| Project/Area Number |
12671785
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
ISOKAWA Keitaro Dentistry, Anatomy, Assistant Professor, 歯学部, 講師 (50168283)
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| Co-Investigator(Kenkyū-buntansha) |
SEJIMA Hitomi Dentistry, Anatomy, Research Assistant, 歯学部, 助手 (50287661)
SHIMIZU Osamu Dentistry, Maxillofacial Surgery, Research Assistant, 歯学部, 助手 (40260971)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2002: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | fibrillin / microfibrils / elastic fiber / oxytalan fiber / extracellular matrix / morphogenesis / limb bud / chick embryo / development / immunohistochemistry / immunoprecipitation |
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| Research Abstract |
The FBI monoclonal antibody we originally generated to a perichondrial matrix was shown to bind a molecule indistinguishable immunologically and immunohistochemically from the JB3 antigen ; i.e., chicken fibrilln-2. Fibrillin immunoreactivity in the form of fibrillar matrix was first detected in the primitive streak and around the Hensen's knot by stage 3. The following temporo-spatial distribution of fibrillin reactivity was distinct from the other well-described fibrillar matrices such as collagens and fibronectin during the embryonic development of the chick and quail. Each of the fibrillin-immunoreactive fibrils was basically a bundle of microfibrils which were ultrastructurally similar to the mammalian elastin-associated microfibrils with a diameter of 12-14 nm. Actually, elastin has accumulated within the bundle of microfibrils in the developing aorta and lung. However, fibrillin-immunoreactive microfibrils in many other embryonic tissues were devoid of amorphous elastin at least
… More
in the embryonic stages and appeared to form a unique, independent fibrillar network. Our experimental analysis using a technique of foot-binding combined with immunohistochemistry has revealed that dermal fibrils (bundles of microfibrils) originating from the basal lamina "tack" the ectoderm firmly to the underlying mesenchyme during the limb bud elongation and the armpit formation. Microfibrillar network in the developing gizzard was three-dimensionally organized as to form a cradle-like structure in which a large number of smooth muscle cells are encircled separately in a way logical to exert their contractile properties. In the heart, two types of fibrillin-positive matrices were demonstrated. Thicker extracellular fibrils through at the inner curvature have suggested their structural role in suspending the heart. A finer delicate fibrillar network enmeshing endothelially-derived mesenchymal cells in the cardiac cushions was positionally relevant to serve as a repository by which soluble factors such as TGF-beta 3 could be sequestered or released in the extracellular milieu because of the structural homology of fibrillin to a latent-type TGF-beta binding protein. Less
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