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The Role of Peroxisomes in Bone Formation

Research Project

Project/Area Number 12671812
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionKyushu Dental College

Principal Investigator

FUJIWARA Satoko  Dept. of Dentistry, Kyushu Dental College, Associate Professor, 歯学部, 助教授 (20047806)

Co-Investigator(Kenkyū-buntansha) NOGUCHI Tomoo  Dept. of Dentistry, Kyushu Dental College, Professor, 歯学部, 教授 (30073688)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsPeroxisomes / Catalase / MC3T3-E1 cell / Uricase / Epiphyseal cartilage / D-aspartate oxidase / D-amino acid oxidase / Sucrose density gradient centrifugation / ペルソキシソーム / 軟骨細胞
Research Abstract

1. The morphology, distribution and biological function of peroxisomes in the fetal bovine articular cartilage of 5-month-old.
Sucrose density gradient centrifugation of the homogenate from chondrocytes of the fetal bovine cartilage was performed.
(1) The fetal bovine cartilage contained normal size peroxizomes with density of about 1.25, although it had been reported that the tissues out of liver and kidney contained microperoxisomes with low density.
(2) Alanine : glyoxylate aminotransferase(AGT) which is only animotransferase in animal peroxisomes and is wide distributed in mammalian liver. AGT activity was not detected in peroxisomes of the fetal bovine cartilage, suggested that the function of peroxisomes in cartilage was different from liver.
(3) D-aspartate oxidase(D-Asp O) was localized in peroxisomes in the fetal bovine cartilage, suggested that D-saparate was present in this tissue and D-Asp O was related to the developmental processes.
2. The morphology, distribution and biological function of peroxisomes in MC3T3-E1 cell (osteoblast- like cell derived from mouse).
Catalase activity was detected in the homogenate of MC3T3-E1 cells, suggested that peroxisomes were present in MC3T3-E1 cell. Other peroxisomal marker enzyme activities were not detected in the homogenate. Therefore, the proliferation of peroxisomes by clofibrate (peroxisomal proliferator) treatment and some peroxisomal enzyme activities must be investigated further.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Masuda, W.: "A new type of allantoinase in amphybian liver"Biosci. Biotechnol. Biochem.. 65. 2558-2560 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Maki, K.: "A new type of Matrix vesicles is found in fetal bovine tracheal cartilage"Conective Tissue Research. 41(2). 109-115 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hayashi, S.: "Evolution of urate-degrading enzymes in animal peroxisomes"Cell Biochem. Biophys.. 32. 123-129 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masuda, W.: "ADP-ribosyl cyclase in rat salivary glands"Biochim. Biophys. Res. Commum.. 270. 469-472 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakuraba, H.: "Metabolism of glyoxylate, the end product of purine degradation in liver peroxisomes of freshwater fish"Biochim. Biophys. Res. Commum.. 228. 603-606 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fujiwara, S.: "Degradation of purines: only ureidoglycollate lyase out of degradingenzymes is present in mammals"Biochem. J.. 312. 315-318 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Noguchi, T.: "Comparative Biochemistry of Animal Peroxisomes"Research Signpost. 13 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masuda W., Fujiwara S. and Noguchi T.: "A new type of allantoinase in amphybian liver"Biosci. Biotechnol. Biochem.. 65 (11). 2558-2560 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Maki K., Hayashi S., Nishioka T., Kimura M. and Noguchi T.: "A new type of matrix vesicles is found in fetal bovine tracheal cartilage"Conective Tissue Research. 41 (2). 109-115 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masuda W. and Noguchi T.: "ADP-ribosyl cyclase in rat salivary glands"Biochim. Blophys. Res. Commum.. 270. 469-472 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hayashi S., Fujiwara S. and Noguchi T.: "Evolution of urate-degrading enzymes in animal peroxisomes"Cell Biochem. Biophys. 32. 123-129 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakuraba H., Fujiwara S. and Noguch T.: "Metabolism of glyoxylate, the end product of purine degradation in liver peroxisomes of freshwater fish"Biochim. Biophys. Res. Commum.. 229. 603-606 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fujiwara S. and Noguchi T.: "Degradation of purines : only ureidoglycollate lyase out of degrading enzymes is present in mammals"Biochem. J.. 312. 315-318 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Noguchi T., Fujiwara S., Hayashi S., and Sakuraba H.: "Amino acid and glyoxylate metabolism"Research Signpost, 1-7, 1997 Comparative Biochemistry of Animal Peroxisomes.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Noguchi T., Fujiwara S., Hayashi S. and Sakuraba H.: "Purine metabolism"Research Signpost, 8-13, 1997 Comparative Biochemistry of Animal Peroxisomes.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Wataru Masuda: "A new Type of allantoinase in Amphibian Liver"Biosci. Biotechnol. Biochem.. 65(11). 2001-2558 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sueko Hayashi: "Evolution of Urate-Degrading Enzymes in Animal Peroxisomes"Cell Biochemistry and Biophysics. 32. 125-129 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Maki K.: "A New Type of Matrix Vesicles is Found in Fetal bovine"Connect Tissue Res.. 41(2). 109-115 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Masuda. W.: "ADP-Ribosyl cyclase in Rat Salivaryl Glands"Biochem. Biophys. Res. Commun.. 270. 469-472 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Maki,K.: "A New Type of Matrix Vesicles is Found in Fetal Bovine Tracheal cartilage"Connect Tissue Res.. 41・2. 109-115 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hayashi,S.: "Evolution of urate-degrading enzymes in animal peroxisomes"Cell Biochemistry and Biophysics. 32. 123-129 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sakuraba,H.: "Metabolism of glyoxylate, the end product of purine degradation,in liver peroxisomes of fresh water fish"Biochem.Biophys.Res.Commun.. 229. 603-606 (1996)

    • Related Report
      2000 Annual Research Report
  • [Publications] Fujiwara,S.: "Degradation of purines : Only ureidoglycollate lyase out of four allantoin-degrading enzymes is present in mammals"Biochem.J.. 312. 315-318 (1995)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sakuraba,H.: "Alcohol : NAD oxdoreductase is present in rat liver peroxisomes"J.Biol.Chem.. 270. 37-40 (1995)

    • Related Report
      2000 Annual Research Report
  • [Publications] Noguchi,T.: "Comparative Biochemistry of Animal Peroxisomes,(ed.T.Noguchi)"Research Signpost,India. 14 (1997)

    • Related Report
      2000 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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