| Project/Area Number |
12671857
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
SAITO Takashi Health Sciences University of Hokkaido School of Dentistry, Department of Operative Dentistry and Endodontology, Assistant Professor, 歯学部, 講師 (40265070)
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| Co-Investigator(Kenkyū-buntansha) |
BESSHO Kazuhisa Kyoto University, Graduate School, Assistant Professor, 大学院・医学研究科, 講師 (90229138)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | Dentin induction / rhBMP-2 / E. Coll / Pulp Cells / Pule Capping / E.coli-derived rhBMP-2 variant / 歯髄 / 硬組織形成誘導 / E.coli-derived rhBMP-2 |
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| Research Abstract |
The final goal of this project is to develop new bioactive pulp capping agent using rhBMP 2. We examined mRNA expressions of pulp cells stimulated by E. Coli-derived rhBMP-2 variant using quantitative PCR.
We got pulp tissue from extracted fresh wisdom tooth, and obtained pulp cells by out-growth method. We cultured the pulp cells with rhBMP-2 of 0, 10, 100 and 1000 ng/ml without serum, and measured various mRNA expressions using LightCycler (Roche, Germany). As a result, rhBMP-2 enhanced dentin sialo phosphoprotein mRNA expression, that is thought to be marker of odontoblast differentiation in a concentra ion dependent manner. Therefore, we found that rhBMP-2 enhanced the differentiation of pulp cells into odontoblasts, and it is suggested that effective pulp-conservative agent would be produced by using rhBMP-2.
Next We implanted rhBMP-2/ type I collagen/ hydroxyapatite/ phosphophoryn composite into subcutaneous connective tissue or onto scarpture of rat. Then we sacrificed the rats, and analyzed the implants biochemically and historically As a result, we found that rhBMP-2/ type I collagen/ hydroxyapatite/ phosphophoryn composite induced bone formation strongly compared with control group.
It was indicated that rhBMP-2/ type I collagen/ hydroxyapatite/ phosphophoryn composite can be effective pulp capping agent.
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