| Project/Area Number |
12671810
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Functional basic dentistry
|
|---|
| Research Institution | NAGASAKI UNIVERSITY GRADUATE SCHOOL OF BIOMEDICAL SCIENCES |
|---|
Principal Investigator |
MIYAMOTO Takenori INTEGRATIVE SENSORY PHYSIOLOGY, RESEARCH ASSOCIATE, 大学院・医歯薬学総合研究科, 助手 (10167679)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MIYAZAKI Toshihiro ORALCYTOLOGY AND CELL BIOLOGY, RESEARCH ASSOCIATE, 大学院・医歯薬学総合研究科, 助手 (10174161)
|
|---|
| Project Period (FY) |
2000 – 2002
|
| Project Status |
Completed (Fiscal Year 2002)
|
| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥1,300,000 (Direct Cost: ¥1,300,000)
|
|---|
| Keywords | Taste cell / Taste stmuli / transmitter / carbonfiber electrode / patch clamp / bioassay / paracrine / vallate papilla / von Ebner's gland complex / アミノ酸 / アセチルコリン / ペプチド / 有廓乳頭味細胞 / エブネル腺 / 電気化学的検出法 / セロトニン / グルタミン酸 / マウス / 味神経 / 塩味 / うま味 |
|---|
| Research Abstract |
Immunohistochemical studies have demonstrated that taste receptor cells containing serotonin exist in taste buds. In the previous studies, we detected serotonin secreted from the serosal side of the lingual epithelium in response to the repeated application of NaCl or citric acid to the mucosal side of the lingual epithelium using full-automatical perfusion/sampling device for epithelial tissue and HPLC. Therefore, we attempted to detect serotonin secreted from a single taste bud in response to the application of taste stimuli to the taste pore using localized stimulation procedure and carbon fiber electrodes. However, we were unable to detect any monoamines. We attempted to immunohistochemically detect localization of several subtypes of glutamatergic receptor molecules in the taste buds of the fungiform papilla. One of the metabotropic glutamatergic receptors, mGluR4 was detected in the taste bud cells, but not in the gustatory nerve terminal of the afferent synapse. Taken together w
… More
ith electrophysiological data, mGluR4 in the taste bud cells possibly play an inhibitory role for transduction mechanism of umami. These results suggest mat substances other than glutamate and monoamines mediate the transmission from taste receptor cells to gustatory nerves. Therefore, we performed a bioassay using vallate papilla (VP)/von Ebner's gland (VEG) complex, where several receptor molecules are expressed on the acinar cells. The results indicated that localized bitter stimuli to the cleft of isolated vallate papilla suppressed voltage-gated K^+ currents of acinar cells, whereas sour stimuli induced inward current responses in acinar cells with slow time course, suggesting that these actions of taste stimuli are due to paracrine effect of transmitters released from taste bud cells. These findings also suggest that transmitter released by bitter stimulus is different from that by sour stimulus, and receptor molecules for these multiple transmitters were expressed on acinar cells of VEG. Less
|
