced by trophoblasts and endometrial gland cells, regulates the infiltration of CCR4+T lymphocytes into human decidua at early pregnancy.2002

    • Author(s)
      Tsuda H, Michimata T, Hayakawa S, Tanebe K, Sakai M, Fujimura M, Matsushima K, Saito S.
    • Journal Title

      American Journal of Reproductive Immunology 48

      Pages: 1-8

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Interleukin-10 expressed at early tumour sites induces subsequent generation of CD4(+) T-regulatory cells and systemic collapse of antitumour immunity.2001

    • Author(s)
      Seo N, Hayakawa S, Takigawa M, Tokura Y
    • Journal Title

      Immunology 103

      Pages: 449-57

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Significance of decidual NK and NKT cells in normal pregnancy and abortion.2001

    • Author(s)
      Yamamoto T, Hayakawa S, Chishima, Nagaishi M
    • Journal Title

      Nihon University Journal of Medicine 43

      Pages: 167-177

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] 生殖免疫学から見たHIV母児感染2001

    • Author(s)
      早川 智, 永石(石井)真木, 千島史尚, 鈴木(唐崎)美喜, 山本樹生, 本多三男, 吉野直人, 仲宗根正, 原敬司, 泉康之, 網康至, 小峯志保子, シェイク・アリムザマン, 根本則道
    • Journal Title

      日本生殖免疫学会雑誌 16

      Pages: 1-6

    • NAID

      130006907689

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Interleukin-1O expressed at early tumor sites induces subsequent generation of CD4(+) T-regulatory cells and systemic collapse of antitumour immunity.2001

    • Author(s)
      Seo N, Hayakawa S, Takigawa M, Tokura Y
    • Journal Title

      Immunology 103

      Pages: 449-457

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Expression of functional chemokine receptors in human placental cells and choriocarcinoma cell lines2000

    • Author(s)
      Ishii M, Hayakawa S, Yoshino N, Honda M, Nishinarita S, Satoh K.
    • Journal Title

      American Journal of Reproductive Immunology 44

      Pages: 365-73

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Murine fetal resorption and experimental pre-eclampsia are induced by both excessive Th1 and Th2 activation2000

    • Author(s)
      Hayakawa S, Fujikawa T, Fukuoka H, Chisima F, Karasaki-Suzuki M, Ohkoshi E, Ohi H, Kiyoshi Fujii T, Tochigi M, Satoh K, Shimizu T, Nishinarita S, Nemoto N, Sakurai I.
    • Journal Title

      Journal of Reproductive Immunology 47

      Pages: 121-38

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Increased cytotoxicity of peripheral and decidual lymphocytes against choriocarcinoma cell lines in the patients with preeclampsia.2000

    • Author(s)
      Nagai N, Hayakawa S, Yamamoto T
    • Journal Title

      Nihon University Journal of Medicine 42

      Pages: 319-326

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Frequent presence of Helicobacter pylorigenome in the saliva of patients with hyperemesis gravidarum.2000

    • Author(s)
      Hayakawa S, Nakajima N, Karasaki Suzuki M, Yoshinaga H, Arakawa Y, Satoh K, Yamamoto T.
    • Journal Title

      American Journal of Perinatology 17

      Pages: 243-247

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Tumour infiltrating lymphocytes of uterine cervical carcinoma use skewed TCR Vβ gene segments but possess low prognostic values.2000

    • Author(s)
      Kanaeda T, Hayakawa S, Yamamoto T
    • Journal Title

      Nihon University Journal of Medicine 42

      Pages: 327-337

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Increased apoptosis and repair hyperplasia of the villous trophoblasts in preeclamptic placentae.2000

    • Author(s)
      Koide K, Hayakawa S, Satoh K, Yamamoto T
    • Journal Title

      Nihon University Journal of Medicine 42

      Pages: 251-264

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Characterization of NKT cells in human peripheral blood and decidual lymphocytes.

    • Author(s)
      Tsuda H, Sakai M, Michimata T, Tanebe K, Hayakawa S, Saito S
    • Journal Title

      American Journal of Reproductive Immunology 45

      Pages: 295-302

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Book] 妊娠の生物学(妊娠におけるTh1/Th2バランス)2001

    • Author(s)
      早川 智(中山徹也, 牧野恒久, 高橋迪雄 監修)
    • Total Pages
      396
    • Publisher
      永井書店(大阪)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Book] 新女性医学体系 24 妊娠中毒症(免疫学的異常)2001

    • Author(s)
      早川 智, 山本樹生(武谷雄二 総編集)
    • Total Pages
      342
    • Publisher
      中山書店(東京)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Book] 新女性医学体系2 妊娠・分娩・産褥の生理と異常(免疫系妊娠維持と生体防御)(pp97-109)2001

    • Author(s)
      早川 智, 山本樹生(武谷雄二総編集)
    • Total Pages
      382
    • Publisher
      中山書店(東京)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ishii M, Hayakawa S, Yoshino N, Honda M, Nishinarita S, Satoh K: "Expression of functional chemokine receptors in human placental cells and choriocarcinoma cell lines"American Journal of Reproductive Immunology. 44. 365-373 (2000)

    • Related Report
      2002 Annual Research Report
  • [Publications] Seo N, Hayakawa S, Takigawa M, Tokura Y: "Interleukin-10 expressed at early tumour sites induces subsequent generation of CD4(+) T-regulatory cells and systemic collapse of autitumour immunity"Immunology. 103. 449-57 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tsuda H, Sakai M, Michimata T, Tanabe K, Hayakawa S, Saito S: "Characterization of NKT cells in human peripheral blood and decidual lymphocytes"American Journal of Reproductive Immunology. 45. 295-302 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Seo N, Hyakawa S, Tokura N: "Mechanism of immune provilege for tumor cells by regulatory cytokines produced by innate and acquired immune cells"Seminars in Cancer Biology. 12. 291-300 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tsuda H, Michimata T, Hayakawa S, Tanabe K, Sakai M, Fujimura M, Matsushima K, Saito S.: "A Th2 chemokine, TARC, produced by trophoblasts and endometrial gland cells, regulates the infiltration of CCR4+ T lymphocytes into human decidua at early pregnancy"American Journal of Reproductive Immunology. 48. 1-8 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 早川 智, 山本樹生: "新女性医学体系 2 妊娠・分娩・産褥の生理と異常 P97-109 免疫系 妊娠維持と生体防御"中山書店(東京). 382 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 早川 智, 永石(石井)真木, 千島史尚, 鈴木(唐崎)美喜, 山本樹生, 本多三男, 吉野直人, 仲宗根正, 原敬司, 泉康之, 網康至, 小峯志保子, シェイク・アリムザマン, 根本則道: "生殖免疫学から見たHIV母児感染"日本生殖免疫学会雑誌. 16(1). 1-6 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ishii M, Hayakawa S, Suzuki MK, Yoshino N, Honda M, Nishinarita S, Chishima F, Nagaishi M, Satoh K.: "Expression of functional chemokine receptors of human placental cells"Am J Reprod Immunol. 44(6). 365-373 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Seo N, Hayakawa S, Takigawa M, Tokura Y: "Interleukin-10 expressed at early tumour sites induces subsequent generation of CD4(+) T-regulatory cells and systemic collapse of antitumour immunity"Immunology. 103(4). 449-457 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tsuda H, Sakai M, Michimata T, Tanebe K, Hayakawa S, Saito S.: "Characterization of NKT cells in human peripheral blood and decidual lymphocytes"Am J Reprod Immunol. 45(5). 295-302 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Chishima F, Hayakawa S, Sugita K, Kinukawa N, Aleemuzzaman S, Nemoto N, Yamamoto T, Honda M: "Increased expression of cyclooxygenase-2 in local lesions of endometriosis patients"Am J Reprod Immunol. 46(In press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 早川智, 山本樹生(分担執筆), 武谷雄二 総編集: "新女性医学体系 2 妊娠・分娩・産褥の生理と異常 免疫系 妊娠維持と生体防御 (p97-109)"中山書店(東京). 382 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 早川智(分担執筆), 中山徹也, 牧野恒久, 高橋迪雄 監修: "妊娠の生物学 妊娠におけるTh1/Th2バランス (p159-164)"永井書店(大阪). 396 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ishii M,Hayakawa S,Yoshino N,Honda M,Nishinarita S and Satoh K: "Expression of functional chemokine receptors in human placental cells and choriocarcinoma cell lines"Am.J.Reprod.Immunol.. 44. 365-373 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hayakawa S.Yoshino N,Honda M,Nemoto N,Yamamoto T: "Placental pathology of HIV infected mothers in Japan."Virchow's Archive. (印刷中).

    • Related Report
      2000 Annual Research Report
  • [Publications] Yoshino N,Kita T,Takano M Tsukahara Y,Hayakawa S. et al.: "Epidemiological study of human immunodeficiency virus-1 screening test for pregnant women in Japan"AIDS. (印刷中).

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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