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A study Of ora| streptococcal histone-like protein and virulence mechanism

Research Project

Project/Area Number 12671827
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 病態科学系歯学(含放射線系歯学)
Research InstitutionThe University of Tokushima

Principal Investigator

HIROTA Katsuhiko  The University of Tokushima, Dentstry, Reaearch Associate, 歯学部, 助手 (60199130)

Co-Investigator(Kenkyū-buntansha) NEMOTO Ken  The University of Tokushima, Dentstry, Reaearch Associate, 歯学部, 助手 (10218274)
ONO Tsuneko  The University of Tokushima, Medicin, Professor, 医学部, 教授 (40035514)
MAYAKE Yoichiro  The University of Tokushima, Dentstry, Professor, 歯学部, 教授 (80136093)
MURAKAMI Keiji  The University of Tokushima, Dentstry, Reaearch Associate, 歯学部, 助手 (10335804)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsS. intermedius / histone-like protein / heparin / CD15s / S. intermedius / レンサ球菌 / ヒストン様蛋白 / 三次元構造 / 分子モデル / 心疾患 / 腎疾患
Research Abstract

Streptococcus intermedius is associated with endogenous infections leading to abscesses in the oral cavity and deep-seated sites, such as the brain and liver. Histone-like protein (HLP), which binds to DNA and RNA molecules and to lipoteichoic acid and epithelial cells, is well conserved among bacterial species and induces macrophages to produce interleukin (IL)-1 and tumor necrosis factor (TNF)-α. In this study, we cloned the gene encoding S. intermedius HLP (HLPSi) from chromosomal DNA by use of the polymerase chain reaction (PCR) using the primers based on S. pyogenes hip gene and ligated to the glutathione S-transferase (GST) fusion vector. The encoded HLPSi had 91 amino acids; and its molecular weight and isoelectric point calculated from the sequence were 9,603 and 10.21 , respectively. The sequence of HLPSi had a high identity with those sequences of the HLP of S. pyogenes A374 and Staphylococcus aureus Mu50 and showed high homology with a 21 -kDa molecule of Mycobavterium leprae (ML-LBP21) at the amino acid level. The immunoelectron micrograph of S. intermedius by anti-HLPSi antibody and ELISA demonstrated that a part of HLPSi was localized on the cell surface and that recombinant HLPSi (rHLPSi) bound to heparin. Our results suggest that HLP produced from bacterial cells may play various roles in pathogenesis by helping the bacteria to bind to their target tissue within the infected host and may contribute to tissue injury at the infection site.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] 弘田克彦: "レンサ球菌のヒストン様蛋白質の構造解析"Bacterial Adherence研究. 14. 6-8 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoko Terada: "Identification of Helicobacter pylori in denture plaque of hospitalized aged patients"Dentistry in Japan. 37. 56-58 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Goto: "Rapid identification of Streptococcus interrmedius by PCR with the ily gene as a species marker gene"J. Med Microbiol.. 51. 178-186 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katsuhiko Hirota: "Three-dimensional structural analysis of histone-like protein from streptococci"Bacterial Adherence Researcl. 14. 6-8 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoko Terada: "ldentification of Helicobacter pylori in denture plaque of hospitalized aged patients"Dentistry in Japan. 37. 56-58 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Goto: "Rapid identification of Streptococcus intermedius byPCR with the ily gene as a species marker gene"J. Med. Microbiol. 51. 178-186 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoko Terada: "Identification of Helicobacter pylori in denture plaque of hospitalized aged patients"Dentistry in Japan. 37. 56-58 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Goto: "Rapid identivication of Streptococcus intermedius by PCR with the ily gene as a species marker gene"J. Med. Microbiol.. 51. 178-178 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 弘田克彦: "レンサ球菌のヒストン様蛋白質の構造解析"Bacterial Adherence研究. 14(印刷中). (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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