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Biological activity of head and neck cancer cell lines with regard to IL-6 and MMP

Research Project

Project/Area Number 12671679
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionJichi medical School

Principal Investigator

NISHINO Hiroshi  Jichi Medical School Assistant Profeddor, 医学部, 講師 (50245057)

Co-Investigator(Kenkyū-buntansha) ICHIMURA Keiichi  Jichi Medical School Professor, 医学部, 教授 (00010471)
ISHIKAWA Kazuhiro  Jichl Medical School Assistant, 医学部, 助手 (40296083)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsIL-6 / IL-6 receptor / RT-PCR / cell growth / cell invasion / G-CSF / 頭頸部癌 / γ線 / 自殺遺伝子 / アデノ随伴ウイルス / 単純ヘルペスチミジンキナーゼ / ガンシクロビル / MMP-2 / MMP-9
Research Abstract

We established NKO-1, a SCC cell line that produces high levels of G-CSF and IL-6, from a metastatic lymph node obtained from a patient with a poorly differentiated maxillary SCC. We studied the in vitro effect of G-CSF and IL-6 on the growth of NKO-1, the in vitro effect of neutralizing G-CSF on the growth of NKO-1 normally enhanced by rhG-CSF, and invasion assay. The in vitro growth of NKO-1 cells was enhanced by high concentrations of G-CSF, but not by any concentration of hIL-6. The effect of G-CSF on NKO-1 cell growth was reduced by antibody to G-CSF in a dose-dependent manner. Compared with other cell lines, which produce low levels of G-CSF and IL-6, NKO-1 cells showed strong invasiveness.
Cancer cells of head and neck cancer cell lines have IL-6 receptors (RT-PCR). The levels of those IL-6 receptors were reduced by antibody to IL-6 receptors in a dose-dependent manner. The in vitro growth rates of cancer cells of head and neck cancer cells lines were reduced by IL-6. We studied the invasiveness of cancer cells of head and neck cancer cell lines with IL-6 in an extracellular matrix membrane system. The degree of invasiveness was enhanced by incubating cells with IL-6. In addition G-CSF enhanced the invasiveness of tumor cells that produced large amounts of G-CSF.
Low efficiency of transgene expression can be a problem in some applications that require potent gene expression such as antitumor applications. We demonstrate that AAV vector harbouring the HSVtk is able to kill cancer cells more efficiently when used in combination with γ-ray irradiation. γ-rays enhance rAAV-mediated transegene expression.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (35 results)

All Other

All Publications (35 results)

  • [Publications] Hiroshi Nishino: "Combined therapy with conservative surgery, radiotherapy, and regional chemotherapy for maxillary sinus carcinoma"Cancer. 89(9). 1925-1932 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 西野宏: "切除不能な局所再発および転移頭頸部扁平上皮癌に対する化学療法"日耳鼻. 103(9). 963-969 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takeharu Kanazawa: "Pleomorphic adenoma of the pterygopalatine fossa"Eur Arch otorhinolaryngol. 257. 433-435 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 西野宏: "喉頭早期声門癌放射線治療後再発に対する機能保存手術"頭頸部腫瘍. 26(3). 422-425 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takeharu Kanazawa: "γ-Rays enhance rAAV-mediated transgene expression and cytocidal effect of AAV-HSVtk/ganciclovir on cancer cells"Cancer Gene Therapy. 8. 99-106 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takeharu Kanazawa: "Haemangiopericytoma of infratemporal fossa"The Journal of Laryngology and Otology. 115. 77-79 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 石川和宏: "自治医科大学における頭蓋底手術の適応と限界"頭頸部腫瘍. 27. 55-61 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 西野宏: "再発頭頸部癌患者に対する外来TS-1投与時の有害事象"癌と化学療法. 29(11). 2019-2023 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 西野宏: "stage III/IV舌癌再発症例の検討"頭頸部腫瘍. 28(3). 479-483 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hiroshi Nishino: "Results of orbital preservation for advanced malignant maxillary sinus tumors"Laryngoscope. (in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 西野 宏: "頭頸部MRI診断マニュアル、ENTONI No2"全日本病院出版会、編集企画夜陣紘治. 27-31 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 西野 宏: "耳鼻咽喉科診療プラクテイス 頭頸部腫瘍診断におけるdecision Making"分光堂、編集岸本誠司. 70-73 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 西野 宏: "耳鼻咽喉科診療Q&A"六法出版社 名古屋. 486-487 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hiroshi Nishino, Yoshirou Fujisawa, Takeharu Kanazawa, Kazuhiro Ishikawa, Mamoru Miyata, Keiichi Ichimura , Kouichi Abe, Hidetaka Tanaka: "Chemotherapy for unrebectable recurrent or metastatic squamous cell carcinoma of the head and neck"J Otolaryngol Jpn. 103(9). 963-969 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hiroshi Nishino, Mamoru Miyata, Kazuhiro Ishikawa, Takeharu Kanazawa, Keiichi Ichimura: "Combined therapy with conservative surgery, radiotherapy, and regional chemotherapy for maxillary sinus carcinoma"Cancer. 89(9). 1925-1932 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takeharu Kanazawa, Hiroshi Nishino, Keiichi Ichimura: "Pleomorphic adenoma of the pterygopalatine fossa : a case report"Eur Arch otorhinokaryngol. 257. 433-435 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hiroshi Nishino: "Fontolateral partial laryngectomy as salvage surgery for radiation failures in early glottic cancer"Head and Neck Cancer. 26(3). 422-425 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takeharu Kanazawa, Hiroshi Nishino, Mamoru Miyata, Ken Kuriki, Kouichi Abe, Keiichi Ichimura: "Haemangiopericytoma of infratemporal fossa"The Journal of Laryngology and Otology. 115. 77-79 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takeharu Kanazawa, Masashi Urabe, Hiroaki Mizukami, Takashi Okada, Akihiro Kume, Hiroshi Nishino, John Monahan, Ken Kitamura, Keiichi Ichimura, and keiya Ozawa: "γ-Rays enhance rAAV-mediated transgene expression and cytocidal effect of AAV-HSVtk/ganciclovir on cancer cells"Cancer Gene Therapy. 8. 99-106 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kazuhiro Ishikawa, Hiroshi Nishino, Keiichi Ichimura: "Indication for skull base surgery at Jichi Medical School"Head and Neck cancer. 27(1). 55-61 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hiroshi Nishino, Takeshi Shinozaki, Kazuhiro Ishikawa, Hidetaka Tanaka, Keiichi Ichimura: "Adverse reactions to TS-1 for outpatients with recurrent head and neck cancer"Jpn J cancer Chemotherapy. 29(11). 2019-2023 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hiroshi Nishino, Takeshi Shinozaki, Yoshirou Fuzisawa, Kazuhiro Ishikawa, Hidetaka Tanaka, Kouichi Abe, Keiichi Ichimura: "stage Results of treatment of stage III and IV tongue cancer"Head and neck cancer. 28(3). 479-483 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 西野宏, 篠崎剛, 石川和宏, 田中秀隆, 市村恵一: "再発頭頸部癌患者に対する外来TS-1投与時の有害事象"癌と化学療法. 29(11). 2019-2023 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 西野宏, 篠崎剛, 藤澤嘉郎, 石川和宏, 田中秀隆, 阿部弘一, 市村恵一: "stage III/IV舌癌再発症例の検討"頭頸部腫瘍. 28(3). 479-483 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takeharu Kanazawa, Masashi Urabe, Hiroaki Mizukami, Takashi Okada, Akihiro Kume, Hiroshi Nishino, John Monahan, ken Kitamura, keiichi Ichimura, keiya Ozawa: "γ-Rays enhance rAAV-mediated transgene expression and cytocidal effect of AAV-HSVtk/ganciclovir on cancer cells"Cancer Gene Therapy. 8. 99-106 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takeharu Kanazawa, Hiroshi Nishino, Mamoru Miyata, ken Kuriki, Kouichi Abe, Keiichi Ichimura: "Haemangiopericytoma of infratemporal fossa"The Journal of Laryngology and Otology. 115. 77-79 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 石川和宏, 西野宏, 市村恵一: "自治医科大学における頭蓋底手術の適応と限界"頭頸部腫瘍. 27. 55-61 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 西野 宏: "頭頸部MRI診断マニュアル、ENTONI No2"全日本病院出版会、編集企画夜陣紘治. 27-31 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 西野 宏: "耳鼻咽喉科診療プラクテイス 頭頸部腫瘍診療におけるdecision Making"分光堂、編集岸本誠司. 70-73 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 西野 宏, 市村恵一: "耳鼻咽喉科診療Q&A"六法出版社 名古屋. 486-487 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hiroshi Nishino: "Combined therapy with conservative surgery, radiotherapy, and regional chemotherapy for maxillary sinus carcinoma."Cancer. 89(9). 1925-1932 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takeharu Kanazawa: "Pleomorphic adenoma of the pterygopalatine fossa"Eur Arch otorhinolaryngol. 257. 433-435 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 西野宏: "切除不能な局所再発および転移頭頸部扁平上皮癌に対する化学療法"日耳鼻. 103(9. 963-969 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 西野宏: "喉頭早期声門癌放射線治療後再発に対する機能保存手術"頭頸部腫瘍. 26(3). 422-425 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 高橋克昌: "外切開により摘出した義歯による食道異物の1例."JOHNES. 16(4). 679-682 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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