Caspase cascades which activated by ionizing irradiation
| Project/Area Number |
12671823
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
病態科学系歯学(含放射線系歯学)
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| Research Institution | Hokkaido University |
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Principal Investigator |
YASUDA Motoaki Hokkaido Univ., School of Dent., Asso. Prof., 大学院・歯学研究科, 助教授 (90239765)
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| Co-Investigator(Kenkyū-buntansha) |
SHINDOH Masanobu Hokkaido Univ., School of Dent., Asso. Prof., 大学院・歯学研究科, 助教授 (20162802)
HIGASHINO Fumihiro Hokkaido Univ., School of Dent., Lec., 大学院・歯学研究科, 助手 (50301891)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2000: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Apoptosis / Radiation / DNA array / 放射線照射 / Caspase |
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| Research Abstract |
1. Radiation resistance of human normal and tumor cell lines.
An enzymatic activities of caspase-8 and caspase-9 of human normal (MRC5) and tumor (HL60, MGF7) were analyzed after 10Gy gamma-irradiation. HL60 expressed a significant amount of activated caspases, whereas MRC5 showed only a few amount of active caspase-9 (no active caspase-8 was detected). We concluded that radiation resistance and an amount of active caspase were well corresponding.
2. A conditioned medium from irradiated MRC5 contained elevated amount of chemoattractants.
We found that irradiated MRC5 derived conditioned medium contained increased amount of chemoattractants. Northern blotting and competitive activity of anti-HGF antibodies indicate that one of these chemoattractants is HGF.
3. Ionizing radiation induced gene expression (DNA array analysis)
We performed DNA analysis for irradiated MRC5. There were no significant changes in bcl-2 related genes. We found the up-regulation of caspase-2,-6 and 10. We also found the up-regulation of apoptosis suppressing genes. We concluded that these up-regulation of anti-apoptotic proteins may explain the radiation resistance of normal connective tissue.
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Report
(3 results)
Research Products
(4 results)
