Role of earlyocular chemokines and chemokine receptors after corneal allograft

• Project/Area Number: 12671720
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Jichi Medical
• Principal Investigator: YAMAGAMI Satoru Jichi Medical Schl., Assistant. Prof., 医学部, 講師 (10220245)
• Co-Investigator(Kenkyū-buntansha): TSURU Tadahiko Jichi Medical Sch., Professor, 医学部, 教授 (90126128) ; SATAI Rieko Jichi Medical Sch., Assistant.Prof., 医学部, 助手 (20316540) ; OHKAWA Taeko Jichi Medical Sch., Assistant.Prof., 医学部, 助手 (80306117)
• Project Period (FY): 2000 – 2001
• Project Status: Completed (Fiscal Year 2001)
• Budget Amount: ¥3,400,000 (Direct Cost: ¥3,400,000); Fiscal Year 2001: ¥1,600,000 (Direct Cost: ¥1,600,000); Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: corneal transplantation / chemokine / cervical lymph nodes / chemokine Receptor / allograft rejection / 頸部リンパ節 / ランゲルハンス細胞 / CCR5 / 角膜 / ノックアウトマウス

Research Abstract

We investigated chemokines and chemokine receptors in corneal Langerhans cell migration and allograft rejection. We showed the over expression of RANTES, MIP-1α, MIP-1β, MCP-1 and their receptors such as CCR1, CCR2 and CCR5 mRNAs in the postoperative early as well as effectors phases of corneal transplants. Moreover, CCR1 play a critical role in generating allograft rejection by performing knockout mouse study. In Langerhans cell migration of corneal epithelium after cauterization ; CCR5 functionally affects Langerhans cell migration in cornea. These results suggest that chemokine and their receptors are closely associated with the cell recruitment in allo-recognition and allo-sensitization after corneal transplants. In addition, we studied critical role of draining cervical lymph nodes in corneal allograft rejection. The study to reveal correlation between chemokine and cervical lymph nodes/corneas is on going in our laboratory.

Research Products

• [Publications] Yamagami S: "The critical role of draining lymph nodes in corneal alloimmunization and graft rejection"Invest Ophthalmol Vis Sci. 42. 1293-1298 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Qian Y: "Topical soluble tumor necrosis factor receptor type I suppresses ocular chemokine gene expression and rejection of allogeneic corneal transplants"Arch Ophthalmol. 118. 1666-1671 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yamagami S: "Draining lymph nodes play an essential role in alloimmunity generated in response to high-risk corneal transplantation"Cornea. (in press).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yamagami S, et al: "The critical role of lymph nodes in corneal allo-immunigation and graft rejection"Invest ophthalmol Vis Sci. 42. 1293-1296 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Qian Y et al: "Topical soluble tumor necrosis factor alpha receptor type I suppresses ocular chemokine gene expression and rejection of allogeneic corneal transplants"Arch Ophthalmol. 118. 1666-1671 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yamagami S, et al: "Draining lymph nodes play an essential role in allo immunity generated in response to high-risk corneal transplantation"Cornea. (in press).
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yamagami S: "The critical role of lymph nodes in corneal allo-immunization and graft rejection"Invest Ophthalmol Vis Sci. 42. 1293-1298 (2001)
• [Publications] Qian Y: "Topical soluble tumor necrosis factor alpha receptor type I suppresses ocular chemokine gene expression and rejection of allogeneic corneal transplants"Arch Ophthalmol. 118. 1666-1671 (2001)
• [Publications] Yamagami S: "Draining lymph nodes play an essential role in alloimmunity generated in response to high-risk corneal transplantation"Cornea. (in press).