| Project/Area Number |
12671806
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
NAKANISHI Tohru Okayama Univ., Grad. Sch. of Med. and Dent., Associate Professsor, 大学院・医歯学総合研究科, 助教授 (30243463)
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| Co-Investigator(Kenkyū-buntansha) |
OHYAMA Kazumi Okayama Univ., Dental School, Instructor, 歯学部, 教務員 (00253021)
TAKIGAWA Masaharu Okayama Univ., Grad. Sch. of Med. and Dent., Professsor, 大学院・医歯学総合研究科, 教授 (20112063)
服部 高子 岡山大学, 歯学部, 助手 (00228488)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | microarray / ATDC5 / chemokine / osteosrthritis / rheumatoid arthritis / caspase / MAP kinase / transgenic mice / 軟骨細胞 / CTGF / アポトーシス / caspase-9 / DNA発現アレイ / HSP47 |
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| Research Abstract |
1) Gene expression in a pre-chondrocytic cell line ATDC5 was analyzed by using DNA microarrays. Several genes showed significant difference of expression level between undifferentiated and differentiated stage of ATDC5 cells.
2) Gene expression in RA (rheumatoid arthritis)- or OA (osteoarthritis)-derived synoviocytes was analyzed by using DNA microarrays. The expression of STAT, PKC, caspase and IGFBP was upregulated in OA, and the expression of CDC25, RHO and FGF7 was up-regulated in RA. The expression of c-fos and c-jun was also up-regulated in RA. Immunostaining showed that apoptosisrelated caspase-9 was highly expressed in OA-derived synoviocytes and cartilage tissues.
3) Gene expression in a chondrosarcoma-derived chondrocytic cell line, HCS-2/8 was analyzed by using DNA microarrays.The expression of several MAP kinases was up-regulated by the addition of CTGF. We found that CTGF stimulated the proliferation of HCS-2/8 cells through Erk, and the differentiation of HCS-2/8 cells through p38 MAPK.
4) CTGF-overexpressed transgenic mice were prepared by injection of expression vectors in which CTGF was expressed under the control of type XI collagen promoter into fertilized eggs. The expression of exogenous CTGF was observed in the cartilage tissues of transgenic mice, but its endogenous expression was decreased in transgenic mice. It was also showed that transgenic mice had the phenotype of dwarfism with decreased bone density.
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