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DEVELOPMENT OF DENTAL IMPLANTS FOR IMPROVING MICROBIAL ADHESION

Research Project

Project/Area Number 12671903
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 補綴理工系歯学
Research InstitutionTOKYO DENTAL COLLEGE

Principal Investigator

YOSHTNARI Masao  TOKYO DENTAL COLLEGE, DEPARTMENT OF DENTISTRY, ASSISTANT PROFESSOR, 歯学部, 助教授 (10085839)

Co-Investigator(Kenkyū-buntansha) INOUE Takashi  TOKYO DENTAL COLLEGE, DEPARTMENT OF DENTISTRY, PROFESSOR, 歯学部, 教授 (20125008)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
KeywordsDental implant / Biological seal / Oralbacteria / Adhesion / Antibacterial activity / Titanium / Surface modification / Dry process
Research Abstract

The peri-implant connective tissue fiber could be expected to make a biological seal which serves to prevent the penetration of periodontally pathologic chemical and bacterial substances. In order to those demands, we evaluated extension and spread of fibroblasts and epithelial cells, and oral bacterial adherence and antibacterial activity on surface-modified titanium. The extension and spread of fibroblasts and epithelial bells were critically influenced by the pore size of 1.2-3.0 u.m in diameter on millipore filters. Our observation of in vitro experiment also suggest that a range of 50 to 100 μm in pore size is most critical for the connective tissue cells to migrate and orientated right angle to the implant surface similar tosharpey's fibers may influence a biological seal around the implant. This is the reason why we create the pores around the neck of the implant surface.
The influence of surface modifications to titanium on the initial adherence of P. gingivalis ATCC33277 and A. … More actinomycetemcomitans ATCC437 1 8 was evaluated. Surface modifications were carried out with dry processes that including ion implantation (Ca^+, N^+, F^+), oxidation (anode oxidation, titania spraying), ion plating (TiN, alumina),and ion beam mixing (Ag, Sn, Zn, Pt) with Ar+ on polished pure titanium plates. Comparatively large amounts of.P.gingivalis and A. actinomycetemcomitans adhered to polished titanium plates. The degree of P. gingivalis adhesion showed a positive correlation with surface energy and the amount of calcium-ion adsorption. Both P.gingivalis and A. actinomycetemcomitans adherence increased on calcium-implanted surfaces compared to polished titanium surfaces, whereas P. gingivalis adherence was remarkably decreased on alumina-coated surfaces. These findings indicate that titanium implants exposed to the oral cavity requiresurface modification to inhibit the adherence of oral bacteria, and that surface modification with a dry process is useful in controlling the adhesion of oral bacteria as well as ensuring resistance against wear. The antibacterial effect of surface modifications to titanium on those bcteria was also evaluated. F+-implanted specimens significantly inhibited the growth of both P. gingivalis and A. actinomycetemcomitans. than the polished titanium. The other surface-modified specimens did not exhibit effective antibacterial activity against both bacteria. No release of the fluorine ion was detected from F-Jmplanted specimens under dissolution testing. This result and the characterization of the F^+-implanted surfaces suggested that the possible antibacterial mechanism of the F^+-implanted specimen was caused by the formation of a metal fluoride complex on the surfaces. In addition, F^+-implanted surfaces did not inhibit the proliferation of fibroblast L929-cells. These findings indicate that surface modification by means of a dry process is useful in providing antibacterial activity of oral bacteria to titanium implants exposed to the oral cavity. Less

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (52 results)

All Other

All Publications (52 results)

  • [Publications] Yoshinari, M., Matsuzaka, K., Inoue, T., Oda Y., Shimono, M.: "Effects of multi-grooves surfaces on fibroblast behavior"J Biomed Master Res. 65A(in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshinari, M., Matsuzaka, K., Inoue, T., Oda Y., Shimono, M.: "Bio-functionalization of titanium surfaces for dental implants"Materials Transactions. 43・10. 2494-2501 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshinari, M., Oda Y., Inoue, T., Matsuzaka, K., Shimono, M.: "Bone response to calcium phosphate-coated and bisphosphonate-immobilized titanium implants"Biomaterials. 23・14. 2879-2885 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hayakawa, T., Yoshinari, M., Nemoto, K., Wolke, J.G.C., Jansen, J.A.: "Trabecular bone response to surface roughened and calcium phosphate (Ca-P) coated titanium implants"Biomaterials. 23・4. 1025-1031 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshinari, M., Oda Y., Inoue, T., Shimono, M.: "Dry-process surface modification for titanium dental implants"Metallurgical and Materials Transactions A. 33A・3. 511-519 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Okumura A, Goto M, Goto T, Yoshinari M, Masuko S, Katsuiki T, Tanaka T: "Substrate affects the initial attachment and subsequent behavior of human osteoblastic cell (Saos-2)"Biomaterials. 22・16. 2263-2271 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshinari, M., Oda, Y., Kato, T., Okuda, K.: "Influence of surface modifications to titanium on antibacterial activity in vitro"Biomaterials. 22・14. 2043-2048 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshinari, M., Oda, Y., Ueki, H., Yokose, S.: "Immobilization of bisphosphonates on surface modified titanium"Biomaterials. 22・7. 709-715 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshinari, M., Oda, Y., Kato, T., Okuda, K., Hirayama, A.: "Influence of surface modifications to titanium on oral bacterial adhesion in vitro"Journal of Biomedical Materials Research. 52・2. 388-394 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshinari M, Oda Y: "Structural Biomaterials for the 21st Century, Titanium surface modification with dry process for dental implants(著分担)"The Minerals, Metals & Materials Society. 288 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 吉成正雄: "界面ハンドブック,第15章 生体材料 第3節 材料の表面改質およびその応用 リン酸カルシウムコーティング(著分担)"エヌ・ティー・エス. 1208 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshinari M, Oda Y, Inoue T, Shimono M.: "Dry-Process surfaceI modification for titanium dental implants"Metallurgical and Materials Transactions A. 33. 511-519 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshinari M, Matsuzaka K, Inoue T, Oda Y, Shimono M: "Bio-firactionalization of titanium surfaces for dental implants"Materials Transactions. 43(10). 2492-2501 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshinari M, Matsuzaka K, Inoue T, Oda Y, Shimono M.: "Effects ofmulti-erooved surfaces on fibroblast behavior"J Biomed Mater Res. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shiraiwa M, Goto T, Yosfainari M, Koyano K, Tanaka K.: "A study ofthe initial attachment and subsequent behavior of rat oral epithelial cells culturedon titanium"J Periodontol. 73. 852-860 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nagai M, Hayakawa T, Fukatsu A, Yamamoto M, Fukumoto M, Nagahama F, Mishima H, Yoshnari M, Nemoto K, Katoh T.: "In vitro studv on collagen coatine of titanium imolant"Dent Mater J. 21. 250-260 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshinari M, Oda Y, Inoue T, Matsuzaka K, Shimono M.: "Bone response to calcium phosphate-coated and bisphosphonate-immobilized titanium imolants"Biomaterials. 23. 2879-2885 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hayakawa T, Yoshinari M, Nemoto K, WoIke JGC, Jansen JA: "Trabecular bone response to surface roughened and calcium phosphate CCa-P) coated titanium imolants"Biomaterials. 23. 1025-1031 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ikeda H, Shiraiwa M, Yamaza T, Yoshinari M, Kido MA, Ayukawa Y, Inoue T, Koyano K, Tanaka T.: "Difference in the penetration of horseradish peroxidase tracer as a foreign substance into the peri-imblant epithelium or junctional etntflelium of rat gineivae"Clinical Oral Implants Res. 13. 243-251 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshinari M, Oda Y, Ueki H, Yokose S.: "Immobilization of bisphosphonates on surface modified titanium"Biomaterials. 22. 709-715 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshinari M, Oda Y, Kato T, Okuda K.: "Influence of surface modifications totitanium on antibacterial activity ui vitro"Biomaterials. 22. 2043-2048 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Okumura A, Goto M, Goto T, Yoshinari M, Masuko S, Katsuki T,Tanaka T.: "Substrate affects the initial attachment and subsequent behavior of human osteoblastic cell (Saos-2)"Biomaterials. 22. 2263-2271 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshinari M, Oda Y.: "Titanium surface modification with dry process for dental implants"Structural Biomaterials for the 21st Century 1st Ed, TMS publications, Warrendale,Pennsylvania. 129-136 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hayakawa T, Yoshinari M, Nemoto K, Wolke JGC, Jansen JA.: "Effect ofsurface roughness and calcium phosphate coating on implant bone response"Clinical Oral Impl Res. 11. 296-304 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ikeda H, Yamaza T, Yoshinari M, Ohsaki Y, Ayukawa Y, Kido MA,Inoue T, Shimono M, Koyano K, Tanaka T.: "Ultrastructural and immunoelectron microscopicstudies of the peri-implant epitheliumimplant (TJ-6A1-4V) interface of rat maxilla"J Periodontology. 71. 961-973 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshinari M, Oda Y, Kato T, Okuda K, Hirayama A.: "Influence of surface modifications to titanium on oral bacterial adhesion in vitro"J Biomed Mater Res. 52. 388-394 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshinari, M., Matsuzaka, K., Inoue, T., Oda Y., Shimono, M.: "Effects of multi-grooved surfaces on fibroblast behavior"J Biomed Mater Res. 65A(in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yoshinari, M., Matsuzaka, K., Inoue, T., Oda Y., Shimono, M.: "Bio-functionalization of titanium srfaces for dental implants"Materials Transactions. 43・10. 2494-2501 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yoshinari, M., Oda Y., Inoue, T., Matsuzaka, K., Shimono M.: "Bone response to calcium phosphate-coated and bisphosphonate-immobilized titanium implants"Biomaterials. 23・14. 2879-2885 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hayakawa, T., Yohinari, M., Nemoto, K., Wolke, J.G.C., Jansen, J.A: "Trabecular bone response to surface roughened and calcium phosphate (Ca-P) coated titanium implants"Biomaterials. 23・4. 1025-1031 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yoshinari, M., Oda Y., Inoue, T., Shimono, M.: "Dry-process surface modification for titanium dental implants"Metallurgical and Materials Transactions A. 33A・3. 511-519 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Okumura A, Goto M, Goto T, Yoshinari M, Masuko S, Katsuiki T, Tanaka T: "Substrate affects the initial attachment and subsequent behavior of human osteoblastic cell (Saos-2)"Biomaterials. 22・16. 2263-2271 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yoshinari, M., Oda, Y., Kato, T., Okuda, K.: "Influence of surface modifications to titanium on antibacterial activity in vitro"Biomaterials. 22・14. 2043-2048 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yohinari, M., Oda, Y., Ueki, H., Yokose, S.: "Immobilization of bisphosphonates on surface modified titanium"Biomaterials. 22・7. 709-715 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yoshinari, M., Oda, Y., Kato, T., Okuda, K., Hirayama, A.: "Influence of surface modifications to titanium on oral bacterial adhesion in vitro"Journal of Biomedical Materials Research. 52・2. 388-394 (2000)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yohinari M, Oda Y: "Structural Biomaterials for the 21st Century, Titanium surface modification with dry process for dental implants (著分担)"The Minerals, Metals & Materials Society. 288 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 吉成正雄: "界面ハンドブック、第15章 生体材料 第3節 材料の表面改質およびその応用 リン酸カルシウムコーティグ (著分担)"エヌ・ティー・エス. 1208 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hayakawa, T., Yoshinari, M., Nemoto, K., Wolke, J.G.C., Jansen, J.A.: "Trabecular bone response to surface roughened and calcium phosphate(Ca-P) coated titanium implants"Biomaterials. 23・4. 1025-1031 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Okumura A, Goto M, Goto T, Yoshinari M, Masuko S, Katsuiki T, Tanaka T: "Substrate affects the intial attachment and subsequent behabior of human osteoblastic cell(Saos-2)"Biomaterials. 22・16. 2263-2271 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yoshinari, M., Oda, Y., Kato, T., Okuda, K.: "Influence of surface modifications to titanium on antibacterial activity in vitro"Biomaterials. 22・14. 2043-2048 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yoshinari, M., Oda, Y., Ueki, H., Yokose, S.: "Immobilization of bisphosphonates on surface modified titanium"Biomaterials. 22・7. 709-715 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yoshinari, M., Oda, Y., Kato, T., Okuda, K., Hirayama, A.: "Influence of surface modifications to titanium on oral bacterial adhesion in vitro"Journal of Biomedical Materials Research. 52・2. 388-394 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yoshinari M, Oda Y: "Structural Biomaterials for the 21st Century, Titanium surface modification with dry process for dental implants(著分担)"The Minerals, Metals & Materials Society. 288 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 吉成正雄: "界面ハンドブック,第15章 生体材料 第3節 材料の表面改質およびその応用 リン酸カルシウムコーティング(著分担)"エヌ・ティー・エス. 1208 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yoshinari,M.,Oda,Y.,Ueki,H.,Yokose,S.: "Immobilization of bisphosphonates on surface modified titanium"Biomaterials. 22・7. 709-715 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yoshinari,M.,Oda,Y.,Kato,T.,Okuda,K.,Hirayama,A.: "Influence of surface modifications to titanium on oral bacterial adhesion in vitro"Journal of Biomedical Materials Research. 52・2. 388-394 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ikeda,H.,Yamaza,T.,Yoshinari,M., et al.: "Ultrastructural and immunoelectron microscopic studies of the peri-implant epithelium-implant (Ti-6Al-4V) interface of rat maxilla"Journal of Periodontology. 71・6. 961-973 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hayakawa,T.,Yoshinari,M., et al.: "Effect of surface roughness and calcium phosphate coating on implant bone response"Clinical Oral Implants Research. 11・4. 296-304 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 奥森直人,吉成正雄,小田豊: "予後不良により撤去されたハイドロキシアパタイトコーティング・インプラントの表面分析"歯科学報. 100・8. 737-753 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 宮山直也,吉成正雄,小田豊: "ドライプロセスによるインプラント用チタンの表面改質-表面の特性評価-"歯科材料・器械. 18・2. 109-121 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 吉成正雄,井上孝: "人工歯根の表面改質,"表面科学. 20・9. 613-620 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 井上孝,吉成正雄,下野正基: "歯科インプラント,第2章,1.インプラント/骨界面(著分担)"先端医療研究所. 447 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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