|Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2000: ¥1,600,000 (Direct Cost: ¥1,600,000)
An experiment was done by using peridontal ligament(P.D.L.) of beagle dogs for the purpose of the long-term preservation of tooth transplant and replantation.
First, tooth were preserved with four kinds of preservation solutions(University of Wisconsin solution, Euro-collins solution, Dulbecocos Modified Eagles medium and Isotonic sodium chloride) in 4℃. In result, UW was the best was shown for one week after the preservation as a result of looking up P.D.L.cellular activation with out-growth method.
Next, the reference of the preservation condition was done. The activation of PDLcell was looked up with out-growth method after -2℃ which was Hyo-on preservation which was -80℃ as to preservation temperature of the cell which Was 4℃ as to internal organs preservation temperature was set up and a tooth was kept in each temperature in the UW. As for the preservation example as well, P.D.L cell which kept Hyo-on in that result UW recognized cellular multiplication for 21 days.
So, tooth replantation experiment of the kept tooth was done by using beagle dogs. A preservation priod was 4＆6 weeks Presavation temperature was set up in 4℃, -80℃, -2℃. After 8weeks, dogs were slaughtered and histological examination, was done. After 4weeks preserved in Hyo-on(-2℃)tooth were replanted and it worked like healthy tooth.
The research of the freezing preservation was done to keep P.D.L. cellular multiplication for more long term. 3 kinds of freezing prevention materiaI (trehloce glycerol DMSO) were added to the preservation solutions(UW), 0% &10%. Preservation temperature was -2℃&-80℃, after preservation the analysis of the cell multiplication examination, movement Noh play examination was done. A good record was shown in the order of U.W+DMSO in -80C. UW+ glycerol and U.W+trehaloce in -80 C.