Phylogenetic Analysis of Hepatitis C Virus Infection in Dentistry
| Project/Area Number |
12671792
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Matsumoto Dental University |
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Principal Investigator |
HASEGAWA Hiromasa Matsumoto Dental University, School of Dentistry, Professor, 歯学部, 教授 (60164828)
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| Co-Investigator(Kenkyū-buntansha) |
UEMATSU Takashi Matsumoto Dental University, School of Dentistry, Associate Professor, 歯学部, 助教授 (40203476)
ESUMI Mariko Nihon University School of Medicine, Associate Professor, 医学部, 助教授 (30167291)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 2001: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2000: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Dentistry / Hemostatic gauze / HCV RNA / RT-PACR / Phylogenetic three |
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| Research Abstract |
Hemostatic gauze samples used for surgical treatments in 581 cases were randomly collected. HCV RNA was detected by reverse transcription of extracted RNA followed by nested PCR using primer pairs for the first and second PCR of HCV 5'-untranslated region. For phylogenetic analysis, cDNA was amplified by nested PCR using primer pairs of HCV HVR1.
Most chief complaints of 581 cases were chronic periodontitis and chronic apical periodontitis. HCV RNA was detected in eight cases (roughly 1.4 %) by the first or second PCR. Although seven of eight patients had the medical history of HCV infection, one patient gave us no information of HCV infection prior to the treatment. In seven cases, HCV antibodies were analyzed, and three patients were positive for HCV antibody.
Phylogenetic analysis demonstrated that isolated clusters were roughly divided into three groups. Interestingly, case 2 had a minor isolates related to a major cluster of case 1. This results cannot directly prove patient-to-patient transmission in our dental units, so further investigations of their clinical histories must be performed.
The incidence of HCV carriers of current series is relatively low, compared with that of Japanese general population (1.8-3.4 per 100,000 person). However, this study showed that dentistry is a possible route of the unrecognized transmission of HCV between patients and dental health worker as well as from patient to patient through lack of care regarding sterile procedures by staff, especially in the case treated without any information of HCV infection.
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Report
(4 results)
Research Products
(3 results)
