| Project/Area Number |
12671983
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
矯正・小児・社会系歯学
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
MITIME Masato Hokkaido Univ., Grad. School of Dent. Med., Inst., 大学院・歯学研究科, 助手 (50261318)
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| Co-Investigator(Kenkyū-buntansha) |
KIKUIRI Takashi Hokkaido Univ., Grad. School of Dent. Med., Inst., 大学院・歯学研究科, 助手 (10322819)
SHIRAKAWA Tetsuo Hokkaido Univ., Dent. Hospital, Lec., 歯学部・附属病院, 講師 (00187527)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2001: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | trigeminal nucleus / neural stem cell / EGF / GFP / oligodendrocyte / MBP / bFGF / neuron / bFGF |
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| Research Abstract |
Donor cells for transplantation were derived from E15 striatum/subventricular zone tissue that was dissociated and cultured in a serum-free mediums supplemented with EGF (epidermal growth factor) or bFGF (basic fibroblast growth factor) from green fluorescent protein (GFP) transgenic mice. Neonatal host mice ranging in age from post natal day P1 to P3 were injected with 120000cells/2μl in either of the lateral cerebral ventricle or cisterna magna. Mice were perfused with 4% paraformaldehyde 2 to 8 weeks after transplantation. Brain sections were examined using light microscope with UV equipment and confocal microscopy.
Engrafted EGF-responsive neural precursor cells were mainly found within the white matter following the lateral cerebral ventricles transplantation. Confocal microscopy demonstrated engrafted GFP cells that were immunopositive for glial cells marker GFAP or CNPase. In the brainstem, EGF-responsive neural precursor cells engrafted robustly within the trigeminal spinal nucl
… More
eus and trigeminal spinal tract following cisternal transplantation. When EGF-responsive precursor cells were used as donor cells for transplantation into myelin-deficient shiverer mice, donor-derived cells were located within the caudal trigeminal areas and stained by myelin basic protein antibody. Transplantation of bFGF-responsive precursor cells into neonatal cisterna magna also resulted in their differentiation into glial cells in the spinal trigeminal tract. However some transplanted cells within the spinal trigeminal nucleus were immunoreactive for neuronal marker NeuN.
Our data indicate that EGF-responsive neural precursor cells can engraft robustly in the caudal trigeminal nucleus in the brainstem following cisternal transplantation in neonatal mice. The cells adopted glial phenotypes, and some functioned as oligodendrocytes. On the other hand, bFGF-responsive precursor cells differentiated into neuron in the spinal trigeminal nucleus. Such a transplanted strategy can rescue the abnormal phenotype of hosts at morphological, physiological and behavioral levels. Less
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