The ultrastructure of osteoclast ruffled border and resorption lacuna

Research Project

Project/Area Number	12671793
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Morphological basic dentistry
Research Institution	Asahi University
Principal Investigator	YOSHIDA Hisaho Asahi University, School of Dentistry, assistant, 歯学部, 助手 (80102119)
Co-Investigator(Kenkyū-buntansha)	AKISAKA Toshitaka Asahi University, School of Dentistry, professor, 歯学部, 教授 (70116523)
Project Period (FY)	2000 – 2001
Project Status	Completed (Fiscal Year 2001)
Budget Amount *help	¥2,500,000 (Direct Cost: ¥2,500,000) Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000) Fiscal Year 2000: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywords	Osteoclast / ruffled border / cytoskeleton / ultrastructure / deep-etching / quick freezing / 急速冷凍凍結置換 / 凍結乾燥 / 細胞裏打ち構造 / 急速凍結凍結置換 / 細胞膜裏打ち構造
Research Abstract	We studied the ruffled border of osteoclasts at ultrastructural levels by quick-frozen, deep-etched, and rotary shadowed replicas. In freeze substituted section, the ruffled border was reported finger-shaped or foliate by Akisaka et al., 1997. However, three-dimensional structure of the ruffled border are not known. The deep-etched electron microscopy revealed the same image as freeze-substituted sections, but also provided three-dimensional information. Complicated infoldings of ruffled border was distinguished in the deep-etched replica. When the fracture plane passed through the ruffled border, the reticular network as cytocskeletal of the fingers and foliate became visible. The reticular network fills the innercellular as a cytoplasmic core. These filaments appeared to do contact with the inner surface of the ruffled border membrane. The direct relation between the intramembranos particles of the ruffled border and the reticular network, contact may be integral to association of polarization and activation with the plasma membranes for bone resorption. On the other hand, baso-lateral membran does not appeared infoldings of plasma membrane.