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Involvement of PPAR γ in senescence-induced osteoporosis

Research Project

Project/Area Number 12671799
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionTokyo Medical and Dental University

Principal Investigator

MORITA Ikuo  Tokyo Medical and Dental University, Graduate School, Associate Professor, 大学院・医歯学総合研究科, 助教授 (60100129)

Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2000: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsOsteoblasts / Adipocytes / Osteoclasts / PPAR / Cbfa-1 / CBP / α / Human mesenchymal cells / 骨芽細胞分化 / 脂肪細胞分化 / BMP-2 / PPARγ / 老人性骨粗鬆症 / 間葉系幹細胞
Research Abstract

Senescence-induced osteoporosis is thought to be a low turnover type and to be due to the shift from osteoblastogenesis to adipogenesis. In this study, we investigated the effects of some drugs affected bone metabolism on adipose differentiation and the effects of adipogenesis on osteoclastogenesis and osteoblastogenesis. We used mouse preosteoblast cell lines established in our laboratory and human mesenchymal stem cells isolated from bone marrow. The PPARγ ligands such as 15-deoxy.-Δ 12,14-PGJ2 and trogritazone stimulated adipogenesis, but IL-11 and vitaminK2 suppressed the adipogenesis. The adipocyte differentiation caused to decrease the osteoclast formation-supporting activity, but did not affect osteoblastogenesis. The similar results were obtained from in vivo study. When human mesenchymal cells with BMP-2 and vitamin K2 inoculated to Matrigel and transplanted subcutaneously to nude mice, the cells was differentiated to osteoblasts and adipocytes by BMP-2. The effect of vitamin K2 on osteoblastogenesis failed to be observed in spite of the suppression of adipogenesis.
These data indicate that the suppression of adipogenesis is not benefit for the protect of senescence-induced osteoporosis.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Sakaguchi K: "Relationship between the ability to support differentiation of osteoclast-like cellsand adipogenesis in murine stromal cells derived from bone marrow"Prostaglandins Leukotrienes & Essential Fatty Acids. 62・5. 319-327 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Gao Y: "Expression of adhesion molecules LEA-1 and ICAM-lon osteoclast precursors during osteoclast differentiation and involvement of estrogen deficiency"CLIMACTERIC. 3. 278-287 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Palacious VG: "Expression of adipogenesis markers in a murine stromal cell line treated with 15-deoxy Δ 12,14-prostaglandin 12,intereleukin-11,9-cis retinoic acid and vitamin K2"Prostaglanidn Leukotriene & Essential Fatty Acids. 65.4. 215-221 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakaguchi K: "Relationship between the ability to support differentiation of osteoclastlike cells and adipogenesis in murine stromal cells derived from bone marrow"Prostaglandin Leukotriene & Essential Fatty Acids. 62.5. 319-327 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Gao Y: "Expression of adhesion molecules LFA-1 and ICAM- 1 on osteoclast precursors during osteoclast differentiation and involvement of estrogen deficiency"Climacteric. 3. 278-287 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Palacious VG: "Expression of adipogenesis markers in a murine stromal cell line treated with 15-deoxy Δ12,14-prostaglandin J2, intereleukin-11,9-cis retinoic acid and vitamin K2"Prostaglandin Leukotriene & Essential Fatty Acids. 65.4. 215-221 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Palacious VG: "Expression of adipogenesis markers in a murine stromal cell line treated with 15-deoxy- Δ 12, 14-prostaglanidn J2, interleukin-11, 9-cis retinoic acid and vitamin K2"Prostaglanidn Leukotriene & Essential Fatty Acids. 65・4. 215-224 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sakaguchi K: "Relationship between the ability to support differentiation of osteoclast-like cellsand adipogenesis in murine stromal cells derived from bone marrow"Prostaglandins Leukotrienes & Essential Fatty Acids. 62・5. 319-327 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Gao Y : "Expression of adhesion molecules LFA-1 and ICAM-1on osteoclast precursors during osteoclast differentiation and involvement of estrogen deficiency"CLIMACTERIC. 3. 278-287 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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