| Project/Area Number |
12671744
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Pediatric surgery
|
|---|
| Research Institution | JUNTENDO UNIVERSITY |
|---|
Principal Investigator |
YAMATAKA atsuyuki JUNTENDO UNIVERSITY SCHOOL OF MEDICINE Associate PROFESSOR, 医学部, 助教授 (40200703)
|
|---|
| Project Period (FY) |
2000 – 2002
|
| Project Status |
Completed (Fiscal Year 2002)
|
| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2002: ¥100,000 (Direct Cost: ¥100,000)
Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
|
|---|
| Keywords | Bladder / Transplantation / Omentum / Rat / Augmentation / Augumentation / 膀胱 / 移植 / 膀胱拡大術 / 免疫寛容 / 拒絶反応 |
|---|
| Research Abstract |
In patients with bladder or cloacal exstrophy, or neurogenic bladder a surgical procedure that is conventionally done to increase bladder capacity is bladder augmentation Ileum, colon or stomach is usually used but permanent contact of urine with intestinal or gastric mucosa is not free from complications and there is a risk of malignancy. In an attempt to prevent such nonphysiological interaction we performed bladder transplantation to create viable bladder tissue that may be used for bladder augmentation.
The bladder of new born BN rats (donor) was excised and each was transplanted into a pouch created in the distal omentum of a 5-week-old Lewis rats (recipient), Bladder augmentation was performed by anastomosing the graft to the recipient bladder 10 days after transplantation. FK506 was used as a immunosuppressant.
Results : 1) FK506 can prevent rejection in the allogeneic bladder transplantation in rats. 2) Our technique has a potential for creating viable bladder tissue that may be used for bladder augmentation.
Using 16-week-old adult rats as a donor, bladder transplantation and augmentation was performed in the same procedure. Results were compared with standard ileocystplasty.
Results : 3) Bladder transplantation group has better function with less complications than ileocystplasty group at 16 months after either type of augmentation. 4) Adult rat's bladder also can be used for the bladder transplantation in our model. (living-related transplantation)
|
