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Analysis of gene expression in chondrocytes using DNA microarrays (DNA chips)

Research Project

Project/Area Number 12671806
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

NAKANISHI Tohru  Okayama Univ., Grad. Sch. of Med. and Dent., Associate Professsor, 大学院・医歯学総合研究科, 助教授 (30243463)

Co-Investigator(Kenkyū-buntansha) OHYAMA Kazumi  Okayama Univ., Dental School, Instructor, 歯学部, 教務員 (00253021)
TAKIGAWA Masaharu  Okayama Univ., Grad. Sch. of Med. and Dent., Professsor, 大学院・医歯学総合研究科, 教授 (20112063)
服部 高子  岡山大学, 歯学部, 助手 (00228488)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordsmicroarray / ATDC5 / chemokine / osteosrthritis / rheumatoid arthritis / caspase / MAP kinase / transgenic mice / 軟骨細胞 / CTGF / アポトーシス / caspase-9 / DNA発現アレイ / HSP47
Research Abstract

1) Gene expression in a pre-chondrocytic cell line ATDC5 was analyzed by using DNA microarrays. Several genes showed significant difference of expression level between undifferentiated and differentiated stage of ATDC5 cells.
2) Gene expression in RA (rheumatoid arthritis)- or OA (osteoarthritis)-derived synoviocytes was analyzed by using DNA microarrays. The expression of STAT, PKC, caspase and IGFBP was upregulated in OA, and the expression of CDC25, RHO and FGF7 was up-regulated in RA. The expression of c-fos and c-jun was also up-regulated in RA. Immunostaining showed that apoptosisrelated caspase-9 was highly expressed in OA-derived synoviocytes and cartilage tissues.
3) Gene expression in a chondrosarcoma-derived chondrocytic cell line, HCS-2/8 was analyzed by using DNA microarrays.The expression of several MAP kinases was up-regulated by the addition of CTGF. We found that CTGF stimulated the proliferation of HCS-2/8 cells through Erk, and the differentiation of HCS-2/8 cells through p38 MAPK.
4) CTGF-overexpressed transgenic mice were prepared by injection of expression vectors in which CTGF was expressed under the control of type XI collagen promoter into fertilized eggs. The expression of exogenous CTGF was observed in the cartilage tissues of transgenic mice, but its endogenous expression was decreased in transgenic mice. It was also showed that transgenic mice had the phenotype of dwarfism with decreased bone density.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] Nakanishi, T.: "Effects of CTGF/Hcs24, a product of a hypertrophic chondrocyte-specific gene, on the proliferation and differentiation of chondrocytes in culture"Endocrinology. 141. 264-273 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakanishi, T.: "Japan may seek embryo cells from overseas"Nature. 405. 882 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakanishi, T.: "Overexpression of connective tissue growth factor/hypertrophic chondrocyte specific gene product 24 (CTGF/Hcs24) decreases bone density in adult mice and induces dwarfism"Biochem.Biophys.Res.Commun.. 281. 678-681 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakanishi, T.: "Recent advances in DNA microarrays"Acta Med. Okayama. 55. 319-328 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshimichi, G. et al.: "CTGF/Hcs24 induces chondrocyte differentiation through p38 mitogen-activated protein kinase (p38MAPK), and proliferation through p44/42 MAPK/extracellular-signal regulated kinase (ERK)"Eur.J.Biochem.. 268. 6058-6065 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 中西 徹: "マイクロアレイの解析によるリウマチ病因遺伝子の解析"感覚器障害および免疫・アレルギー等研究事業研究抄録集. 50 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 中西 徹 他: "軟骨内骨化の分子制御 新・分子骨代謝学と骨粗鬆症(松本俊夫編)"メディカルレビュー社. 14 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakanishi, T.: "Effects of CTGF/Hcs24, a product of a hypertrophic chondrocyte-specific gene, on the proliferation and differentiation of chondrocytes in culture."Endocrinology. 141. 264-273 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakanishi, T.: "Japan may seek embryo cells from overseas."Nature. 405. 882 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakanishi, T.: "Overexpression of connective tissue growth factor/hypertrophic chondrocyte-specific gene product 24 (CTGF/Hcs24) decrease bone density in adult mice and induces dwarfism."Biochem. Biophys. Res. Commun.. 281. 678-681 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakanishi, T.: "Recent advances in DNA microarrays."Acta Med. Okayama. 55. 319-328 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nishida, T.: "Effects of CTGF/Hcs24, a hypertrophic chondrocytespecific gene product, on the proliferation and differentiation of osteoblastic cells in vitro."J. Cell. Physiol.. 184. 197-206 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shakunaga, T.: "Expression of connective tissue growth factor in cartilaginous tumors."Cancer. 89. 1466-1473 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamashiro, T.: "Mechanical stimulation induces CTGF expression in rat osteocytes."J. Dent. Res. 80. 461-465 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kubota, S.: "Novel mode of processing and secretion of connective tissue growth factor/ecogenin (CTGF/Hcs24) in chondrocytic HCS-2/8 cells."Bone. 29. 155-161 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Eguchi, T.: "Regulatory mechanism of human connective tissue growth factor (CTGF/Hcs24) gene expression in a human chondrocytic cell line HCS-2/8."J. Biochem.. 130. 79-87 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shimo, T.: "Involvement of CTGF, a hypertrophic chondrocyte-specifi gene product, in tumor angiogenesis."Oncology. 61. 315-322 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shimo, T.: "Connective tissue growth factor as a major angiogenic agent that is induced by hypoxia in a human breast cancer cell line."Cancer Lett.. 174. 57-64 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Pan, L.H.: "Type II alveolar epithelial cells and interstitial fibroblasts express connective tissue growth factor in IPF."Eur. Respir. J.. 17. 1220-1227 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshimichi, G.: "CTGF/Hcs24 induces chondrocyte differentiatior through p38 mitogen-activated protein kinase (p38MAPK), and proliferation through p44/42 MAPK/extracellular-signal regulated kinas (ERK)."Eur. J. Biochem.. 268. 6058-6065 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Oka, T.: "Reduction of hematopoietic cell-specific tyroshine phosphatase SHP-1 gene expression in natural killer cell lymphoma an various types of lymphomas/leukemias : Combination analysis with cDNA expression array and tissue microarray."American J. Pathol.. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakanishi, T.et al.: "Overexpression of connective tissue growth factor/hypertrophic chondrocyte-specific gene product 24 (CTGF/Hcs24) decreases bone density in adult mice and induces dwarfism"Biochem. Biophys. Res. Commun.. 281. 678-681 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nakanishi, T.et al.: "Recent advances in DNA microarrays"Acta Med.Okayama. 55. 319-328 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yoshimichi, G.et al.: "CTGF/Hcs24 induces chondtrocyte differentiation through a p38 mitogen-activatcd protein kinase (p38MAPK), and proliferation through a p44/42 MAPK/extracellular-signal regulated kinase (ERK)"Eur.J.Biochem.. 268. 6058-6065 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shimo, T.et al.: "Involvement of CTGF, a hypertrophic chondrocyte-specific gene product, in tumor angiogenesis"Oncology. 61. 315-322 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shimo, T et al.: "Connective tissue growth factor asa a major angiogenic agent that is induced by hypoxia in a human breast cancer cell line"Cancer Lett.. 174. 57-64 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 中西 徹: "マイクロアレイの解析によるリウマチ病因遺伝子の解析"感覚器障害および免疫・アレルギー等研究事業研究抄録集. 50 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 中西 徹 他: "軟骨内骨化の分子制御 新・分子骨代謝学と骨粗鬆症(松本俊夫編)"メディカルレビュー社. 14 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nakanishi,T.: "Effects of CTGF/Hcs24, a product of a hypertrophic chondrocyte-specific gene, on the proliferation and differentiation of chondrocytes in culture."Endocrinology. 141. 264-273 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nakanishi,T.: "Overexpression of connective tissue growth factor/hypertrophic chondrocytespecific gen product 24 (CTGF/Hcs24) decreases bone density in adult mice and induces dwarfism."Biochem.Biophys.Res.Commun.. (印刷中).

    • Related Report
      2000 Annual Research Report
  • [Publications] Hattori,T.: "Rheumatoid arthritis related antigen 47 kDa (RA-A47) is a product of colligin-2 and acts as a human HSP47."J.Bone Miner.Metab.. 18. 328-334 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hattori,T.: "Change in cellular localization of a rheumatoid arthritis-related antigen (RA-A47) with downregulation upon stimulation by inflammatory cytokines in chondrocytes."J.Cell.Physiol.. 186. 268-281 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nakanishi,T.: "Japan may seek embryo cells from overseas."Nature. 405. 882 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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