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Roles of Extracellular Matrix in Developmental Mechanism and Treatment of Glaucoma

Research Project

Project/Area Number 12671732
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionUniversity of Occupational and Environmental Health

Principal Investigator

TAWARA Akihiko  University of Occupational and Environmental Health, Faculty of Medicine, Professor, 医学部, 教授 (90117169)

Co-Investigator(Kenkyū-buntansha) NISHIO Youko  University of Occupational and Environmental Health, Faculty of Medicine, Assistant Professor, 医学部, 助手 (70322609)
HIROSE Naofumi  University of Occupational and Environmental Health, Faculty of Medicine, Assistant Professor, 医学部, 助手 (20258619)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2002: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsextracellular matrix / MYOC protein / steroid-induced glaucoma / immunohistochemistry / nipradilol / MMPs / TIMPs / type VI collagen / MMP / TIMP / アクアポリン / 血管新生緑内障 / 虹彩ルベオーシス / VI型コラーゲン / 原発開放隅角緑内障
Research Abstract

1. We examined immunohistochemically expression of the extracellular matrixes and their metabolizing enzymes in the monkey eyes that were injected triamcinolone acetonide under the tenon capsule to study the effect of corticosteroid on the extracellular matrix in the trabecular meshwork. The results suggested that corticosteroid could increase the amount of MYOC protein in the trabecular meshwork, and that it also could increase expression of TIMP-2 in the trabecular meshwork and MMP-3, MMP-9 and TIMP-2 in the ciliary body.
2. We immunohistochemically stained the extracellular matrixes in the trabecular meshwork obtained by trabeculectomy from a patient with corticosteroid-induced glaucoma. The results indicated that MYOC protein might increase in the glaucomatous cornea but not in the trabecular meshwork. The results also showed that both heparan sulphate-type proteoglycan and type IV collagen increase in the trabecular meshwork. With double staining, MYOC protein was co-localized with … More type VI collagen in the tissue.
3. We examined immunohistochemically localization of MMPs and TIMPs in the normal rat eyes. The results indicated that MMP-9 and TIMP-1 are localized in the cornea, MMP-9 and TIMP-2 are in the trabecular meshwork, and MMP-9, TIMP-1 and TIMP-3 are in the ciliary body.
4. We examined immunohistochemically the effect of anti-glaucoma drug of nipradilol, both α- and β-blocker, on MMPs and TIMPs in rat eyes. From this study, it is indicated that nipradilol increases the amount of MMP-2 and MMP-9, and TIMP-1, and decreases TIMP-2 in the trabecular meshwork.
5. We examined histologically, immunohistochemically and with in situ hybridization the trabecular tissue from rabbit with experimental anterior segment ischemia to investigate the mechanism of neovascularization in the anterior segment of the eye. The results suggested that vascular endothelial growth factor (VEGF) plays an important role in development of neovascularization in the anterior segment of the eye. Less

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Tawara, A.: "Immunohistochemical localization of MYOC/TIGR protein in the trabecular tissue of normal and glaucomatous eyes"Curr. Eye Res.. 21-6. 934-943 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 田中 拓司: "チモロール静脈内投与の家兎脈絡膜血流に及ぼす影響"あたらしい眼科. 18-3. 385-389 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 永谷 建: "正常眼および正常眼圧緑内障眼における視神経乳頭と脈絡膜の循環"眼科臨床医報. 95-11. 1109-1113 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tawara, A.: "Neovascularization in the anterior segment of the rabbit eye by experimental anterior ischemia"Graefe's Arch Clin Exp Ophthalmol. 240-2. 144-153 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 廣瀬 直文: "ラタノプロスト点眼が調節の準静的特性に及ぼす影響"臨床眼科. 56-6. 957-960 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 廣瀬 直文: "注射針による線維柱帯切除術後の濾過胞再建術"眼科手術. 16-2. 207-211 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 田原 昭彦: "眼の細胞生物学"中山書店. 323 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tawara A., Okada Y., Kubota T., Suzuki Y., et al.: "Immunohistochemical localization of MYOC/TIGR protein in the trabecular tissue of normal and glaucomatous eyes"Curr Eye Res. 21・6. 934-943 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tanaka T., Tawara A., Tsujioka K., Sudo Y., et al.: "Effects of timolol on choroidal blood flow in rabbits"Journal of the Eye. 18・3. 385-389 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nagaya K., Tawara A., Takahashi H., Fujii H.: "Microcirculation in the optic nerve head and the choroid in eyes with or without normal-tension glaucoma"Jpn Rev Clin Ophthalmol. 95・11. 1109-1113 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tawara A., Kubota T., Hata Y., Sakamoto T., et al.: "Neovascularization in the anterior segment of the rabbit eye by experimental anterior ischemia"Graefe's Arch Clin Exp Ophthalmol. 240・2. 144-153 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirose N., Iwasaki T., Nasu T., Ibi K., Tawara A.: "Effect of a signal instillation of latanoprost on the quasi-static characteristics of accommodation"Jpn J Clin Ophthalmol. 56・6. 957-960 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirose N., Harada Y., Nasu T., Tanaka T., Ibi K., Tawara A.: "Transconjunctival needle revision of filtering blebs after failed trabeculectomy"Jpn J Ophthalmic Surg. 16・2. 207-211 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirose N., Nasu T., Harada Y., Tanaka T., Tawara A.: "Excisional revision of failed trabeculectomy blebs"Jpn J Ophthalmic Surg. 16・2. 239-242 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tawara A.: "Eye and extracellular matrix, in Ocular Cell Biology"Nakayama Shoten. 8 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tawara A.: "Primary open angle glaucoma, in Ocular Cell Biology"Nakayama Shoten. 4 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tawara A., Kubota T., Hata Y., Sakamoto T., et al.: "Neovascularization in the Anterior Segment of the Rabbit Eye by Experimental Anterior Ischemia"Graefe's Arch Clin Exp Ophthalmo. 240・2. 144-153 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tawara A., Kubota T., Hata Y., Sakamoto T.et al.: "Neovascularization in the Anterior Segment of the Rabbit Eye by Experimental Anterior Ischemia"Graefe's Arch Clin Exp Ophtalmol. (印刷中).

    • Related Report
      2001 Annual Research Report
  • [Publications] Tawara A.,Okada Y.,Kubota T.,Suzuki Y. et al.: "Immunohistochemical Localization of MYOC/TIGR Protein in the Trabecular Tissue of Normal and Glaucomatous Eyes"Curr.Eye Res.. (印刷中).

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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