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Study on angiogenesis in human oral aqueous cell carcinoma

Research Project

Project/Area Number 12671956
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionDepartment of Oral and Maxillofacial Surgery

Principal Investigator

SUGIMURA Masahitoj  Nara Medical University, Department of Oral and Maxillofacial Surgery, Professor, 医学部, 教授 (20028749)

Co-Investigator(Kenkyū-buntansha) KIRITA Tadaaki  Nara Medical University, Department of Oral and Maxillofacial Surgery, Associate Professor, 医学部, 助教授 (70201465)
KONISHI Noboru  Nara Medical University, Second Department of Pathology, Professor, 医学部, 教授 (20145832)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsOral / Squamous cell carcinoma / Angiogenesis / Id / Flt-4
Research Abstract

The helix-loop-helix proteins, Id-1, Id-2, and Id-3, have been demonstrated to inhibit the activity of transcription factors and to play an important role in regulating cell growth and tissue-specific differentiation. To elucidate the involvement of Id in human oral squamous cell carcinoma (OSCC), we examined 83 OSCCs for the expression of Id proteins by immunohistochemistry, 24 OSCCs for the expression of Id-1 mRNA by in situ hybridization (ISH), and 6 of the tumors and on cell lysates of 5 OSCC cell lines by Western blot analysis. A higher and more frequent expression of Id-1 and Id-2 proteins (67.5 % and 55.4 %, respectively) was found in human OSCC compared to expression of Id-3 (47.0 %), that Id protein expression, particularly Id-1 expression, correlated with histological grade. Western blot analysis detected Id-1 protein in 4 of 6 tumor samples and in all cell lines. ISH demonstrated strong cytoplasmic localization of Id-1 mRNA in tumor samples at significantly higher levels. Increased Id expression might be closely related to the control of cell proliferation and differentiation in human OSCC. On the other hand, the expression of VEGF, E-cadherin, or Ki-67 showed no significant correlation, but the factors such as tumor morphology, a tumor thickness greater than 4mm, and Flt-4 expression were significantly associated with development of neck metastasis in patients with OSCC.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] 桐田忠昭, 杉村正仁, 他: "進行舌癌症例に対する術前療法の原発巣・リンパ節に対する臨床病理学的効果と予後"癌の臨床. 46. 78-82 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nishimine, M., Konishi, N., Kirita, T., Sugimura, M., et al.: "Epigenetic alterations in methylation in oral squamous cell carcinoma cell lines detected by two-dimensional gel electropboresis"Int.J.Oncology. 17. 743-747 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kirita, T., Sugimura, M., et al.: "Consideration of extent of resection tor advance tongue carcinoma in view of responses to preoperative therapy"Oral Oncology. 8. 136-138 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kirita, T, Sugimura, M., et al.: "Prognostic value of response to preoperative chemoradiotheray and residual tumor grades in tongue carcinoma"Oral Surg.Oral Med.Oral Pathol.Oral Radiol.Endod.. 91. 293-300 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shiotani, H., Sugimura, M., et al.: "Increasedexpression of cyclooxygenase-2 protein in 4-nitroquinoline-1-oxide-induced rat tongue carcinomas and chemopreventive efficacy of a specific inhibitor, nimesuide"Cancer Res.. 61. 1451-1456 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamanaka, Y., Kirita, T., Sugiyama, M., et al.: "Epinephrine inhibits invasion of oral squamous carcmoma cells by modulating intracellular cAMP"Cancer Letters. 176. 143-148 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kirita T., Shimooka H., Nishimine M., Morisaki A, Mishima K, Morimoio, Y., and Sugimvira M.,: "Preoperative concurrent chemo radiotherapy in locally advanced tongue carcinoma-clinical and histopathologic effects of the primary tumor and lymph nodes, and the prognosis"Jpn. Cancer Clin.. 46. 78-82 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nishimine M., Konishi N., Yamamoto K., Nagai H., Emi M., Kirita T., Sugimura M.: "Epigenetic alterations in methylation in oral squamous cell carcinoma cell lines detected by two-dimensional gel electrophoresis"Int. J. Oncology. 17. 743-747 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kirita T., Ohgi K., Yamamoto K., Nishimine M., Sugimura M.: "Consideration of extent of resection for advance tongue carcinoma in view of responses to preoperative therapy"Oral Oncology. 8. 136-138 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kirita T., Shimooka H., Yamanaka Y., Tatebayashi S., Yamamoto K. Nishimine, M. Sugimura: "Prognostic value of response to preoperative chemoradotheray and residual tumor grades in tongue carcinoma"Oral Surg. Oral Med Oral Pathol. Oral Radiol. Endod. 91. 293-300 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shiotani H., Denda A., Yamamoto K, Kitayama W., Endoh T., Sasaki Y., Tsutusmi M., Sugimura M., and Konishi Y.,: "ncreased expression of cyclooxygenase-2 protein in 4-nitroquinoline-l-oxide-induced rat tongue carcinomas and chemo preventive efficacy of a specific inhibitor, nimesulide"Cancer Res.. 61. 1451-1456 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamanaka Y., Mammoto T., Kirita T., Mukai M., Mashimo T., Sugimura M., Kishi Y., Nakamura H.,: "Epinephrine inhibits invasion of oral squamous carcinoma cells by modulating intracellular cAMP"Cancer Letters. 176. 143-148 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 桐田忠昭, 杉村正仁, 他: "進行舌癌症例に対する術前療法の原発巣・リンパ節に対する臨床病理学的効呆と予後"癌の臨床. 46. 78-82 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nishimine, M., Konishi, N., Kirita, T., Sugimura, M., et al.: "Epigenetic alterations in methylation in oral squamous cell carcinoma cell line detected by two-dimensional gel electrophoresis"Int. J. Oncology. 17. 743-747 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kirita, T., Sugimura, M., et al.: "Consideration of extent of resection for advance tongue carcinoma in view of responses to preoperative therapy"Oral Oncology. 8. 136-138 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kirita, T., Sugimura, M., et al.: "Prognostic value of response to preoperative chemoradiotheray and residual tumor grades in tongue carcinoma"Oral Surg. Oral Med. Oral Pathol Radiol. Endod. 91. 293-300 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shiotani, H., Sugimura, M., et al.: "Increased expression of cyclooxygenase-2 protein in 4-nitroquinoline-1-oxide-induced rat tongue carcinomas and chemopreventive efficacy of a specific inhibitor, nimesulide"Cancer Res.. 61. 1451-1456 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamanaka, Y., Kirita, T., Sugimura, M.et al.: "Epineplirine inhibits invasion of oral squamous carcinoma cells by modulating intracellular cAMP."Cancer Letters. 176. 143-148 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nishimine,M.,Konishi,N.,Sugimura,M.,et al.: "Epigenetic alterations in methylation in oral squamous cell carcinoma cell lines detected by two-dimensional gel electrophoresis."Int.J.Oncology. 17. 743-747 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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