| Project/Area Number |
12671662
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
TSUJI Jun (2002) Department of Otolaryngology - Head and Neck Surgery, Kyoto University Graduate School of Medicine, Assistant, 医学研究科, 助手 (30252448)
村井 紀彦 (2001) 京都大学, 医学研究科, 助手 (30324627)
長谷部 誠司 (2000) 京都大学, 医学研究科, 助手 (40293873)
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| Co-Investigator(Kenkyū-buntansha) |
HIRANO Tomoo Department of Biophysics, Graduate School of Science, Kyoto University, Professor, 理学研究科, 教授 (50181178)
NAITO Yasushi Department of Otolaryngology - Head and Neck Surgery, Kyoto University Graduate School of Medicine, Lecturer, 医学研究科, 講師 (70217628)
村井 紀彦 京都大学, 医学研究科, 助手 (30324627)
辻 純 京都大学, 医学研究科, 助手 (30252448)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2002: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | delta-2 subunit of glutamate receptor / vestibular compensation / unilateral labyrinthectomy / vestibulo ocular reflex / c-Fos / medial vestibular nucleus / inferior vestibular nicleus / prepositus hypoglossal nucleus / 前庭眼反射 / グルタミン酸受容体δ2サブユニット / 赤外線 / 前庭小脳 / プルキンエ細胞 / 視運動性眼振 / 遺伝子欠損マウス / 適応 |
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| Research Abstract |
Ionotrophic delta-2 subunit of glutamate receptor is specifically expressed only in cerebellar Purkinje cells in the nervous system, and mutant mice deficient in delta 2 proteins have been raised (Kashiwabuchi 1995).
These mice did nor exhibit long term depression (LTD), which is one form of synaptic plasticity (Hirano 1995). We examined the postural righting reflex and vestibulo ocular reflex (VOR) after unilateral labyrinthectomy (UL) in these mutant mice, to investigate the involvement of LTD in vestibular compensation. And we also examined the c-Fos expression after unilateral ladyrinthectomy in these mutant mice, to investigate the morphological change during the vestibular compensation.
After UL in the knock out mice deficient in delta 2 glutamate receptor subunit, the recovery of the postural righting reflex and VOR are retarded than normal mice, which suggests delta 2 glutamate receptor subunit plays an important role in the process of the vestibnlar compensation. After UL in the knock out mice deficient in delta 2 Glutamate Receptor Subunit, c-fos positive cells are greater in number in the contralateral sides in Mve and SpVe than normal, very few in the contralateral PrH and positive in ipsilesionai IO, contrary to normal mice. We assume that the in the knock-out mice which have dysfunctions in the floccules, the neurons in the vestibular nucleus on the contra latreal side became hyperactive because of the disinhibition from the cerebellum. And in knock out mice lacking control from the cerebellar floccules, c-fos was not activated in PrH on this side.
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