| Project/Area Number |
12671636
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | KANAZAWA MEDICAL UNIVERSITY |
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Principal Investigator |
YAMAGUCHI Nobuo Kanazawa Medical University, school of medicine, professor, 医学部, 教授 (10106916)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMIZU Shoji Kanazawa Medical University, school of medicine, assistant professor, 医学部, 助教授 (30150759)
SUZUKI Nobutaka Kanazawa Medical University, school of medicine, research assitant, 医学部, 助手 (60251930)
IKAWA Hiromichi Kanazawa Medical University school of medicine, professor, 医学部, 教授 (20124935)
KAMEI Tsutomu Shimane Institute of Health Science, research major, 主任研究員 (90233965)
ZONG. Zhi-ping Kanazawa Medical University, school of medicine, lecturer, 医学部, 助手 (80216572)
山口 正晃 金沢大学, 理学部, 助教授 (60182458)
松井 健一郎 金沢医科大学, 医学部, 助手 (70288273)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2001: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2000: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Gestation / materno-foetal relation / immune suppression / MHC-restriction / cell traffic / H-2 gene / DNA finger printing / PCR |
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| Research Abstract |
We have previously reported that the immunization of pregnant mice with T-dependent antigens successfully induced suppression of the antigen-specific plaque forming cell (PFC) response to the relevant antigens in the offspring. This suppression was not due to the administered antigens, the antibodies produced by the pregnant mother, or lactational transfer, but was dependent on the presence of the intact maternal T cells. It was MHC-restricted manner tolerance and was able to continue at least 1/6 of the murine life. In traditional point of view, the placenta would act as a natural barrier and not allow the cells to pass through. However, the results we got strongly suggested that maternal T cells go through the placenta and then induce the tolerance. In this research project, we try to substantiate the presence of maternal cells in the fetal circulation through the use of molecular techniques. We have found that a highly polymorphic microsatellite sequence within the class II Eb gene of the H-2 complex is quite useful for the molecular detection of various H-2 alleles. For tracking maternal H-2, alleles in baby spleens, the method of DNA polymorphic analysis was carried out. The main procedure involved the PCR-amplification and RFLP analysis of the DNA sequence encompassing the H-2 specific microsatellite from the genomic DNA of baby mice. The results indicated that maternal T cells of immunized pregnant mice go through the placenta and into the fetal bodies, induce the antigen specific immunological tolerance in the offspring.
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