| Project/Area Number |
12671917
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
補綴理工系歯学
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| Research Institution | Fukuoka College of Health Sciences |
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Principal Investigator |
INOUE Yusuke Fukuoka College of Health Sciences, Dental Hygine, Assistant, 歯科衛生学科, 助教授 (20105688)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAI Tatsuhi Aichi Gakuin University, Dentistry, lecturer, 歯学部, 講師 (60167351)
OKAHATA Yoshio Tokyo Institute of Technoloty, Biomolecular Engineering, Professor, 生命理工学部, 教授 (80038017)
FUKUSHIMA Tadao Fukuoka Dental College, Dental Engineering, Assintant Professor, 歯学部, 助教授 (80084250)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2000: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | cytokine / BMP / lipid / polysaccharide / polymer film / サイトカン |
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| Research Abstract |
Sixteen kinds of artificial amphiphilic lipids were prepared from the reaction between n-alkyl alcohols (n-hexyl alcohol, n-octyl alcohol, n-decyl alcohol, n-dodecyl alcohol, n-tetradecyl alcohol, n-hexadecyl alcohol, n-octadecyl alcohol), amino acids (glycine, L-alanine, L-glutamic acid) and p-toluenesulfonic acid. Alginic acid or Heparin in 100 ml of distilled water or 50 wt% ethanolwater solution was sonicated for 5 min. Afterward, one of the prepared artificial amphiphilic lipids in 100 ml of distilled water or 50 wt% ethanol-water solution was added to 100 ml of polysaccharide suspension. The reaction mixture was stirred at 20℃ for three hours, polysaccharide-lipid complexes were collected by centrifugation, and were washed with distilled water or 50 wt% ethanol-water solutions. Finally, the white polysaccharide-lipid complex was freeze-dried. Polysaccharide-lipid complex was dissolved in chloroform /ethanol solution. The solution was evenly dropped on Teflon plate. After solvent was completely vaporated, polysaccharide-lipid complex film was obtained by removing from Teflon plate.Bone morphogenetic protein (BMP) was extracted from bovine demineralized bone matrix with 4 M guanidine hydrochloride.After concentration, an extract solution was dialyzed for several times. Finally aqueous BMP was obtained by gel-filtration. The lipid coated BMP was synthesized from the reaction between aqueous BMP solution and the artificial lipid (Didocyl-N-GluconoL-Glutamate). The polysaccharide-lipid complex films were almost dissolved 3 days after subcutaneous implantation in the backs of rats and showed no inflammatory reactions or inhibition of new tissue formation. Lipid coated BMP or the mixture of the polysaccharide-lipid complex film implanted in the femora of rat showed new bone formation, and no inflammatory reactions or inhibition of new tissue formation.
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