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Regulation of bicarbonate secretion as fluid excretion in the salivary ducts

Research Project

Project/Area Number 12671808
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

SHIBA Yoshiki  Faculty of Dentistry, Department of Oral Physiology, Hiroshima University, Professor, 歯学部, 教授 (90110452)

Co-Investigator(Kenkyū-buntansha) SUGITA Makoto  Faculty of Dentistry, Department of Oral Physiology, Hiroshima University, Research associate, 歯学部, 助手 (50235884)
HIRONO Chikara  Faculty of Dentistry, Department of Oral Physiology, Hiroshima University, Associate professor, 歯学部, 助教授 (10199135)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2001: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsDuct secretion / Salivary secretion / ion channel / CFTR / bicarbonate / bicarbonate secretion
Research Abstract

In the present study, we investigated the exclusion of fluorescent dye from the duct lumen by carbachol (CCh) in isolated parotid interlobular duct segments to clarify the ability of the ducts for the fluid secretion with bicarbonate secretion. CCh decreased the fluorescent intensity in the duct lumen during the superfusion of the fluorescent dye, and CCh flushed out small viscous substances stained with the fluorescent dye from isolated duct lumen. Methazolamide and removal of external Ca^<2+> suppressed CCh-induced clearance of the duct fluorescence. A major portion was inhibited by removal of bicarbonate in the superfusion solution and DPC, but not low concentration of external CT, bumetanide or DIDS. Patch-clump investigation indicated the presence of Ca^<2+> -activated anion channels, and gramieidin-perforated patched analysis indicated that bicarbonate was a major portion of outward currents in the isolated duct cells. These results suggest that carbachol evoles fluid secretion possibly through Ca^<2+> -activated, DPC-sensitive anion channels with HCO_3^- secretion in the rat parotid intralobular ducts.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Hirono, C., Nakamoto, T., Sugita, M., Iwasa, Y., Akagawa, A., Shiba, Y.: "Gramicidin-perforated patched analysis on HCO3-secretion through a forskolin-activated anion channel in rat parotid intralobular duct cells"J.membrane Biol.. 180. 11-19 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirono C, Nakamoto T, Sugita M, Iwasa Y, Akagawa A, and Shiba Y: "Gramicidin-perforated patch analysis on HCO3-secretion through a forskolin-activated anion channel in rat parotid intralobular duct cells"J. Membrane Biol. 180. 11-19 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirnono, C., Nakamoto, T., Sugita, M., Iwasa, Y., Akagawa, A., Shiba, Y.: "Gramicidin-perforated patched analysis on HCO3-secretion through a forskolin-activated anion channel in rat parotid intralobular duct cells."J. membrane Biol.. 180. 11-19 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hirono,C.,Nakamoto,T.,Sugita,M.,Iwasa,Y.,Akagawa,A.and Shiba,Y.: "Gramicidin-perforated patch analysis on HCO3- secretion through a forskolin-activated anion channel in rat parotid intralobular duct cells."J.Membrane Biol.. 180(In press). (2001)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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