• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Microarray gene expression analysis for development prognostic factors for oral squamous cell carcinoma

Research Project

Project/Area Number 12671929
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionNiigata University

Principal Investigator

NAGATA Masaki  Graduate School of Medical and Dental Sciences, Niigata University, Assistant, 大学院・医歯学総合研究科, 助手 (10242439)

Co-Investigator(Kenkyū-buntansha) HOSHINA Hideyuki  Dental Hospital, Niigata University, Lecturer, 歯学部・附属病院, 講師 (30173587)
SAKU Takashi  Graduate School of Medical and Dental Sciences, Niigata University, Professor, 大学院・医歯学総合研究科, 教授 (40145264)
TAKAGI Ritsuo  Graduate School of Medical and Dental Sciences, Niigata University, Professor, 大学院・医歯学総合研究科, 教授 (20143795)
FUJITA Hajime  Graduate School of Medical and Dental Sciences, Niigata University, Assistant, 大学院・医歯学総合研究科, 助手 (60271805)
IDA Hiroko  Graduate School of Medical and Dental Sciences, Niigata University, Assistant, 大学院・医歯学総合研究科, 助手 (60293213)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥2,700,000 (Direct Cost: ¥2,700,000)
KeywordsOral sauamous cell carcinoma / Microarray / Gene expression profiling / Prognosis in metastasis / Diagnosis / lmmunohistochemistry / MMP / uPA / 口腔癌 / 診断法
Research Abstract

We performed gene expression profiling with 500 of the cancer related genes to develop diagnostic system of oral squamous cell carcinoma (OSCC). To eliminate biases caused by difference between tumors or measurements, we selected increased arid decreased genes in all OSCC cases. Unchangeable decreases were observed in gene expression ratio of Retinoic acid receptor gamma, Keratin family genes, and some molecules of desmosomes components against normal oral mucosa. Regular increases were observed in extracellular matrix (ECM)-degrading enzymes such as MMPs, uPA; ECMs such as Tenascin C and Fibronectin 1; Chemokines or transcription factors, such as MIG, IP-10, STAT1, BIGH3, that were induced by growth factors. Results of a cluster analysis indicated the similarity in gene expression patterns between those genes. The expression profile of cancer related genes in OSCC tissues seemed to reflect loss of epithelial characteristics, activated tissue destruction and formation of the cancer str … More oma, and disturbed phases in signal transudation systems. Comparison between groups with or without the involvement of lymphnode metastasis revealed significantly increased expression of MMP-1, uPA, CD44, Integrin alpha 3 and Paxillin as well as decreased mRNA levels of CD9 and IGFBP2. To confirm the results of gene expression analysis, we preformed immunohistochemistry for the genes that were suggested to associate with metastatic behavior of OSCCs. MMP-1, MMP-3 and uPA were co-localized extensively in inflammatory cells, endotherial cells and ECM structure, but no localization was indicated in tumor cells. Prominent staining of those gene products were observed on eosinophilic, round shaped mononuclear cells that were associated with degrading of surrounding collagen fibers and capillary structures. These histological findings corroborate the co-regulated expression and cooperative function of those ECM-degrading enzymes that were expected in gene expression analyzes. Striking correlation was demonstrated between MMP-1 mRNA revel and lymph node metastasis (U=0, p=0.001). MMP-1 could be an independent and accurate prognostic factor of lymph node metastasis for OSCC. By identifying commonly regulated and characteristic clusters of coexpressed genes, and by compeering the gene expression data with the extensive clinical data, and then by using immunohistochemistry against a subset of significant genes, surgical specimens with diverse backgrounds can be made accessible to extract valuable genes without prior dissection into components of tumor tissue. Less

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] 星名秀行: "進行頭頚部癌に対する温熱化学放射線療法の治療成績"癌と化学療法. 28(3). 331-336 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 星名秀行: "進行・再発頭頚部癌の温熱化学放射線療法-背景因子からみた治療成績-"頭頚部腫瘍181-186. 27(1). 181-186 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 永田昌毅: "DNAマイクロアレイを用いた口腔腫瘍の診断"新潟歯学会誌. 31(1). 35-37 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katsuhiro Nagashima: "Effect of local hyperthermia on metastases in oral squamous cell carcinoma"Int j Oral and Maxillofac Surg. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katsuhiro Nagaehima: "Effect of local hyperthermia on metastases in oral squamous cell carcinoma Oral Oncology 7 p460-463"Macmillan India Ltd. India. 610 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hoshina H: "Thermochemoradiotherapy for advanced or recurrent head and neck cancer : Analysis of clinical results and background variables. Advances in Hyperthermic Oncology 2001, pl27-131 Tanaka R. Ed."Koko-Do Niigata. 153 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hoshina, H.: "Clinical result of thermo-chemo-radiotherapy for advanced head and neck cancer"Jpn J Cancer Chemother. 28 (3). 331-336 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hoshina, H.: "Thermochemoradiotherapy for advanced or recurrent head and neck cancer: Analysis of clinical results and background variables"Head and Neck Cancer. 27 (1). 181-186 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagata, M. and Fujita, H.: "Diagnosis of the oral tumor using DNA microarray"Niigata Dent J. 31 (1). 35-37 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katsuhiro Nagashima: "Effect of local hyperthermia on metastases in oral squamous cell carcinoma"Int J Oral and Maxillofac Surg. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katsuhiro Nagashima: "Effect of local hyperthermia on metastases in oral squamous cell carcinoma Macmillan India Ltd. India"Oral Oncology. 7. 610 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hoshina, H.: "Thermochemoradiotherapy for advanced or recurrent head and neck cancer: Analysis of clinical results and background variables. Koko-Do Niigata"Advances in Hyperthermic Oncology, Tanaka R. Ed.. 153. (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 星名秀行: "進行頭頚部癌に対する温熱化学放射線療法の治療成績"癌と化学療法. 28(3). 331-336 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 星名秀行: "進行・再発頭頚部癌の温熱化学放射線療法 -背景因子からみた治療成績-"頭頚部腫瘍 181-186. 27(1). 181-186 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 永田 昌殻: "DNAマイクロアレイを用いた口腔腫瘍の診断"新潟歯学会誌. 31(1). 35-37 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Katsuhiro Nagashima: "Effect of local hyperthermia on metastases in oral squamous cell carcinoma"Intj Oral and Maxillofac Surg. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Katsuhiro Nagashima: "Effect of local hyperthermia on metastases in oral squamous cell carcinoma Oral Oncology 7 p460-463"Macmillan India Ltd. India. 610 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hoshina H: "Thermochemoradiotherapy for advanced or recurrent head and neck cancer: Analysis of clinical results and background variables. Advances in Hyperthermic Oncology 2001, p127-131 Tanaka R. Ed"Koko-Do Niigata. 153 (2001)

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2000-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi