• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Improvement and estimation of extra dellular matrix for proloferative vitreoretinopathy

Research Project

Project/Area Number 12671729
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionKansai Medical University

Principal Investigator

MATSUMURA Miyo  Kansai Medical University Department of Ophthalmology Progessor, 医学部, 教授 (30144380)

Co-Investigator(Kenkyū-buntansha) OOMORI Kyouko  Kansai Medical University, Associate Professor, 医学部, 助教授 (90152256)
YUGE Kenshi  Kansai Medical University, Instructor, 医学部, 助手 (10247942)
NISHIMURA Tetsuya  Kansai Medical University, Associate Professor, 医学部, 助教授 (30156111)
FUJISEKI Yoshito  Kansai Medical University, Instructor, 医学部, 助手 (30330202)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordsproliferative vitreoretinopathy(PVR) / Lysyl oxidase / collagen / retinal pigment epithelium / migreatin / 硝子体 / 硝子体手術 / 内境界膜剥離 / ICG染色
Research Abstract

Lysyl oxidase (LO) is a catalyst of crosslinkage between the collagen which is extracellular matrix (ECM) consitution ingredient. We recognized expression of the LO MRNA was very high in a cultured retinal pigment epithelium cell (RPE) of albino rabbit. We examined the regulation ofthe expression in relation to the factors which change proliferation and differentiation of the cell in order to reseach r role of this mRNA.
Methods : It wsa known that RPE cells of 3〜4 passage continue to grow to confluent on dish, but the proliferation was inhibited and the cells showed the cobble stone structure like in vivo under the presence of retinoic acd-cAMP. We investigated the reguletion of expression of LO mRNA using the three kind of cultured RPE and control cells.
Results : 1.When RPE cells showed differenciation on the presence of retinoic acid or retinoic acid cAMP, the expression of LO mRNA was decreased and α-N-acetylglucosaminidase(NAG) which was a marker enzyme of lysozome was increased. 2.The cells which were cultured on micropore membrane showed low-expression of LO mRNA and high NAG mRNA expression significntly compared with the cells cultured on dishes.
Conclusions : It was suggested that RPE cells proliferate buiding up ECM via the expression of LO, and the cells in the differenciated stage have more mobility and low ability of ECM formation. LO may contribute to conversion of the RPE cells from differenciated type to proliferating form, which would iniciate the severe form of retial detachment ; proliferative vitreoretinopathy(PVR). For development of new method of vitrectomy for PVR which is occurred by RPE cell proliferation and ECM formation, thise findings of RPE cells will make useful contributions.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Ogata N, et al.: "Expression of cytokines and transcription factors in photocoagulated human retinal pigment epithelial cells"Graefe's Arch.Clin.Exp.Ophthalinol. 239. 87-95 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ogata N, et al.: "Pigment epithelium derived factor as a neuroprotective agent against ischernic retinal injury"Current Eye Research. 22. 245-252 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ogata N, et al.: "Pigment epithelium-derived factor in the vitreous is low in diabetic retinopathy and high inrhgmatogenous retinal detachment"Am J ophthalmol. 132. 378-382 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ogata N, et al.: "Upregulation of pigment epithelium-derived factor after laser photocoagulation"Am J Ophthalmol. 132. 427-729 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 藤関義人 他: "硝子体手術を行った nanophthalmos を伴う uveal effusion syndrome"臨床眼科. 55. 787-791 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 山田晴彦 他: "多発性後極部網膜色素上皮症の光干渉断層計所見"臨床眼科. 55. 850-853 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 松村美代: "黄斑下手術"医学書院、東京. 127-135 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ogata N, et al: "Expression of cytokines and transcription factors in photocoagulated human retinal piment epithelial cells."Graefe's Arch clin Exp Ophthalmol. 239. 87-95 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ogata N, et al: "Pigment epithalium derived factor as a neuroprotective agent against ischemic retinal injury."Current Eye Research. 22. 245-252 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ogata N, et al: "Pigment epithalium derived factor in the vitreous is low in diabetic retinopathy and high inrhgmatogenous retinal detachment."Am J Ophthalmol. 132. 378-382 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ogata N, et al: "Upregulation of pigment epithelium derived factor after laser photocoagulation."Am J Ophthalmol. 132. 427-429 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fujiseki Y, et al: "Two cases of nanophthalmic uveal effusion syndrome treated by vitrectomy."Rinshoganka. 55. 787-791 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamada H, et al: "Optical coherence tomographic features of multiple posterior pigment epitheliopathy."Rinshoganka. 55. 850-853 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ogata N, et al.: "Expression of cytokines and transcription factors in photocoagulated human retinal pigment epithelial cells"Graefe's Arch clin Exp Ophthalmol. 239. 87-95 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ogata N, et al.: "Pigment epithelium derived factor as a neuroprotective agent against ischemic retinal injury"Current Eye Research. 22. 245-252 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ogata N, et al.: "Pigment epithelium-derived factor in the vitreous is low in diabetic retinopathy and high inrhgmatogenous retinal detachment"Am J ophthalmol. 132. 378-382 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ogata N, et al.: "Upregulation of pigment epithelium-derived factor after laser photocoagulation"Am J Ophthalmol. 132. 427-429 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 藤関義人 他: "硝子体手術を行ったnanophthalmosを伴うuveal effusion syndrome"臨床眼科. 55. 787-791 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 山田晴彦 他: "多発性後極部網膜色素上皮症の光干渉断層計所見"臨床眼科. 55. 850-853 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 松村美代: "黄斑下手術"医学書院、東京. 127-135 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Otani A,Matsumura M, et al: "Angiotensin II - stimulated vascular endothelial growth factor expression in bovine retinal pericytes."Invest Ophthalmol Vis Sci.. 41. 1192-1199 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Mizoguchi T,Matsumura M, et al: "Surgical effects of combined trabeculotomy and sinusotomy compared to trabeculotomy alone."Acta Ophthalmol Scand.. 78. 191-195 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 石郷岡均,松村美代 他: "黄斑円孔硝子体手術後の視野欠損に対する予防対策"眼科臨床医報. 94. 1244-1247 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 杉本琢二,松村美代 他: "虹彩隅角新生血管を伴わない増殖糖尿病網膜症に対する硝子体手術後の長期経過"臨床眼科. 54. 1725-1729 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 西信昭子,藤関義人,松村美代 他: "白点状眼底に生じた血管新生黄斑症"臨床眼科. 54. 1772-1774 (2000)

    • Related Report
      2000 Annual Research Report

URL: 

Published: 2000-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi