| Project/Area Number |
12671935
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Shimane Medical University |
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Principal Investigator |
YOSHIMURA Yasuro School of Medicine, Shimane Medical University, Professor, 医学部, 教授 (50093480)
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| Co-Investigator(Kenkyū-buntansha) |
NAITO Shinichi School of Medicine, Shimane Medical University, Senior Resident, 医学部, 医員
OBARA Seiji School of Medicine, Shimane Medical University, Lecturer, 医学部, 講師 (70204279)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | Temporomandibular joint / Exo-utero development / Restriction of jaw movement / Growth factor / 成長因子 / マウス顎関節組織形成 / 開口制限 / 子宮外あるいは子宮内発育 |
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| Research Abstract |
By the maxillo-mandibular fixation, no significant systemic growth retardation of the embryo was not detected. But at the temporomandibular portion of the mouth movement restriction, the border between the cartilage of the condylar head and the calcified bone in the neck became unclear and diffuse macroscopically.
Deformed and disarranged cells were found in fee perichondrium of the condylar head, furthermore abnormal ossification was also observed between the proliferating and prehypertrophic zones and therefore discontinuity cell arrangement was characteristic. This finding suggested that jaw movement played an important role in the prenatal development of the temporomandibular joint. From the immunohistochemical staining and western blotting method of basic fibroblast growth factor (b-FGF) and its receptor (FGFR-3) revealed high stainability not in the superficial condylar chondrocytes but rather deeper proliferating and prehypertrophic zones at the embryonic day (E) 15.5, and E17.5. While in adults of both sex, b-FGF and FGFR-3 showed a high stainability in the superficial chondrocyte zone and gradually decreased towards to deeper zones. These molecules seemed to function in the mechanisms of calcification. Western blotting analysis of FGFR-3 in the normal growth showed a high stainability at the E 15.5, E 17.5 and at 11th day birth but in adult it revealed a weak stainability. B-FGF in the control group revealed a similar tendency but in adult at most little staining was observed.
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