| Project/Area Number |
12671886
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
補綴理工系歯学
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
HOSOKAWA Ryuji University Dental Hospital, Hiroshima University, Assistant Professor, 歯学部・附属病院, 講師 (60211546)
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| Co-Investigator(Kenkyū-buntansha) |
YAMANAKA Takenori University Dental Hospital, Hiroshima University, Research Associate, 歯学部・附属病院, 助手 (20325202)
TAJI Tsuyoshi University Dental Hospital, Hiroshima University, Research Associate, 歯学部・附属病院, 助手 (80284214)
AKAGAWA Yasumasa Faculty of Dentistry, Hiroshima University, Professor, 歯学部, 教授 (00127599)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | periodontal ligament-derived epithelial cells / rests of Malassez / periodontal ligament-derived fibroblast cells / serum-free culture / fibroblast growth factor / fibroblast growth factor receptor / 歯周靭帯由来線維芽細胞 / 歯周靭帯由来上皮細胞 / growth assay試験 |
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| Research Abstract |
Human periodontal ligament-derived epithelial cells (Epithelial rests of Malassez : ME) are derived from Hertwig's epithelial root sheath. In this study, we have developed a serum-free culture system of ME. The mitogenic effect of fibroblast growth factor (FGF) in the ME, human periodontal ligament-derived fibroblast cells (PLF) were investigated by serum-free growth assay. In addition, we demonstrated the expression of FGF receptor (FGFR) in the ME and the expression of FGF in the PLF by reverse transcription-polymerase chain reaction (RT-PCR), PCR-Southern hybridization, immunohistochemical examination, western blotting and ribonuclease protection assay (RPA). Both FGF-1 and FGF-7/keratinocyte growth factor (KGF) stimulated cell growth of the ME. On the other hand, both FGF-1 and FGF-2 stimulated cell growth of the PLF. RT-PCR and PCR-Southern analysis revealed that the ME expressed FGFR2-(IIIb) mRNA but not mRNAs for FGFR1-(IIIc), FGFR2-(IIIc), FGFR3-(IIIb), FGFR3-(IIIc) and FGFR4. An immunohistochemical analysis of FGF-7/KGF revealed that immunoreactive FGF-7/KGF were detected predominantly in the cytoplasm of which type of cell. PCR-Southern, western blotting and RPA revealed that the PLF expressed FGF-7/KGF mRNA. These results suggest that the FGF-FGFR2-(IIIb) system play an important role in the growth and differentiation of ME. In addition, endogenously produced FGFs work as paracrine interactive regulators of the growth and differentiation between ME and PLF.
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