| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2002: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Research Abstract |
Although retinoic acid was reported to compromise desmosome expression in the human epidermis through downregulation of desmosomal cadherins, it is unclear whether retinoic acid inhibits the formation of desmosomes and/or hemidesmosomes in oral epithelia. Whether retinoic acid disintegrated cell-cell and cell-matrix adhesions of oral epithelia was investigated with stratified gingival epithelial (GE1) cells. Ultrastructurally, retinoic acid-treatment (1 μM, 5-10 days) drastically reduced the number of desmosomes and completely destroyed hemidesmosomes. Keratinocyte phenotypes disappeared, accompanied by loss of tonofilaments. The transcripts of desmoglein 1 (ANOVA, P<0.01), desmocollin 1 (P<0.05), and keratin 13 (P<0.05) were significantly decreased, but expression of α6 and β4 subunits of integrin, components of hemidesmosomes, were not changed. These results indicated that retinoic acid also disintegrated desmosomes by depriving the cells of desmoglein 1 and desmocollin 1 in the oral mucosal epithelium. The decrease of keratin filaments, the intracellular cytoskeletal attachments to hemidesmosomes, was probably a cause of hemidesmosome loss.
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