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Diagnosis of oral cancer by ^<11>C-Choline PET

Research Project

Project/Area Number 12671837
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 病態科学系歯学(含放射線系歯学)
Research InstitutionIwate Medical University

Principal Investigator

SHOZUSHIMA Masanori  Iwate medical university, School of dentistry, Associate, 歯学部, 助教授 (00118259)

Co-Investigator(Kenkyū-buntansha) SATO Hitoshi  Iwate medical university, School of dentistry, Research assistant, 歯学部, 助手 (40316401)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywords^<11>C-Choline / PET / oral cancer / cell cycle / 11C-コリン / 口腔癌 / グルコース / コリン / 癌細胞
Research Abstract

Positron emission tomography using fluorine-18 fluoro-deoxyglucose (FDG PET) is useful of the detection of malignant tumor recurrences and for the evaluation of a therapeutic response. Recently, ^<11>C- Choline was introduced as another tumor tracer and successfully visualized malignant tumor. In this research, we evaluated possibility to clinical application of ^<11>C-Choline PET. The relationship between ^<11>C-choline uptake and the cell cycle phase in HeLa S3 cells, as well as how they compare to the conventional tracer FDG and ^<11>C-Methionine (Met)
At first we constructed a computer -controlled apparatus of ^<11>C-Choline synthesis for routine clinical application of PET. 2-dimethylaminoethanol was loaded onto a Sep-Pak Accell CM cartridge, subsequent to the trapping of ^<11>C-methyl iodide. As a result, the average amount of ^<11>C Choline at end of synthesis was 140 mCi, the radiochemical purity was more than 99%. The simplicity of this method and the use of disposable materials for purification way be advantageous for routine clinical use. FDG uptake in HeLa S3 cells was significantly higher in the early S phase and G2/M phase compared to the G1 phase. In addition, Met and ^<11>C-Choline uptakes were higher in the G2/M phase. It has been concluded cell cycle dependency is reflected in the uptake of FDG, Met and ^<11>C-Choline seen during PET imaging of tumor tissue. These results reveal tumor proliferative activity, and can assist in evaluating a therapeutic response.
Furthermore, ^<11>C-Choline was administered to a rabbit neoplasm model, and it was observed in PET, and neoplasm locus was identified precisely, and what imaging of neoplasm could apply was shown in ^<11>C-Choline-PET.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] 小豆島 正典: "細胞分裂過程におけるFDGと11C-methionineの集積特性"NMCC共同利用研究成果報文集. 9巻(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 守口 斉: "HeLa S3細胞に対するFDGと67Ga citrate集積の細胞周期依存性"岩手医科大学歯学誌. 27(3). 270-278 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakamura R.: "Failure in presumption of residual disease by quantification of FDG uptake in esophageal squamous cell carcinoma immediately after radiotherapy"Radiation Medicine. 20(4). 181-186 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 泉澤 充: "ウサギVX2誘発舌癌転移リンパ節における18FDG集積"NMCC共同利用研究成果報文集. 8巻. 235-239 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 鎌田潤也: "心磁図による生存心筋の評価〜糖負荷18FDG-PET画像との対比〜"NMCC共同利用研究成果報文集. 8巻. 31-33 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 寺崎一典: "オンカラム法を用いた[11C]コリン自動合成装置の作成"NMCC共同利用研究成果報文集. 8巻. 265-272 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 小豆島 正典: "培養細胞へのFDG集積とCholine集積に対する細胞周期依存性"NMCC共同利用研究成果報文集. 8巻. 240-246 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 小豆島 正典: "HeLa細胞のDNA合成過程における18FDG取り込み変化"NMCC共同利用研究成果報文集. 7巻. 273-278 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 小豆島正典: "第3版 歯科放射線学"医歯薬出版(株). 409 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shozushima M..: "The characteristics of FDG and ^<11>C Methionine uptake by proliferating tumor cells in vivo"NMCC Annual report. vol.9, in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Moriguchi H.: "Cell cycle dependency of FDG and 67Ga citrate uptake in HeLa S3 cells"Dent.J.Iwate Med.Univ.. 27(3). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakamura R.: "Failure in presumption of residual disease by quantification of FDG uptake in esophageal squamous cell carcinoma immediately after radiotherapy"Radiation Medicine. 20(4). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Izumisawa M.: "18FDG-uptake in a rabbit VX2 tongue cancer with lymph node metastasis"NMCC Annual report. Vol.8. 235-239 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kamata J.: "Evaluation of the viability of myocardium by magneto cardiograms in contrast with sugar-stress 18FDG-PET"NMCC Annual report. vol.8. 33-37 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Terasaki K.: "Construction of automated production system for [11C] choline using a Sep-Pak cartridge"NMCC Annual report. vol.8. 265-272 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shozushima M..: "Cell cycle phase-dependent uptake of FDG and 11C-Choline in HeLa cells"NMCC Annual report. vol.8. 240-246 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shozushima M..: "18FDG uptake changes along with DNA synthesis of HeLa cells"NMCC Annual report. vol.7. 273-278 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakamura R.: "Failure in presumption of residual disease by quantification of FDG uptake in esophageal squamous cell carcinoma immediately after radiotherapy"Radiation Medicine. 20(4). 181-186 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 守口 斉: "HeLa S3細胞に対するFDGと67Ga citrate集積の細胞周期依存性"岩手医科大学歯学部. 27(3). 270-278 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 鎌田潤也: "心磁図による生存心筋の評価〜糖負荷18FDG-PET画像との対比〜"NMCC共同利用研究成果報文集. 8巻. 31-33 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 泉澤 充: "ウサギVX2誘発舌癌転移リンパ節における18FDG集積"NMCC共同利用研究成果報文集. 8巻. 235-239 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 寺崎一典: "オンカラム法を用いた[11C]コリン自動合成装置の作成"NMCC共同利用研究成果報文集. 8巻. 265-272 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 小豆島 正典: "培養細胞へのFDG集積とCholine集積に対する細胞周期依存性"NMCC共同利用研究成果報文集. 8巻. 240-246 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 小豆島 正典: "培養細胞へのFDG集積とCholine集積に対する細胞周期依存性"NMCC共同利用研究成果報文集. Vol.8(in press). (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 小豆島正典: "口腔癌に対するFDG-PETのfalse positive症例の検討"NMCC共同利用研究成果報文集. Vol.7. 40-45 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 小豆島正典: "HeLa細胞のDNA合成過程における18FDG取り込み変化"NMCC共同利用研究成果報文集. Vol.7. 273-278 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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