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Study of the neovasucular membranes in patients with age-related macular degeneration

Research Project

Project/Area Number 12671694
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionTohoku University

Principal Investigator

ABE Toshiaki  Ophthalmology, Tohoku Univ., Associate professor, 大学院・医学系研究科, 助教授 (90191858)

Co-Investigator(Kenkyū-buntansha) TOMITA Hiroshi  Ophthalmology, Tohoku Univ., Research Associate, 大学院・医学系研究科, 助手 (40302088)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2001: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2000: ¥2,500,000 (Direct Cost: ¥2,500,000)
Keywordsage-related macular degeneration / retinal pigment epithelium / cytokine / hypoxia / serum / VEGF / bFGF / TGFB / bFTGFβ / TGFβ
Research Abstract

Patients with age-related macular degeneration (AMD) generate variable degree of submacular hemorrhage, exudates, and retinal detachment and so on due to the submacular choroidal neovascularization (CNV). There have been no reports to treat the disease safely and steady and patients forced to be limited quality of life. One of the reasons is the unknown mechanism of the generation of the neovascular membranes. We examined the mRNA expression in the CNV from patients with AMD and compared them to those of other proliferative membranes from patients with proliferative vitreoretinopathy (PVR) and diabetic retinopathy (PDR). Statistically significant, expression of basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF) and transforming growth factor (TGF) beta was observed in the membrane of CNV when compared to those of PVR and PDR. bFGF expression increased with the enlargement of CNV, conversely VEGF and TGF beta expressed remarkably when the membranes were small size. We found bFGF and VEGF co-stimulated the enlargement of the CNV, conversely TGF beta inhibits the enlargement of the CNV using in vitro model of angiogenesis. We also found that hypoxia increase the expression of VEGF in the retinal pigment epithelium (RPE). The fact may be coincident with the previous report that hypo-perfusion may affect the generation of the CNV at the submacular lesions. Further, we examined the barrier function of the RPE using microporous filter support in the medium with several cytokines including VEGF and bFGF. The results revealed that VEGF might affect the barrier function of the RPE, but interleukin-1beta showed prominent effect for disrupting the function.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (37 results)

All Other

All Publications (37 results)

  • [Publications] Abe T, et al.: "Macular Degeneration Associated with Aberrant Expansion of Trinucleotide Repeat of SCA7 Gene in Two Japanese Families"Arch Ophthalmol. 118. 1415-1421 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Abe T, et al.: "Autologous iris pigment epithelial cell transplantion in monkey subretinal region"Curr Eye Res.. 20. 268-275 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] AbeT, Yoshida M, Kano T, Tamai M: "Visual function in the lesions after removal of subretinal neovascula membranes in patients with age-related macular degeneration"Graefe's Arch Clin Exp Ophthalmol. 239. 927-936 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Abe T, Abe K, Tsuda T, Itoyama Y., Tamai M: "Patients with spinocerebellar ataxia type 1 lack ophthalmological anticipation"Graefe's Arch Gli Exp Ophthalmol. 239. 722-728 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 阿部俊明: "眼科 NEW MOOK"金原出版. 153-158 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Abe T, Yoshida M, Kano T, Tamai M: "Visual function in the lesions after removal of subretinal neovascular membranes in patients with age-related macular degeneration"Graefe's Arch Clin Exp Ophthalmol. 239. 927-936 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Abe T, Abe K, Tsuda T, Itoyama Y, Tamai M: "Patients with spinocerebellar ataxia type I lack ophthalmological anticipation"Graefe's Arch Clin Exp Ophthalmol. 239. 722-728 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Udono T, Takahashi K, Yasumoto K, Yoshizawa M, Takeda K, Abe T, Tamai M, Shibahara S: "Expression of tyrosinase-related protein 2/DOPAchrome tautomerase in retinoblastoma"Exp Eye Res. 72. 225-34 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Wada Y, Abe T, Sato H, Tamai M: "A novel Gly35Ser mutation in the RDH5 gene in a Japanese family with fundus albipunctatus associated with cone-dystrophy"Arch Ophthalmol. 119. 1059-63 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Wada Y, Abe T, Takeshita T, Sato H, Tamai M: "Mutation of human retinal fascin gene (FSCN2) causes autosomal dominant retinitis pigmentosa"Invest Ophthalmol Vis Sci. 42. 2395-400 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sato M, Abe T, Tamai M: "Expression of the Varicella Zoster Virus Thymidine Kinase and Cytokines in Patients with Acute Retinal Necrosis Syndrome"Jpn J Ophthalmol. 1 ; 44. 693 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Motoko Sakuragi, Hiroshi Tomita, Toshiaki Abe, Makoto Tamai: "Changes of phagocytic capacity in basic fibroblast growth factor-transfected iris pigment epitheclial cells in rats"Curr eye res. 23. 185-191 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Suzuki T, Takahashi K, Kuwahara S, Wada Y, Abe T, Tamai M: "A novel (Pro79Thr) mutation in the FKHL7 gene in a Japanese family with Axenfeld-Rieger syndrome"Am J Ophthalmol. 132. 572-575 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshida M, Abe T, Kano T, Tamai M: "Two types of optical coherence tomographic images of retinal pigment epithelial detachments with different prognosis"BJO. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nishikatsu H, Abe T, Moriya T, Sato E, Tamai M: "Conjunctival malignant melanoma with iris nevus and dysgenesis of corpus callosum"Ann Ophthalmol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sato M, Abe T, Tamai M: "Analysis of the metallothionein gene in age-related macular degeneration"Jpn. J. Ophthalmol. 44. 115-121 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Wada Y, Nakazawa M, Abe T, Tamai M: "A new Leu253Arg mutation in the RP2 gene in a Japanese family with X-linked retinitis pigmentosa"Invest Ophthalmol Vis Sci. 41. 290-293 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ren G, Fuse N, Abe T, Tamai M: "mRNA expression of proto-oncogenes and platelet-derived growth factor in proliferative vitreoretinal disease"Jpn. J. Ophthalmol. 44. 308-11 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Abe T, Sato M, Tamai M: "Variable R1 region in Varicella-zoster virus was diverse in a fulminant type of acute retinal necrosis syndrome"Br. J. Ophthalmol. 84. 193-198 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fuse N, Suzuki T, Wada Y, Yoshida M, Abe T, Nakazawa M, Tamai M: "Molecular genetic analysis of ABCR gene in Japanese dry-type age-related macular degeneration"JJO 2000. 44. 245-9

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Udono T, Totsune K, Takahashi K, Abe T, Sato M, Shibahara S, Tamai M: "Increased expression of adrenomedullin mRNA in the tissues of intra and orbital tumors"Am J Ophthalmol. 129. 555-556 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Wada Y, Abe T, Fuse N, Tamai M: "A frequent 1085 del C/insCAAG mutation in the RDH5 gene of the Japanese patients with fundus albipunctatus"Invst. Ophthalmol. Vis Sci. 41. 290-293 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Abe T, Tomita H, Kano T, Yoshida M, Nakamura Y, Nishikawa S, Tamai M: "Autologous iris pigment epithelial cell trasplantation in monkey subretinal region"Curr Eye Res. 20. 268-275 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakazawa T, Abe T, Sato Y, Yuda F, Omura M: "Magnetic resonance imaging of a non-pigmented adenoma of the ciliary epithelium"Acta Ophthalmol. Scand. 78. 470-473 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Abe T, Tsuda T, Yoshida M, Wada Y, Itoyama Y, Tamai M: "Macular Degeneration Associated with Aberrant Expansion of Trinucleotide Repeat of SCA7 Gene in Two Japanese Families"Arch Ophthalmol. 118. 1415-1421 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tomita H, Abe T, Tamai M: "Ceramide-induced cell death in cultured rat retinal pigment epithelial cells"Tohoku J. Exp. Med.. 190. 223-229 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kunikata H, Abe T, Tamai M: "The characteristics of congenital hypertrophy of retinal pigment epithelium in Turcot syndrome"Ophthalmologica. 214. 374-5 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Abe T, Yoshida M, Tomita H, Kano T, Sato M, Wada Y, Fuse N, Yamada T, Tamai M: "Auto Iris Pigment Epithelial Cell Transplantation in Patients with Age-related Macular Degeneration : Short-term results"Tohoku J Exp. Med.. 191. 7-20 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Abe T, Yoshida M, Kano T, Tamai M: "Visual function in the lesions after removal of subretinal neovascular membranes in patients with age-related macular degeneration"Graefe's Arch Clin Exp Ophthalmol. 239. 927-936 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Abe T, Abe K, Tsuda T, Itoyama Y, and Tamai M: "Patients with spinocerebellar ataxia type 1 lack ophthalmological anticipation"Graefe's Arch Clin Exp Ophthalmol. 239. 722-728 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 阿部俊明: "眼の創傷治癒、色素細胞移植の細胞生物学"眼科 NEW MOOK. 1. 153-158 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 阿部俊明: "移植再生医療の可能性"眼科. 43. 1697-1702 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 阿部俊明: "加齢黄斑変性に対する網膜色素上皮移植"眼科診療レスキュー. 4. 126-127 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 阿部俊明: "ぶどう膜炎、電気生理学的所見"眼科診療プラクティス. 68. 64-65 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Abe T, et al.: "Macular Degeneration Associated with Aberrant Expansion of Trinucleotide Repeat of SCA7 Gene in Two Japanese Families."Arch Ophthalmol. 118. 1415-1421 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Abe T, et al.: "Autologous iris pigment epithelial cell transplantation in monkey subretinal region."Curr Eye Res.. 20. 268-275 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 阿部俊明: "加齢黄斑変性に対する網膜色素上皮移植"メディカルビュー社. 278-279 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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