| Project/Area Number |
12671668
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
HIRATA Shitau Hiroshima University, Medical Hospital, Assistant professor, 医学部・附属病院, 講師 (60263696)
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| Co-Investigator(Kenkyū-buntansha) |
ONO Kunihiko Hiroshima University, Medical Hospital, Clinical Assistant, 医学部・附属病院, 助手 (60335669)
福島 典之 広島大学, 医学部・附属病院, 講師 (70181261)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | head and neck squamous cell carcinoma / MCL1 / HKR1 / ZK7 / chemotherapy / ionizing radiation / ZK7遺伝子 / 頭頚部扁平上皮癌 / 治療抵抗性の獲得 / シスプラチン / 放射線 / mcl-1 / 治療抵抗性 |
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| Research Abstract |
Chemotherapy or radiotherapy has become a very important option for the treatment of head and neck squamous cell carcinomas (HNSCC) in order to preserve the patient's physical functions. For the aim to develop the more effective treatment than we have performed previously, we attempted to clarify the biological characteristics some genes in HNSCC. We evaluated expression of the BCL-2 family of genes by a RT-PCR assay in 16 samples of HNSCC and in corresponding normal head and neck mucosa (HNM). Expression levels of the MCL-1 gene were higher in HNSCC than in HNM. These results reveal that MCL1 plays an important role in molecular events for HNSCC progression. And we also evaluated expression of the ZK7 and HKR1genes in 38 HNSCC and 14 HNM samples. The expression levels of mRNA for ZK7 and HKR1 in HNSCC clinical specimens exposed to platinum drugs and/or ionizing radiation were found to be higher than those in non exposed specimens. Cell lines examination showed that ZK7 over-expressing cells had low sensitivity to the chemotherapeutic agent cisplatin and ionizing radiation. These results suggest that ZK7 may inhibit apoptotic cell death in HNSCC.
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