| Project/Area Number |
12671664
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Kagoshima University |
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Principal Investigator |
TAKEUCHI Toru Kagoshima University, Faculty of Medicine, Professor, 医学部, 教授 (00188161)
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| Co-Investigator(Kenkyū-buntansha) |
XU Baohui Kagoshima University, Faculty of Medicine, Research Associate, 医学部, 助手 (00264408)
AOYAMA Koji Kagoshima University, Faculty of Medicine, Assistant Professor, 医学部, 講師 (70117472)
MIYAGUCHI Mamoru Higashiosaka city general hospital, Otorhinolaryngology, Head, 耳鼻咽頭科, 部長 (70166130)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2001: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Reactive Oxygen Species / Oxidative DNA damage / Cancer in the rhinosinus / Chronic inflammation / Carcinogenesis / Human |
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| Research Abstract |
We have established a method to determine a typical oxidative DNA damage, 8-hydroxydeoxyguanosine (8OHdG) in the rhinosinus mucosae. That is, extraction of DNA from the mucosae, digestion of DNA to nucleosides with nuclease P1 and alkaline phosphatase, ultra filtration of the nucleosides, HPLC separation of the nucleoside with reversed-phase column, and the detection of 8OHdG with electrochemical detector (ECD). We did the steps from DNA extraction to nucleosides ultra filtration in an anaerobic incubator to reduce the artificial formation of 8OHdG during sample processing.
With the method, we determined the levels of 8OHdG in the rhinosinus mucosae from the patients with chronic rhinosinusitis, nasal polyps and papilloma of rhinosinus, and compared the 8OHdG levels with those in normal rhinosinus mucosae. The diagnosis was done microscopically. The samples were obtained from the patients who had been scheduled to remove lesions by operation in Higashiosaka city hospital and had given us the informed consent.
The 8OHdG levels were 0.54 in normal mucosae(n=4), 0.59 in nasal polyp(n=7), 0.48 in chronic rhinosinusitis (n=7), and 0.43 in papilloma (n=3). We could not find significant difference between normal and other groups.
This is the first study to determine 8OHdG in rhinosinus mucosae. Ultrafiltration was effective to remove contaminants which interfere the 8OHdG analyses by HPLC-ECD, However we could not find significant increase of 8OHdG in chronic rhinosinusitis related samples. Recently the incidence of cancer in the rhinosinus is decreasing. From the present study we considered that the grades of chronic inflammation in rhinosinusitis are not severe enough to induce 8OHdG due to the improvement of the treatments and/or the host status.
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