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Analysis of mechanism of bone formation stimulated by BMP in the tissue engineering

Research Project

Project/Area Number 12671969
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionNihon-University

Principal Investigator

SEKIWA Tadanobu  Nihon-University, School of Dentistry, Research Assistant, 歯学部, 助手 (00154659)

Co-Investigator(Kenkyū-buntansha) OIDA Shinichiro  Tsurumi University, School of Dentistry, Associate Prof., 歯学部, 助教授 (10114745)
INAGE Toshihiko  Nihon-University, School of Dentistry, Associate Prof., 歯学部, 助教授 (90096769)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordstissue engineering / bone formation / BMP / BMP receptor / HAP / CBFA-1 / TGF-β / receptor
Research Abstract

Recently, therapeutics using growth factors and extracellular matrix such as EMDOGAIN or BMP has been introduced dental practice. The growth factors therapeutics need a large amount of proteins and the mechanisms of healing are still obscure. In the present study, bone marrow stroma cells and HAP were used for bone formation of tissue engineering.
Materials and Methods
Wister rats weighting 100 g were used for this study. The stroma cells of bone marrow were isolated from bone marrow by aspiration of syringe. Stroma cells were cultured in alpha-MEM medium with HAP or CPC (calcium phosphate cement) by adding BMP. Stroma cells were cultured for 1 week. Then, cultured cells-HAP complex were transplanted in the artificial hole of calvaria.
Results
The cell-CPC complex showed stronger bone formation than that of cell-HAP complex. After 2 week of implantation of cell-CPC, thick layer of connective tissue was covered on implants. After 4 week the layer was calcified. Hence, in the implantation of the connective tissue layer was formed at 4 weeks after the implantation. TGF-β superfamily was expressed in the preosteoblasts, fibroblasts and osteoclasts around implants. These data suggests that TGF-β superfamily plays central role in ectopic bone formation.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] 寺門正明, 稲毛 稔彦他: "マウス顎顔面の成長におけるnogginの遺伝子発現"歯科基礎医学会雑誌. 42. 563-572 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 稲毛 稔彦: "歯科におけるBMPと骨誘導の発展性"日本歯科医師会雑誌. 53. 13-20 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Toshihiko Inage, Tadanobu Sekiwa: "Expression of Noggin Gene during Development of Maxillofacial Region in Mice"Japanese Journal of oral Biology. Vol.42, No.6. 563-572 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Toshihiko Inage: "Advances of BMP and bone indduction in"Journal Japanese dental association. Vol.53, No.11. 13-20

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 稲毛稔彦,関和忠信 他: "マウス顎顔面の成長におけるnogginの遺伝子発現"歯科基礎医学会雑誌. 42巻・6号. 563-572 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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