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Regulatory mechanism of the expression of TERT mRNA and telomerase activity in oral cancer

Research Project

Project/Area Number 12671981
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionFukuoka Dental College

Principal Investigator

OZEKI Satoru  Fukuoka Dental College, Oral and Maxillofacial Surgery, Professor, 歯学部, 教授 (80117077)

Co-Investigator(Kenkyū-buntansha) MIURA Masayoshi  Fukuoka Dental College, Oral and Maxillofacial Surgery, Assistant, 歯学部, 助手 (40320337)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordstongue cancer / telomerase / carcinogenesis / 4-NQO / human telomerase RNA (hTR) / hTEP1 / hTERT / human telomerase catalytic subunit
Research Abstract

Telomeres are considered one factor governing the life span of cells. In most immortalized cells or cancer calls, telomerase activity, which is one of the mechanisms responsible for elongation of shortened telomeres, is increased. Telomerase activity is therefore considered to play a crucial role in carcinogenesis. To study the role of telomerase activity in carcinogenesis in vivo, we investigated the relation between telomerase activity and histopathological findings of the tongue after treatment of DA rats with the carcinogen 4-NQO. Normal epithelium or epithelial hyperplasia of the tongue was found to have weak but detectable telomerase activity. Telomerase activity was considerably upregulated very early after the onset of malignant changes. Our results showed that telomerase was upregulated during early carcinogenesis of the tongue. This upregulation of telomerase activity may contribute to in vivo carcinogenesis. Telomerase activation is thought to be crucial for the sustained growth or progression of most cancer cells and its activation is an important step in carcinogenesis. Recently, three major subunits of human telomerase, hTR, hTEP1 and hTERT have been identified. In current study, we quantified the expression of these telomerase subunits mRNA in oral tissues with a RT-PCR method and evaluated the correlation between their expression and telomerase activity. Telomerase activity was detected in oral leukoplakia (75%), oral squamous cell carcinoma (S.C.C.) (66.7%). Both hTEP1 and hTR expression was detected in all tissue samples tested including normal gingiva. On the other hand, hTERT expression was detected in 12 of 22(54.5%) oral leukoplakia and SCC tissues and negative in normal gingiva samples. In addition, its expression of hTERT was correlated well with telomerase activity. hTERT expression and telomerase activation thus may be a early event in the process of oral carcinogenesis.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] 中野 伸一: "ラット4-NQO誘発舌癌の発癌過程におけるテロメラーゼ活性の制御機構に関する研究"日本口腔外科学会雑誌. 763-777 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shin-ichi Nakano: "Regulatory mechanism of telomerase activity in 4-NQO-induced carcinogenesis of rat tongue"Jpn.J Oral Maxillofac.Surg.. Vol.47. 763-769 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 中野 伸一: "ラット4-NQO誘発舌癌の発癌過程におけるテロメラーゼ活性の制御機構に関する研究"日本口腔外科学会雑誌. 47(12). 763-777 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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