| Project/Area Number |
12671851
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
MYOKAI Fumio Okayama University, Graduate School of Medicine and Dentistry, Professor, 大学院・医歯学総合研究科, 助手 (50263588)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIMURA Fusanori Okayama University, Dental Hospital, assistant professor, 歯学部・附属病院, 講師 (80208222)
TAKASHIBA Shogo Okayama University, Graduate School of Medicine and Dentistry, associate professor, 大学院・医歯学総合研究科, 助教授 (50226768)
MURAYAMA Yoji Okayama University, Graduate School of Medicine and Dentistry, Professor, 大学院・医歯学総合研究科, 教授 (50029972)
KOHNO Takayuki Okayama University, Dental Hospital, assistant, 歯学部・附属病院, 助手 (80284074)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2000: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | root resorption / PDL cell / mechanical stress / subtraction / 機械的剌激 |
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| Research Abstract |
Using subtractive hybridization, we isolated genes from human periodontal ligament (PDL) cells that were differentially expressed in response to mechanical stress. MRG X gene which related to morality factor 4 gene was cloned from a human cDNA library by subsequent screening. It belongs to a novel family of transcription factors regulating cellular proliferation and senescence. Cellular proliferation is initiated by growth factor binding to specific receptors to initiate signal transduction. Transcripts for the MRG X gene were increased following mechanical stress in cultured PDL cells. The target genes for the MRG X are unknown. Transforming growth factor (TGF)-β1 is mitogenic to PDL cells and regulates the osteoblast-like phenotype of PDL cells. TGF-β1 and its type I receptor (TβR-I) genes were co-expressed following mechanical stress in cultured PDL cells. To examine the effects of the MRG X on the expression of TGF-β1 gene, we performed functional studies aimed at interfering with MRG X mRNA production in cultured PDL cells. Inhibition of MRG X gene mRNA expression in PDL cells resulted in transcription of both the TGF-β1 and TβR-I genes. An application of mechanical stress resulted in a marked reduction of the transcription of TGF-β1 gene in MRG X antisense-expressing cells. The expression level of endogenous MRGX mRNA was elevated by the mechanical stress in the cells. These findings suggest that MRG X acts as a negative regulator of transcription for both TGF-β1 gene in PDL cells.
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