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Direct analysis of the variability of the particles located in the lateral membrane of the outer hair cells and transduction mechanism using atomic force microscopy

Research Project

Project/Area Number 12671646
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionTohoku University

Principal Investigator

KOIKE Takuji (2001)  Graduate School of Engineering, Tohoku Univ., Lecturer, 大学院・工学研究科, 講師 (10282097)

欠畑 誠治 (2000)  東北大学, 医学部・附属病院, 助手 (90261619)

Co-Investigator(Kenkyū-buntansha) 小池 卓二  東北大学, 大学院・工学研究科, 講師 (10282097)
大島 猛史  東北大学, 医学部・附属病院, 助手 (40241608)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2000: ¥2,500,000 (Direct Cost: ¥2,500,000)
Keywordscochlear outer hair cell / cytoskeleton / electromotility / atomic force microscopy / 運動能 / stiffness
Research Abstract

The ultrastructure of the OHC lateral wall was investigated by atomic force microscopy. When the position along the cell axis is represented by the normalized distance from the basal end and the positions of the basal and apical ends are expressed by 0.0 and 1.0, the following conclusions can be drawn.
1. The tapping mode imaging was used in the experiments, because frictional forces are minimized. The particles located in the plasma membrane were not able to be detected. Instead, circumferential filaments were observed, which appear to be actins that are part of the cortical lattice.
2. The mean interval of the circumferential filaments in the region between 0.0 and 0.85 from the basal end and that in the region between 0.85 and 1.0 from the basal end are 54 nm and 44 nm, respectively.
3. The difference between the intervals of the circumferential filaments in the region between 0.0 and 0.85 and between 0.85 and 1.0 from the basal end is one factor that causes the high stiffness in the apical region of the cell.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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