| Project/Area Number |
12671697
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | CHIBA UNIVERSITY |
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Principal Investigator |
NOROSE Kazumi Chiba University, Graduate School of Medicine, Assistant, 大学院・医学研究院, 助手 (30156244)
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| Co-Investigator(Kenkyū-buntansha) |
IWAKURA Yoichiro University of Tokyo, Institute of Medical Science, Center for Experimental Medicine, 医科学研究所, 教授 (10089120)
YANO Akihiko Chiba University, Graduate School of Medicine, Professor, 大学院・医学研究院, 教授 (20135122)
AOSAI Fumie Chiba University, Graduate School of Medicine, Associate Professor, 大学院・医学研究院, 助教授 (80150316)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Toxoplasma / chorioretinitis / heat shock protein / interferon gamma / organ specificity / interferon gamma knock out mouse |
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| Research Abstract |
1. To determine the influence of interferon (IFN)-γ on the organ infectivity, on the Toxoplasmic chorioretinitis, and on the genetic susceptibility of susceptible (C57BL/6) and resistant (BALB/c) strains after peroral infection with cystis of Toxoplasma gondii. IFN-γ knockout (KO) mice in C57BL/6 and BALB/c backbrounds were utilized. The kinetics of the changes in T.gondii abundance were evaluated with a quantitative competitive polymerase chain reaction assay in various organs including eyes at different times after peroral infection. In IFN-γ KO mice, a T.gondii-specific gene, SAG1, was detected in all organs examined, and the protozoan proliferated much more actively than in wild-type mice. The abundance of T.gondii was much higher in mesenteric lymph nodes and the heart than in other organs. In contrast, in the nervous system organs and kidneys, only a weakly detectable reaction was observed. Toxoplasma gondii grew at a more rapid rate in the organs of IFN-γ KO C57BL/6 mice than in the organs of IFN-γ KO BALB/c mice during the course of infection. Destruction of the INF-γ showed remarkable effects on the infectivity in both susceptible and resistant mice.
2. The high-level expression of gondii HSP70 was correlated with mortality in IFN-γ KO mice infected with T.gondii.
3. SAG1 was detected in the eye of IFN-γ KO mice infected with T.gondii perollary.
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