IMMUNOLOGICAL RESPONSE IN IL-2 TRANSFECTED SQUAMOUS CELL CARCINOMA OF THE HEAD AND NECK
| Project/Area Number |
12671680
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | DOKKYO UNIVERSITY SCHOOL OF MEDICINE |
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Principal Investigator |
HIRABAYASHI Hideki DOKKYO UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF OTORHINOLARYNGOLOGY AND BRONCHOESOPHAGOLOGY, ASSOCIATE PROFESSOR, 医学部, 助教授 (00146185)
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| Co-Investigator(Kenkyū-buntansha) |
KANAYA Hiroaki DOKKYO UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF OTORHINOLARYNGOLOGY AND BRONCHOESPHAGOLOGY, ASSISTANT PROFESSOR, 医学部, 講師 (40265301)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | SQUAMOUS CELL CARCINOMA OF HEAD AND NECK / TUMOR SPECIFIC ANTIGEN / CYTOTOXIC T LYMPHOCYTE / 細胞障害性Tリンパ球 / 腫瘍抗原 / MHCクラスI分子 / セファロースゲルカラム / ELISPOT / 高速液体クロマトグラフィー |
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| Research Abstract |
We recently reported that cultured, IL-2 gene transfected squamous cell carcinoma of the head and neck had remarkable immunological response to lymphocytes. The aim of this study is to clarify the mechanism that how lymphocytes recognize tumor specific antigen and attack the tumor cells. We prepared large amount of cultured squamous cell carcinoma of the head nad neck, and lysed pelleted tumor cells for extraction of tumor antigen. Affinity purification was performed to purify MHC-bound peptide and fractionated by high-performance liquid chromatography for checking the response of lymphocytes. Mass spectrometric analysis showed a peak of molecular mass in fraction which lymphocytes responded. The peak of molecular mass was carefully estimated and sequences of tumor antigen were detected. In result 16 of antigen ( peptide ) were identified and synthesized. Induction of tumor specific lymphocytes was performed using dendritic cells and lymphocytes derived from healthy donor. Pulsed lymphocytes with dendritic cells and peptides were harvested and checked by ELISPOT assay. The incidence of 1/40 to 1/50 lymphocytes produce gamma-IFN which means lymphocytes responded against tumor cells. This suggests tumor specific lymphocytes were generated with our synthethized peptide of squamous cell carcinoma of the head and neck.
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Report
(4 results)
Research Products
(2 results)
