| Project/Area Number |
12671720
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Jichi Medical |
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Principal Investigator |
YAMAGAMI Satoru Jichi Medical Schl., Assistant. Prof., 医学部, 講師 (10220245)
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| Co-Investigator(Kenkyū-buntansha) |
TSURU Tadahiko Jichi Medical Sch., Professor, 医学部, 教授 (90126128)
SATAI Rieko Jichi Medical Sch., Assistant.Prof., 医学部, 助手 (20316540)
OHKAWA Taeko Jichi Medical Sch., Assistant.Prof., 医学部, 助手 (80306117)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | corneal transplantation / chemokine / cervical lymph nodes / chemokine Receptor / allograft rejection / 頸部リンパ節 / ランゲルハンス細胞 / CCR5 / 角膜 / ノックアウトマウス |
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| Research Abstract |
We investigated chemokines and chemokine receptors in corneal Langerhans cell migration and allograft rejection. We showed the over expression of RANTES, MIP-1α, MIP-1β, MCP-1 and their receptors such as CCR1, CCR2 and CCR5 mRNAs in the postoperative early as well as effectors phases of corneal transplants. Moreover, CCR1 play a critical role in generating allograft rejection by performing knockout mouse study. In Langerhans cell migration of corneal epithelium after cauterization ; CCR5 functionally affects Langerhans cell migration in cornea. These results suggest that chemokine and their receptors are closely associated with the cell recruitment in allo-recognition and allo-sensitization after corneal transplants. In addition, we studied critical role of draining cervical lymph nodes in corneal allograft rejection. The study to reveal correlation between chemokine and cervical lymph nodes/corneas is on going in our laboratory.
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