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Relation of COX-2 in carcinogenesis and invasion and metastasis of oral cancer

Research Project

Project/Area Number 12671843
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 病態科学系歯学(含放射線系歯学)
Research InstitutionHyogo College of Medicine

Principal Investigator

SAKURAI Kazunari  Hyogo College of Medicine, Medical Department, Assistant Professor, 医学部, 講師 (30129118)

Co-Investigator(Kenkyū-buntansha) URADE Masahiro  Hyogo College of Medicine, Medical Department, Professor, 医学部, 教授 (70104883)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2001: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 2000: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsOral mucosa / Precancerous lesion / Squamous cell carcinoma / Invasion and Metastasis / COX-2 / DNA-Topoisomerase IIα / HPV type 16 / 18 / Immunohistochemistry / Topisomerase II alpha / 上皮異形成症 / 発癌 / TopoIIα / 口腔粘膜前癌病変 / 口腔扁平上皮癌 / COX-2蛋白
Research Abstract

The role of cyclooxygenase (COX)-2 in carcinogenesis, invasiveness and metastasis is unclear. In this research, to determine whether COX-2 expression could serve as an indicator of progression of tumor, invasiveness and metastasis in oral carcinoma, we mainly examined the immunohistochemical expression of COX-2 protein with the analysis on cell proliferative activity in 221 cases of oral precancerous disease and oral cancer including carcinomas in situ (CISs), invasive squamous cell carcinomas and undifferentiated carcinomas.
As the results, normal squamous epitheliums that used as control showed no COX-2 expression. In contrast, approximately 60% of intraepithelial dysplasias, 83% of CISs, and all of invasive carcinomas demonstrated increase COX-2 expression with elevated DNA-Topoisomerase (Topo) IIα laballing index (LI) as cell proliferating marker. As an interestingly fact, a high intensity of COX-2 expression was observed in the developmental foci with tumor invasion of oral cancer. … More Increased expression of COX-2 protein correlated with elevated DNA-TopoIIαLI, indicating that COX-2 may contribute to malignant transformation and tumor growth. Furthermore, the patients with expression of both COX-2 and DNA-TopoIIα showed poor outcome. Therefore, it was considered that the COX-2 related to tumor invasiveness, and was suggested that the COX-2 expression become a possible indicator in the outcome in patients with oral cancer.
On the other hand, we examined the relationship between the expression of COX-2 protein with cell proliferative activity in regional lymph nodes metastasis and outcome inpatients with oral cancer. Expression of COX-2 protein and DNA-TopoIIα activity were evaluated by immunohistochemical technique in eacn 70 of oral squamous cell carcinomas and undifferentiated carcinomas of primary with or without regional lymph node (LN) metastatic lesions. Twenty normal oral squamous epitheliums were used as control. In the result, increased COX-2 expression withelevated DNA-TopoIIαLI. Increased COX-2 expression with elevated DNA-TopoIIα LI in the primary tumors was more frequent and intense in the LN(+) group than in the LN(-) group (p<0.05). In the LN(+) group, 56 (80%) patients also showed a similar increased COX-2 expression with elevated DNA-TopoIIαLI in the metastatic lesions, compared with the primary lesions (p<0.01). Furthermore, 42 (75%) patients with increased COX-2 expression with elevated DNA-TopoIIαLI had poor outcome. Therefore, it was suggested that the COX-2 expression become a possible indicator in the metastasis of oral cancer and might reflect on the outcome of the patients with oral cancer. Less

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] 櫻井一成, 黒田純子, 橋谷 進, 西村則彦, 野口一馬, 岸本裕充, 浦出雅裕: "口腔扁平上皮癌の原発、転移巣におけるCyclooxygenase(COX)-2蛋白発現の比較検討"日本口腔科学会雑誌. 51. 366-373 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sakurai K, Kuroda J, Hashitani S, Nishimura N, Noguchi K, Kishimoto H and Urade M: "Comparison of expression of cyclooxygenase (COX)-2 protein in primary and metastatic lesion of oral squamous cell carcinoma"J Jpn Stomatol Soc. 51(6). 366-372 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 櫻井一成, 黒田純子, 橋谷 進, 西村則彦, 野口一馬, 岸本裕充, 浦出雅裕: "口腔扁平上皮癌の原発、転移巣におけるCyclooxygenase(COX)-2蛋白発現の比較検討"日本口腔科学会雑誌. 51巻6号. 366-373 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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