• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development and Histopathologic Features of Odontoclastic Resorption on the Internal Dentin Wall after Replantation of Rat Molars

Research Project

Project/Area Number 12671866
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionThe Nippon Dental University

Principal Investigator

SHIMAZU Yoshihito  The Nippon Dental University, School of Dentistry at Tokyo, Department of Pathology, Assistant Professor, 歯学部, 助手 (10297947)

Co-Investigator(Kenkyū-buntansha) KITAMURA Kazuo  The Nippon Dental University, School of Dentistry at Tokyo, Department of Pathology, Lecturer, 歯学部, 講師 (00224965)
YAGISHITA Hisao  The Nippon Dental University, School of Dentistry at Tokyo, Department of Pathology, Lecturer, 歯学部, 講師 (50256989)
TAYA Yuji  The Nippon Dental University, School of Dentistry at Tokyo, Department of Pathology, Lecturer, 歯学部, 講師 (30197587)
AOBA Takaaki  The Nippon Dental University, School of Dentistry at Tokyo, Department of Pathology, Professor, 歯学部, 教授 (30028807)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywordsrat molars / osmotic pump / odontoclast / osteoclast / growth factor / bisphosphonate / cell adhesion / extracellular matrix / 外傷刺激 / 歯髄・歯根膜 / 歯質吸収 / 象牙芽細胞 / 細胞増殖・分化 / 細胞基質間接着
Research Abstract

The etiology, pathogenesis and prognosis of internal root resorption in human dentition remain to be better understood. We aimed at establishing rat molar model combining an osmotic pump for local-infusion of chemical agents into the periapical region of the mesial root of upper first molars. Male Sprague-Dawley rats (6-wk-old, 200g b.w.) were used. At the surgical operation under general anesthesia, the apical end of the medial root was removed with the aid of a small dental bar (apicoectomy), leaving a drilled hole (around 0.8mm in diameter) through cortical bone. The pump loading 200 μL of an agent-solubilized solution (or vehicle only as control) was implanted subcutaneously on the dosal area and the cannula was placed into the drilled bony hole in the molar region (local-infusion). The results obtained during the funding period prove that rat molar model is useful for investigation of internal dentin resorption. Histologic examination of the replanted molars showed the occurrence … More of resorption lacunae accompanying TRAP-positive multinuclear cells on the internal dentin wall around day 7 after tooth replantation. Resorption lacunae were classified into A and B types, which appeared to correspond to surface and inflammatory types of external root resorption, respectively. The essential event responsible for the development of internal dentin resorption is the replacement of the innate pulp tissue with the ingrowth of granulation tissue and the assembly of a new cell population comprising macrophages and fibroblastic stromal cells. The initial attachment of odontoclasts to the predentin wall does not necessarily need preconditioning with OPN and appears to be mediated by alternative recognition mechanisms. The overal results support the hypothesis that the innate pulp of rat molars possesses in potency inhibitory functions against undesirable resorption activities, but after impairment of the innate pulp environment after tooth replantation, a new paradigm of cell-matrices and cell-cell interactions may act cooperatively to induce clastic acitivities on the internal predintin/dentin wall. Less

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Sempuku H, et al.: "Effects of human interleukin-18 and interleukin-12 treatment on human lymphocyte engraftment in NOD-scid mouse"Immunology. 107. 232-242 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shimazu Y, et al.: "Immunodetection of osteopontin at sites of resorption in the pulp of rat molars"J Histochem Cytochem. 50. 911-921 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 佐藤俊一郎, 他: "FGF2、heparin、dexamethasone、bisphosphonateによるラット破骨細胞の分化・活性化の制御:体内埋入型浸透圧ポンプによるラット臼歯根断端部への生理活性因子の持続投与"日歯保存誌. 45. 253-272 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shimazu Y, et al.: "Occurrence and histopathologic features of odontoclastic resorption on the internal dentin wall after reimplantation of rat molars"Oral Med Pathol. 6. 59-64 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 島津徳人, 他: "ラット臼歯再植後の象牙芽細胞の運命:象牙芽細胞は虚血状態に耐えうるか?"日歯保存誌. 44. 350-360 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 佐藤俊一郎, 他: "ラット臼歯外傷モデルにおける歯髄・歯根膜組織の創傷治癒:体内埋入型浸透圧ポンプによる臼歯根尖部への薬剤持続投与"日歯保存誌. 44. 361-371 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sempuku H, et al.: "Effects of human interleukin-18 and interleukin-12 treatment on human lymphocyte engraftment in NOD-scid mouse."Immunology. 107. 232-242 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shimazu Y, et al.: "Immunodetection of osteopontin at sites of resorption in the pulp of rat molars."J Histochem Cytochem. 50. 911-921 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sato S, et al.: "Regulatory effects of FGF2, heparin, dexamethasone,and bisphosphonate on differentiation and activation of rat osteoclasts' Use of an osmotic pump for continuous infusion of bioactive agents into apicoectomized regions of rat molars."Jpn J Conserv Dent. 45. 253-272 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shimazu Y, et al.: "Occurrence and histopathologic features of odontoclastic rescjrption on the internal dentin wall after reimplantation of rat molars."Oral Med Pathol. 6. 59-64 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shimazu Y, et al.: "Fate of odontoblasts after replantation of rat molars: Could the cells survive under ischemic conditions?"Japan J Conserv Dent. 44. 350-360 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sato S, et al.: "Wound healing of the pulp/periodontal tissue of traumatized rat molars: Use of an osmotic pump for continuous infusion of chemical agents into the periapical region."Japan J Conserv Dent. 44. 361-371 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sempuku H, et al.: "Effects of human interleukin-18 and interleukin-12 treatment on human lymphocyte engraftment in NOD-scid mouse"Immunology. 107. 232-242 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shimazu Y, et al.: "Immunodetection of osteopontin at sites of resorption in the pulp of rat molars"J Histochem Cytochem. 50. 911-921 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 佐藤俊一郎, 他: "FGF2、heparin、dexamethasone、bisphosphonateによるラット破骨細胞の分化・活性化の制御:体内埋入型浸透圧ポンプによるラット臼歯根断端部への生理活性因子の持続投与"日歯保存誌. 45. 253-272 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shimazu Y, et al.: "Occurrence and histopathologic features of odontoclastic resorption on the internal dentin wall after replantation of rat molars"Oral Med Pathol.. 6. 59-64 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Taya Y, et al.: "Spatio-temporal expression patterns of gelatinolytic activity in mouse secondary palates"Oral Med Pathol.. 6・1. 37-41 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sato K, et al.: "Sexual dimorphism of porcine amelogenins : male-specific amelogenins have strong adsorption properties onto apatite crystals"Odontology. 89. 28-33 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 島津徳人, 他: "ラット臼歯再植後の象牙芽細胞の運命:象牙芽細胞は虚血状態に耐えうるか?"日歯保存誌. 44・2. 350-360 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 佐藤俊一郎, 他: "ラット臼歯外傷モデルにおける歯髄・歯根膜組織の創傷治癒:体内埋入型浸透圧ポンプによる臼歯根尖部への薬剤持続投与"日歯保存誌. 44・2. 361-371 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 島津徳人 他: "ラット臼歯再植後の象牙芽細胞の運命:象牙芽細胞は虚血状態に耐えうるか?"日歯保存学誌. 44(印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 大島克郎 他: "ラット臼歯への外傷刺激にともなう歯髄・歯根膜・歯肉組織での細胞増殖活性の経日的変動:免疫組織化学による検討."日歯保存学誌. 43. 842-851 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 佐藤俊一郎 他: "ラット第1臼歯近心根の根尖切除後にみられた歯髄・歯周組織の再生修復応答."日歯保存学誌. 43. 134-143 (2000)

    • Related Report
      2000 Annual Research Report

URL: 

Published: 2000-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi