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Phylogenetic Analysis of Hepatitis C Virus Infection in Dentistry

Research Project

Project/Area Number 12671792
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionMatsumoto Dental University

Principal Investigator

HASEGAWA Hiromasa  Matsumoto Dental University, School of Dentistry, Professor, 歯学部, 教授 (60164828)

Co-Investigator(Kenkyū-buntansha) UEMATSU Takashi  Matsumoto Dental University, School of Dentistry, Associate Professor, 歯学部, 助教授 (40203476)
ESUMI Mariko  Nihon University School of Medicine, Associate Professor, 医学部, 助教授 (30167291)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 2001: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2000: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsDentistry / Hemostatic gauze / HCV RNA / RT-PACR / Phylogenetic three
Research Abstract

Hemostatic gauze samples used for surgical treatments in 581 cases were randomly collected. HCV RNA was detected by reverse transcription of extracted RNA followed by nested PCR using primer pairs for the first and second PCR of HCV 5'-untranslated region. For phylogenetic analysis, cDNA was amplified by nested PCR using primer pairs of HCV HVR1.
Most chief complaints of 581 cases were chronic periodontitis and chronic apical periodontitis. HCV RNA was detected in eight cases (roughly 1.4 %) by the first or second PCR. Although seven of eight patients had the medical history of HCV infection, one patient gave us no information of HCV infection prior to the treatment. In seven cases, HCV antibodies were analyzed, and three patients were positive for HCV antibody.
Phylogenetic analysis demonstrated that isolated clusters were roughly divided into three groups. Interestingly, case 2 had a minor isolates related to a major cluster of case 1. This results cannot directly prove patient-to-patient transmission in our dental units, so further investigations of their clinical histories must be performed.
The incidence of HCV carriers of current series is relatively low, compared with that of Japanese general population (1.8-3.4 per 100,000 person). However, this study showed that dentistry is a possible route of the unrecognized transmission of HCV between patients and dental health worker as well as from patient to patient through lack of care regarding sterile procedures by staff, especially in the case treated without any information of HCV infection.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Hiromasa Hasegawa: "Detection of Hepatitis C Virus Antibody and RNA in Hemostatic Gauze Used for Dentistry"Infect Control Hosp Epidemiol. 24. 137-139 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hiromasa Hasegawa: "Detection of Hepatitis C Virus Antibody and RNA in Hemostatic Gauze Used for Dentistry"Infect Control Hosp Epidemiol. 24, No2. 137-139 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hiromasa Hasegawa: "Detection of Hepatitis C Virus Antibody and RNA in Hemostatic Gauze Used for Dentistry"Infect Control Hosp Epidemiol. Vol24・No2. 137-139 (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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