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Mutational events of p53 gene in salivary gland tumors

Research Project

Project/Area Number 12671766
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionNiigata University

Principal Investigator

CHENG Jun  Graduate School of Medical and Dental Sciences, Niigata University, Associate Professor, 大学院・医歯学総合研究科, 助教授 (40207460)

Co-Investigator(Kenkyū-buntansha) IDA-YONEMOCHI Hiroko  Graduate School of Medical and Dental Sciences, Niigata University, Assistant, 大学院・医歯学総合研究科, 助手 (60293213)
SAKU Takashi  Graduate School of Medical and Dental Sciences, Niigata University, Professor, 大学院・医歯学総合研究科, 教授 (40145264)
OHSHIRO Kazufumi  Graduate School of Medical and Dental Sciences, Niigata University, Assistant, 大学院・医歯学総合研究科, 助手 (50332648)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2000: ¥2,500,000 (Direct Cost: ¥2,500,000)
Keywordssalivary gland tumor / p53 / cancer suppressor gene / gene mutation / DNA / PCR / sequecing / SSCP / 血管分布
Research Abstract

In order to determine mutational events of the p53 tumor suppressor gene in salivary gland tumors, 117 cases of surgical materials of salivary tumors were analyzed by a direct sequencing of PCR products of DNA samples extracted from formalin-fixed paraffin-embedded tissue sections. The samples included 35 cases of lymphoepithelioma, 30 of mucoepidermoid carcinoma, 33 of Warthin tumor and 19 of pleomorphic adenoma. At the same time, cultured cells of adenoid cystic carcinoma were also examined.
Lymphoepithelioma is an undifferentiated carcinoma with the lymphoid stroma and associated with the Epstein-Barr virus infection. The pathogeneses of mucoepidermoid carcinomas and Warthin tumors have been related to the exposure to ionizing radiation by atomic bomb in Hiroshima and Nagasaki. However, nothing is known about the pathogenesis for other types of salivary gland tumor. The investigation was performed in the background of the so far accumulated information.
In this study we first examined … More exons 5-7 of p53 gene with PCR-fluoro-SSCP and then cases with abnormal SSCP profiles were subjected to direct sequencing. The results were compared with immunohistochemical staining patterns for p53 protein. Most conspicuous mutational events of p53 gens were found in exons 5-7 of lymphoepitheliomas, which instead showed scarce p53 protein immunopositivity. These mutational points were highly common among the cases examined. In contrast, no apparent mutational events were detected in pleomorphic adenomas, even in those with atypical cells that showed strong immunoreactions for p53 gene products. Warthin tumors and mucoepidermoid carcinomas often showed several point mutations in exons 5 and 7, which did not effect on amino acid translation so much, which seemed to be resulted from genetic polymorphism of the gene. However, these point mutations were shared by many cases of malignant and benign salivary gland tumors.
These results suggest that the specific point mutations in exons 5 and 7 of the p53 gene found in the present study are important genetic background for salivary gland tumorigenesis. Less

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Saku T: "Epstein-Barr virus infected lymphoepithelial carcinomas of the salivary gland in the Russia-Asian area : a clinicopathologic study of 160 cases"Arch Pathol. 47(1). 45-51 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohtake S: "Precancerous foci in pleomorphic adenoma of the salivary gland : recognition of focal carcinoma and atypical tumor cells by P53 immunohistochemistry"J Oral Pathol Med. 31(10). 590-597 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Maruyama S: "Sebaceous lymphoadenoma of the lip"J Oral Pathol Med. 31(4). 242-243 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Jen KY: "Mucoepidermoid carcinoma in children"Oral Med Pathol. 7(1). 27-31 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Cheng J: "p53 mutations in salivary gland tumors"5^<th> Joint conference of the American Association for Cancer Research and the Japanese Cancer Association, Maui, Hawaii, Feb.12-16. Abstract. B-67 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Cheng J: "Biological background for differential diagnosis of salivary pleomorphic adenoma from myoepithelioma by CT images and characteristic histopathology of paucivascular stroma"2^<nd> International Symposium of Molecular Pathology, Chengdu, China, Aug.18-23. Abstract. 50 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Saku T, Chang J, Jen KY et al.: "Epstein-Barr virus infected lymphoepithelial carcinomas of the salivary gland in the Russia-Asian area: a clinicopathologic study of 160 cases"Arch Pathol. 47(1). 45-51 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohtake S, Cheng J, Ida H, Suzuki M, Ohshiro K, Zhang W and Saku T: "Precancerous foci in pleomorphic adenoma of the salivary gland: recognition of focal carcinoma and atypical tumor cells by P53 immunohistochemistry"J Oral Pathol Med. 31(10). 590-597 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Maruyama S, Cheng J, Inoue T, Takagi R and Saku T: "Sebaceous lymphoadenoma of lip"J Oral Pathol Med. 31(4). 242-243 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Jen KY, Cheng J, Maruyama S, Hayashi T, Suzuki I, Shigaki S and Saku T: "Mucoepidermoid carcinoma in children"Oral Med Pathol. 7(1). 27-31 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Cheng J, Abe M, Saku T: "p53 mutations in salivary gland tumors"5^<th> Joint Conference of the American Association for Cancer Research and the Japanese Cancer Association, Maui, Hawaii, Feb. 12-16, Abstract. 9 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Cheng J, Maruyama S, Hayashi T, Wu L, Zhou Z and Saku T: "Biological background for differential diagnosis of salivary pleomorphic adenoma from myoepithelioma by CT images and characteristic histopathology of paucivascular stroma"2^<nd> International Symposium of Molecular Pathology, Chengdu, China, Aug. 18-23, Abstract. 50 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Cheng, J. et al.: "p53 mutaions in salivary gland tumors"5th Joint conference of the American Associations for Cancer Research and the Japanese Cancer Association. (Abstract). B-67 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Jen, K. et al.: "Mucoepidermoid carcinoma of children"Oral Medicine & Pathology. 6(2). 111 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Cheng, J. et al.: "Biological background for differential diagnosis of salivary pleomorphic adenoma from myoepithelioma by CT images and characteristic histopatholgy of paucivascular stroma"Second International Symposium of Molecular Pathology. (Abstract). 50 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 程 くんほか: "唾液筋上皮腫の臨床病理学的特徴"日本病理学会会誌. 90(1). 340 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Cheng, J. et al.: "Vascular distribution of salivary gland tumors refleted into their histology"First International Congress on Salivary Gland Diseases. (Abstract). 90 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Cheng,J.: "p53 mutations in salivary gland tumors"5^<th> Joint conference of the American Association for Cancer Research and the Japanese Cancer Association, Maui,Hawaii,Feb.12-16. Abstract. B-67 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kimura,S.: "Perlecan (heparan sulfate proteoglycan) gene expression reflected in the characteristic histological architecture of salivary adenoid cystic carcinoma."Virchows Arch. 437(2). 122-128 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 阿部真由美: "放射線関連唾液腺腫瘍におけるp53遺伝子変異"新潟歯学会雑誌. 30(2). 272 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 程くん: "唾液腺炎の導管再生における基底膜ヘパラン硫酸プロテオグリカンの役割"日本病理学会会誌. 89(1). 326 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 藤森行彦: "唾液腺腺様嚢胞癌における偽嚢胞の成立機序-基底膜関連分子産生担当細胞の同定-"日本病理学会会誌. 89(1). 252 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 丸山智: "唾液腺多形性腺腫の被膜浸潤"歯科基礎医学会雑誌. 42(5). 436 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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