| Project/Area Number |
12671734
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatric surgery
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| Research Institution | Akita University |
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Principal Investigator |
HEBIGUCHI Tatsuzo AKITA UNIVERSITY SCHOOLOF MEDICINE ASSOCIATE PROFESSOR, 医学部, 助教授 (00124644)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHINO Hiroaki AKITA UNIVERSITY SCHOOL OF MEDICINE RESEARCH ASSOCIATE, 医学部, 助手 (90182807)
KATO Tetsuo AKITA UNIVERSITY SCHOOL OF MEDICINE PROFESSOR, 医学部, 教授 (20004963)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | short small bowel / renal tubulointerstitial fibrosis / nitric oxide (NO) / arginine deficiency / citrulline / microdialysis / focal tubulointerstitial fibrosis |
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| Research Abstract |
Background and Aim : It is known that arginine (Arg) becomes an essential amino acid after massive resection of the small bowel due to decreased biosynthesis of citrulline (Cit) in the remaining small bowel. It is also reported that nitric oxide (NO) is synthesized from L-Arg by NO synthase (NOS), and NO is involved in the regulation of blood flow in the kidney. We experienced a patient with an extremely short small bowel, showing tubulointerstitial fibrosis (TIF), The experiment was designed to clarify whether massive small bowel resection produces TIF in the kidney.
Materials and Methods: An experimental study was performed using 4-week-old rats with 90% proximal small bowel resection (90% SBR) either with or without Arg supplementation for 6 weeks after surgery.
Results: In rats without Arg supplementation, low plasma levels of Cit and Arg, increased urinary excretion of orotate, and TIP was observed 6 weeks after 90% SBR. The data from plasma aminogram, and increased excretion of urinary orotate suggested the presence of Arg deficiency. Rats with Arg supplementation after 90% SBR, and pair-fed control rats without 90%SBR demonstrated almost normal glomeruli and tubulointerstitium. It is suggested that NO production in group 1 is maintained as well as control groups by analyzes of Arg, Cit and NO x (N02^-/N03^-) in diaiysates of renal parenchyma using microdialysis technique.
Conclusion: Experimental study strongly suggests that Arg deficiency causes TIF in the kidney after massive small bowel resection. Arginine prevents renal tubulointerstitium from fibrosis after massive small bowel resection.
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